GC-MS Analysis and Potential Apoptotic Effect of Paliurus spina-christi Mill. Leaf and Flower Extracts against Breast Cancer Cells
In recent years, herbal medicines have become a significant novel source of treatment for various types of cancer, including breast cancer. Various investigations have declared that Paliurus spina-christi Mill. (PSC) shows antioxidant, antifungal, antimicrobial, and antibacterial properties, but its...
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Sakarya University
2022-04-01
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| Series: | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/2102925 |
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| author | Çisil Çamlı Pulat Ferdi Oguz Harika Atmaca Süleyman İlhan |
| author_facet | Çisil Çamlı Pulat Ferdi Oguz Harika Atmaca Süleyman İlhan |
| author_sort | Çisil Çamlı Pulat |
| collection | DOAJ |
| description | In recent years, herbal medicines have become a significant novel source of treatment for various types of cancer, including breast cancer. Various investigations have declared that Paliurus spina-christi Mill. (PSC) shows antioxidant, antifungal, antimicrobial, and antibacterial properties, but its effect on cancer cells is unknown. This study purposed to evaluate the possible anti-cancer effects of the ethanolic extract of the PSC in human MCF-7 and MDA-MB-231 breast cancer cells. The leaf and flower extracts of PSC were prepared in ethanol and volatile compounds were determined by GC-MS analysis. The possible cytotoxic effects of extracts were evaluated via MTT assay. Apoptotic effect was examined using the PI Annexin V Apoptosis Detection Kit. Significant cytotoxic effects were detected after 72 h treatment of ethanolic leaf and flower extracts in MCF-7 cells but not in MDA-MB-231 cells. Both leaf and flower extracts of PSC induced apoptotic cell death in MCF-7 cells. On phytochemical screening, it was shown that the leaf extract of PSC contains pyrrolidine, 2-decenal, 2-undecanal, phytol, oleic acid, oleamide, squalane, vitamin E, and gamma-sitosterol and the flower extract contains pyrrolidine, 2-decenal, 2-undecenal, oleic acid, lupeol, and gamma-sitosterol. These data report that PSC leaf and flower extracts have cytotoxic and apoptotic effects in MCF-7 breast cancer cells. Moreover, this study can be considered an in vitro background for further in vivo cancer experiments. |
| format | Article |
| id | doaj-art-c37ca5dbd1384cbcb90699e503be9cc4 |
| institution | OA Journals |
| issn | 2147-835X |
| language | English |
| publishDate | 2022-04-01 |
| publisher | Sakarya University |
| record_format | Article |
| series | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
| spelling | doaj-art-c37ca5dbd1384cbcb90699e503be9cc42025-08-20T02:31:52ZengSakarya UniversitySakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi2147-835X2022-04-0126235736410.16984/saufenbilder.102935128GC-MS Analysis and Potential Apoptotic Effect of Paliurus spina-christi Mill. Leaf and Flower Extracts against Breast Cancer CellsÇisil Çamlı Pulat0https://orcid.org/0000-0002-9641-7219Ferdi Oguz1https://orcid.org/0000-0002-3123-8519Harika Atmaca2https://orcid.org/0000-0002-8459-4373Süleyman İlhan3https://orcid.org/0000-0002-6584-3979MANISA CELAL BAYAR UNIVERSITYMANISA CELAL BAYAR UNIVERSITYMANISA CELAL BAYAR UNIVERSITYMANISA CELAL BAYAR UNIVERSITYIn recent years, herbal medicines have become a significant novel source of treatment for various types of cancer, including breast cancer. Various investigations have declared that Paliurus spina-christi Mill. (PSC) shows antioxidant, antifungal, antimicrobial, and antibacterial properties, but its effect on cancer cells is unknown. This study purposed to evaluate the possible anti-cancer effects of the ethanolic extract of the PSC in human MCF-7 and MDA-MB-231 breast cancer cells. The leaf and flower extracts of PSC were prepared in ethanol and volatile compounds were determined by GC-MS analysis. The possible cytotoxic effects of extracts were evaluated via MTT assay. Apoptotic effect was examined using the PI Annexin V Apoptosis Detection Kit. Significant cytotoxic effects were detected after 72 h treatment of ethanolic leaf and flower extracts in MCF-7 cells but not in MDA-MB-231 cells. Both leaf and flower extracts of PSC induced apoptotic cell death in MCF-7 cells. On phytochemical screening, it was shown that the leaf extract of PSC contains pyrrolidine, 2-decenal, 2-undecanal, phytol, oleic acid, oleamide, squalane, vitamin E, and gamma-sitosterol and the flower extract contains pyrrolidine, 2-decenal, 2-undecenal, oleic acid, lupeol, and gamma-sitosterol. These data report that PSC leaf and flower extracts have cytotoxic and apoptotic effects in MCF-7 breast cancer cells. Moreover, this study can be considered an in vitro background for further in vivo cancer experiments.https://dergipark.org.tr/tr/download/article-file/2102925paliurus spina-christibreast canceranti-cancergc-msmcf-7 |
| spellingShingle | Çisil Çamlı Pulat Ferdi Oguz Harika Atmaca Süleyman İlhan GC-MS Analysis and Potential Apoptotic Effect of Paliurus spina-christi Mill. Leaf and Flower Extracts against Breast Cancer Cells Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi paliurus spina-christi breast cancer anti-cancer gc-ms mcf-7 |
| title | GC-MS Analysis and Potential Apoptotic Effect of Paliurus spina-christi Mill. Leaf and Flower Extracts against Breast Cancer Cells |
| title_full | GC-MS Analysis and Potential Apoptotic Effect of Paliurus spina-christi Mill. Leaf and Flower Extracts against Breast Cancer Cells |
| title_fullStr | GC-MS Analysis and Potential Apoptotic Effect of Paliurus spina-christi Mill. Leaf and Flower Extracts against Breast Cancer Cells |
| title_full_unstemmed | GC-MS Analysis and Potential Apoptotic Effect of Paliurus spina-christi Mill. Leaf and Flower Extracts against Breast Cancer Cells |
| title_short | GC-MS Analysis and Potential Apoptotic Effect of Paliurus spina-christi Mill. Leaf and Flower Extracts against Breast Cancer Cells |
| title_sort | gc ms analysis and potential apoptotic effect of paliurus spina christi mill leaf and flower extracts against breast cancer cells |
| topic | paliurus spina-christi breast cancer anti-cancer gc-ms mcf-7 |
| url | https://dergipark.org.tr/tr/download/article-file/2102925 |
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