Effects of different vaccination regimes on the immunodiagnosis of tuberculosis in goats and evaluation of defined antigens
Tuberculosis (TB) in goats is a chronic infectious disease caused by Mycobacterium tuberculosis complex (MTBC) organisms that pose a great health and economic challenge for the caprine industry in some European and developing countries. It is also a zoonotic disease posing a risk for public health....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1524461/full |
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| author | Patricia Cuenca-Lara Patricia Cuenca-Lara Miriam Blay-Benach Miriam Blay-Benach Zoraida Cervera Zoraida Cervera Cristian Melgarejo Cristian Melgarejo Julia Moraleda Julia Moraleda Iker A. Sevilla Joseba M. Garrido Mahavir Singh Gareth J. Jones Bernat Pérez de Val Bernat Pérez de Val |
| author_facet | Patricia Cuenca-Lara Patricia Cuenca-Lara Miriam Blay-Benach Miriam Blay-Benach Zoraida Cervera Zoraida Cervera Cristian Melgarejo Cristian Melgarejo Julia Moraleda Julia Moraleda Iker A. Sevilla Joseba M. Garrido Mahavir Singh Gareth J. Jones Bernat Pérez de Val Bernat Pérez de Val |
| author_sort | Patricia Cuenca-Lara |
| collection | DOAJ |
| description | Tuberculosis (TB) in goats is a chronic infectious disease caused by Mycobacterium tuberculosis complex (MTBC) organisms that pose a great health and economic challenge for the caprine industry in some European and developing countries. It is also a zoonotic disease posing a risk for public health. The control programs of the disease are based on a test-and-slaughter strategy, and vaccination is not feasible with available vaccines due to its interferences with the current TB immunodiagnosis. There is still a need for the development of an effective TB vaccine and, concurrently, diagnostic methods that allow differentiation between infected and vaccinated animals (DIVA approach). In this study, we investigated the interferences caused by the tuberculin (PPD)-based TB diagnostic tests in goats immunized by different mucosal and parenteral vaccination strategies: three single-dose strategies based on intranasal administration of BCG and two heat-inactivated M. bovis (HIMB) vaccines, and two prime-boost strategies based on parenteral BCG or HIMB priming and intranasal HIMB boosting. In addition, the defined antigens ESAT-6, CPF10, and EspC were evaluated as alternative diagnostic reagents to PPDs. At week 14 after prime vaccination of the animals, skin tests, IFN-γ release assay, and antibody detection assays were performed. The two prime-boosted and the single-dose intranasal BCG groups displayed greater cell-mediated immune responses to PPDs than the two single-dose intranasal HIMB vaccines. However, the use of reagents based on the defined antigens eliminated or reduced the vaccine-induced diagnostic interferences in all groups. Based on these results, the use of defined antigens in the current immunodiagnostic tests appears to be suitable in a future goat TB vaccination scenario. |
| format | Article |
| id | doaj-art-c3733dc799204e2bb2ff0c6169604c92 |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-c3733dc799204e2bb2ff0c6169604c922025-01-27T16:54:17ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-01-011110.3389/fvets.2024.15244611524461Effects of different vaccination regimes on the immunodiagnosis of tuberculosis in goats and evaluation of defined antigensPatricia Cuenca-Lara0Patricia Cuenca-Lara1Miriam Blay-Benach2Miriam Blay-Benach3Zoraida Cervera4Zoraida Cervera5Cristian Melgarejo6Cristian Melgarejo7Julia Moraleda8Julia Moraleda9Iker A. Sevilla10Joseba M. Garrido11Mahavir Singh12Gareth J. Jones13Bernat Pérez de Val14Bernat Pérez de Val15Unitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal, Campus de la Universitat Autònoma de Barcelona, Bellaterra, SpainIRTA, Animal Health, Centre de Recerca en Sanitat Animal, Campus de la Universitat Autònoma de Barcelona, Bellaterra, SpainUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal, Campus de la Universitat Autònoma de Barcelona, Bellaterra, SpainIRTA, Animal Health, Centre de Recerca en Sanitat Animal, Campus de la Universitat Autònoma de Barcelona, Bellaterra, SpainUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal, Campus de la Universitat Autònoma de Barcelona, Bellaterra, SpainIRTA, Animal Health, Centre de Recerca en Sanitat Animal, Campus de la Universitat Autònoma de Barcelona, Bellaterra, SpainUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal, Campus de la Universitat Autònoma de