First record of Haemaphysalis concinna (Acari: Ixodidae) tick in the Khalkh Numrug basin of Eastern Mongolia

Haemaphysalis (Acari: Ixodidae) ticks remain poorly studied in Mongolia. Here, we report the first confirmed record of Haemaphysalis concinna Koch, 1844 in the Numrug Strictly Protected Area, Dornod Province, eastern Mongolia, based on both morphological characteristics and molecular analyses. This...

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Main Authors: Banzragch Battur, Batsaikhan Enkhtaivan, Davaajav Otgonsuren, Batbold Davkharbayar, Dalantai Munkhgerel, Tovuu Amgalanbaatar, Sandagdorj Narantsatsral, Batdorj Davaasuren, Myagmar Zoljargal, Punsantsogvoo Myagmarsuren, Orkhon Banzragchgarav, Noboru Inoue, Naoaki Yokoyama, Jinlin Zhou, Miroslav Bobek, Badgar Battsetseg
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Ticks and Tick-Borne Diseases
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Online Access:http://www.sciencedirect.com/science/article/pii/S1877959X25000858
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author Banzragch Battur
Batsaikhan Enkhtaivan
Davaajav Otgonsuren
Batbold Davkharbayar
Dalantai Munkhgerel
Tovuu Amgalanbaatar
Sandagdorj Narantsatsral
Batdorj Davaasuren
Myagmar Zoljargal
Punsantsogvoo Myagmarsuren
Orkhon Banzragchgarav
Noboru Inoue
Naoaki Yokoyama
Jinlin Zhou
Miroslav Bobek
Badgar Battsetseg
author_facet Banzragch Battur
Batsaikhan Enkhtaivan
Davaajav Otgonsuren
Batbold Davkharbayar
Dalantai Munkhgerel
Tovuu Amgalanbaatar
Sandagdorj Narantsatsral
Batdorj Davaasuren
Myagmar Zoljargal
Punsantsogvoo Myagmarsuren
Orkhon Banzragchgarav
Noboru Inoue
Naoaki Yokoyama
Jinlin Zhou
Miroslav Bobek
Badgar Battsetseg
author_sort Banzragch Battur
collection DOAJ
description Haemaphysalis (Acari: Ixodidae) ticks remain poorly studied in Mongolia. Here, we report the first confirmed record of Haemaphysalis concinna Koch, 1844 in the Numrug Strictly Protected Area, Dornod Province, eastern Mongolia, based on both morphological characteristics and molecular analyses. This region lies along the Mongolia-China border within the forest-steppe and steppe zones of the western Greater Khingan Mountains. Tick sampling was conducted at six sites (lakeshores, riverbanks, and marshy forests) between early April and mid-August over a three-year period.A total of 1014 H concinna specimens were collected, of which 148 (including two nymphs) were selected for DNA extraction. PCR amplifications targeting the mitochondrial 16S rRNA (∼455 bp) and Cox1 (∼820 bp) genes were performed. Eight samples were sequenced, with three 16S and two Cox1 sequences meeting quality thresholds. The sequences of both genes exhibited 99.8 % and 100 % identity, respectively, to H. concinna sequences from Russia (Cox1: PP851089.1, PP851095.1; 16S rRNA: KP866207.1) and China (Cox1: KR108863.1; 16S rRNA: ON097130.1) available in GenBank. This high level of sequence identity strongly supports the identification of the Mongolian samples as H. concinna. Species identification was further supported by morphological characteristics consistent with published descriptions of adult ticks. This record from the Khalkh Numrug basin represents the first verified occurrence of H. concinna in eastern Mongolia. These findings underscore the need for further research on the species’ ecology, host range, and potential public health significance in the region.