Barcelona, Bellaterra, SpainIRTA, Animal Health, Centre de Recerca en Sanitat Animal, Campus de la Universitat Autònoma de Barcelona, Bellaterra, SpainUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal, Campus de la Universitat Autònoma de Barcelona, Bellaterra, SpainIRTA, Animal Health, Centre de Recerca en Sanitat Animal, Campus de la Universitat Autònoma de Barcelona, Bellaterra, SpainAnimal Health Department, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Basque Research and Technology Alliance, Derio, SpainAnimal Health Department, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Basque Research and Technology Alliance, Derio, SpainLionex Diagnostics and Therapeutics GmbH, Braunschweig, GermanyDepartment of Bacteriology, Animal and Plant Health Agency, Addlestone, United KingdomUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal, Campus de la Universitat Autònoma de Barcelona, Bellaterra, SpainIRTA, Animal Health, Centre de Recerca en Sanitat Animal, Campus de la Universitat Autònoma de Barcelona, Bellaterra, SpainTuberculosis (TB) in goats is a chronic infectious disease caused by Mycobacterium tuberculosis complex (MTBC) organisms that pose a great health and economic challenge for the caprine industry in some European and developing countries. It is also a zoonotic disease posing a risk for public health. The control programs of the disease are based on a test-and-slaughter strategy, and vaccination is not feasible with available vaccines due to its interferences with the current TB immunodiagnosis. There is still a need for the development of an effective TB vaccine and, concurrently, diagnostic methods that allow differentiation between infected and vaccinated animals (DIVA approach). In this study, we investigated the interferences caused by the tuberculin (PPD)-based TB diagnostic tests in goats immunized by different mucosal and parenteral vaccination strategies: three single-dose strategies based on intranasal administration of BCG and two heat-inactivated M. bovis (HIMB) vaccines, and two prime-boost strategies based on parenteral BCG or HIMB priming and intranasal HIMB boosting. In addition, the defined antigens ESAT-6, CPF10, and EspC were evaluated as alternative diagnostic reagents to PPDs. At week 14 after prime vaccination of the animals, skin tests, IFN-γ release assay, and antibody detection assays were performed. The two prime-boosted and the single-dose intranasal BCG groups displayed greater cell-mediated immune responses to PPDs than the two single-dose intranasal HIMB vaccines. However, the use of reagents based on the defined antigens eliminated or reduced the vaccine-induced diagnostic interferences in all groups. Based on these results, the use of defined antigens in the current immunodiagnostic tests appears to be suitable in a future goat TB vaccination scenario.https://www.frontiersin.org/articles/10.3389/fvets.2024.1524461/fullvaccinesMycobacterium bovistuberculosisdiagnosisskin testIGRA |
| spellingShingle | Patricia Cuenca-Lara Patricia Cuenca-Lara Miriam Blay-Benach Miriam Blay-Benach Zoraida Cervera Zoraida Cervera Cristian Melgarejo Cristian Melgarejo Julia Moraleda Julia Moraleda Iker A. Sevilla Joseba M. Garrido Mahavir Singh Gareth J. Jones Bernat Pérez de Val Bernat Pérez de Val Effects of different vaccination regimes on the immunodiagnosis of tuberculosis in goats and evaluation of defined antigens Frontiers in Veterinary Science vaccines Mycobacterium bovis tuberculosis diagnosis skin test IGRA |
| title | Effects of different vaccination regimes on the immunodiagnosis of tuberculosis in goats and evaluation of defined antigens |
| title_full | Effects of different vaccination regimes on the immunodiagnosis of tuberculosis in goats and evaluation of defined antigens |
| title_fullStr | Effects of different vaccination regimes on the immunodiagnosis of tuberculosis in goats and evaluation of defined antigens |
| title_full_unstemmed | Effects of different vaccination regimes on the immunodiagnosis of tuberculosis in goats and evaluation of defined antigens |
| title_short | Effects of different vaccination regimes on the immunodiagnosis of tuberculosis in goats and evaluation of defined antigens |
| title_sort | effects of different vaccination regimes on the immunodiagnosis of tuberculosis in goats and evaluation of defined antigens |
| topic | vaccines Mycobacterium bovis tuberculosis diagnosis skin test IGRA |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1524461/full |
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