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series Ticks and Tick-Borne Diseases
spelling doaj-art-c38bcccd78be485f82ca3911628616712025-08-20T03:51:09ZengElsevierTicks and Tick-Borne Diseases1877-96032025-09-0116510252110.1016/j.ttbdis.2025.102521First record of Haemaphysalis concinna (Acari: Ixodidae) tick in the Khalkh Numrug basin of Eastern MongoliaBanzragch Battur0Batsaikhan Enkhtaivan1Davaajav Otgonsuren2Batbold Davkharbayar3Dalantai Munkhgerel4Tovuu Amgalanbaatar5Sandagdorj Narantsatsral6Batdorj Davaasuren7Myagmar Zoljargal8Punsantsogvoo Myagmarsuren9Orkhon Banzragchgarav10Noboru Inoue11Naoaki Yokoyama12Jinlin Zhou13Miroslav Bobek14Badgar Battsetseg15Laboratory of Molecular Genetics, Institute of Veterinary Medicine, Ulaanbaatar, Mongolia; Corresponding author at: Institute of Veterinary Medicine, Zaisan, 17024, Ulaanbaatar, Mongolia.Laboratory of Molecular Genetics, Institute of Veterinary Medicine, Ulaanbaatar, Mongolia; Mongolian University of Life Science, Ulaanbaatar, MongoliaLaboratory of Molecular Genetics, Institute of Veterinary Medicine, Ulaanbaatar, Mongolia; National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, JapanLaboratory of Molecular Genetics, Institute of Veterinary Medicine, Ulaanbaatar, MongoliaLaboratory of Molecular Genetics, Institute of Veterinary Medicine, Ulaanbaatar, MongoliaLaboratory of Molecular Genetics, Institute of Veterinary Medicine, Ulaanbaatar, MongoliaLaboratory of Molecular Genetics, Institute of Veterinary Medicine, Ulaanbaatar, MongoliaLaboratory of Molecular Genetics, Institute of Veterinary Medicine, Ulaanbaatar, MongoliaLaboratory of Molecular Genetics, Institute of Veterinary Medicine, Ulaanbaatar, MongoliaLaboratory of Molecular Genetics, Institute of Veterinary Medicine, Ulaanbaatar, MongoliaLaboratory of Molecular Genetics, Institute of Veterinary Medicine, Ulaanbaatar, MongoliaNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan; WOAH Reference Laboratories for Bovine Babesiosis and Equine Piroplasmosis, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, JapanShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, ChinaThe Prague Zoological Garden, Prague, Czech RepublicLaboratory of Molecular Genetics, Institute of Veterinary Medicine, Ulaanbaatar, MongoliaHaemaphysalis (Acari: Ixodidae) ticks remain poorly studied in Mongolia. Here, we report the first confirmed record of Haemaphysalis concinna Koch, 1844 in the Numrug Strictly Protected Area, Dornod Province, eastern Mongolia, based on both morphological characteristics and molecular analyses. This region lies along the Mongolia-China border within the forest-steppe and steppe zones of the western Greater Khingan Mountains. Tick sampling was conducted at six sites (lakeshores, riverbanks, and marshy forests) between early April and mid-August over a three-year period.A total of 1014 H concinna specimens were collected, of which 148 (including two nymphs) were selected for DNA extraction. PCR amplifications targeting the mitochondrial 16S rRNA (∼455 bp) and Cox1 (∼820 bp) genes were performed. Eight samples were sequenced, with three 16S and two Cox1 sequences meeting quality thresholds. The sequences of both genes exhibited 99.8 % and 100 % identity, respectively, to H. concinna sequences from Russia (Cox1: PP851089.1, PP851095.1; 16S rRNA: KP866207.1) and China (Cox1: KR108863.1; 16S rRNA: ON097130.1) available in GenBank. This high level of sequence identity strongly supports the identification of the Mongolian samples as H. concinna. Species identification was further supported by morphological characteristics consistent with published descriptions of adult ticks. This record from the Khalkh Numrug basin represents the first verified occurrence of H. concinna in eastern Mongolia. These findings underscore the need for further research on the species’ ecology, host range, and potential public health significance in the region.http://www.sciencedirect.com/science/article/pii/S1877959X25000858Haemaphysalis concinna, Tick distributionMongolia-china border areaMongolia
spellingShingle Banzragch Battur
Batsaikhan Enkhtaivan
Davaajav Otgonsuren
Batbold Davkharbayar
Dalantai Munkhgerel
Tovuu Amgalanbaatar
Sandagdorj Narantsatsral
Batdorj Davaasuren
Myagmar Zoljargal
Punsantsogvoo Myagmarsuren
Orkhon Banzragchgarav
Noboru Inoue
Naoaki Yokoyama
Jinlin Zhou
Miroslav Bobek
Badgar Battsetseg
First record of Haemaphysalis concinna (Acari: Ixodidae) tick in the Khalkh Numrug basin of Eastern Mongolia
Ticks and Tick-Borne Diseases
Haemaphysalis concinna, Tick distribution
Mongolia-china border area
Mongolia
title First record of Haemaphysalis concinna (Acari: Ixodidae) tick in the Khalkh Numrug basin of Eastern Mongolia
title_full First record of Haemaphysalis concinna (Acari: Ixodidae) tick in the Khalkh Numrug basin of Eastern Mongolia
title_fullStr First record of Haemaphysalis concinna (Acari: Ixodidae) tick in the Khalkh Numrug basin of Eastern Mongolia
title_full_unstemmed First record of Haemaphysalis concinna (Acari: Ixodidae) tick in the Khalkh Numrug basin of Eastern Mongolia
title_short First record of Haemaphysalis concinna (Acari: Ixodidae) tick in the Khalkh Numrug basin of Eastern Mongolia
title_sort first record of haemaphysalis concinna acari ixodidae tick in the khalkh numrug basin of eastern mongolia
topic Haemaphysalis concinna, Tick distribution
Mongolia-china border area
Mongolia
url http://www.sciencedirect.com/science/article/pii/S1877959X25000858
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