Process Optimization of Thawed Cloudy Huyou Juice Clarification Using a Composite of Carboxymethyl Chitosan and Sodium Alginate

Cloudy huyou juice is increasingly popular for its unique flavor, but flocculent precipitation after cold storage and thawing affects its sensory quality and increases production costs. This study optimized the clarification of thawed cloudy huyou juice using a composite of carboxymethyl chitosan (C...

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Main Authors: Peichao Zhang, Liang Zhang, Xiayu Liu, Yuxi Wang, Jiatong Xu, Pengfei Liu, Boyuan Guan
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/14/15/2658
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author Peichao Zhang
Liang Zhang
Xiayu Liu
Yuxi Wang
Jiatong Xu
Pengfei Liu
Boyuan Guan
author_facet Peichao Zhang
Liang Zhang
Xiayu Liu
Yuxi Wang
Jiatong Xu
Pengfei Liu
Boyuan Guan
author_sort Peichao Zhang
collection DOAJ
description Cloudy huyou juice is increasingly popular for its unique flavor, but flocculent precipitation after cold storage and thawing affects its sensory quality and increases production costs. This study optimized the clarification of thawed cloudy huyou juice using a composite of carboxymethyl chitosan (CC) and sodium alginate (SA), prepared via ionic and covalent crosslinking. The composite was characterized by SEM, FTIR, and thermal analysis. Transmittance was used to evaluate clarification performance. The effects of dosage, adsorption time, and temperature were first assessed through single-factor experiments, followed by optimization using a Box–Behnken response surface methodology. The composite significantly improved clarity (<i>p</i> < 0.05), reaching 85.38% transmittance under optimal conditions: 22 mg dosage, 80 min time, and 38 °C. The composite dosage and temperature were the most influential factors. Reusability tests showed declining performance, with the transmittance dropping to 57.13% after five cycles, likely due to incomplete desorption of adsorbed compounds. These results suggest that the CC-SA composite is an effective and reusable clarifying agent with potential for industrial applications in turbid fruit juice processing.
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publisher MDPI AG
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series Foods
spelling doaj-art-c36f34c863f745cb907e6f29df57d27d2025-08-20T03:36:31ZengMDPI AGFoods2304-81582025-07-011415265810.3390/foods14152658Process Optimization of Thawed Cloudy Huyou Juice Clarification Using a Composite of Carboxymethyl Chitosan and Sodium AlginatePeichao Zhang0Liang Zhang1Xiayu Liu2Yuxi Wang3Jiatong Xu4Pengfei Liu5Boyuan Guan6College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaInnovation Center of Yangtze River Delta, Zhejiang University, Jiaxing 314100, ChinaInnovation Center of Yangtze River Delta, Zhejiang University, Jiaxing 314100, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaCloudy huyou juice is increasingly popular for its unique flavor, but flocculent precipitation after cold storage and thawing affects its sensory quality and increases production costs. This study optimized the clarification of thawed cloudy huyou juice using a composite of carboxymethyl chitosan (CC) and sodium alginate (SA), prepared via ionic and covalent crosslinking. The composite was characterized by SEM, FTIR, and thermal analysis. Transmittance was used to evaluate clarification performance. The effects of dosage, adsorption time, and temperature were first assessed through single-factor experiments, followed by optimization using a Box–Behnken response surface methodology. The composite significantly improved clarity (<i>p</i> < 0.05), reaching 85.38% transmittance under optimal conditions: 22 mg dosage, 80 min time, and 38 °C. The composite dosage and temperature were the most influential factors. Reusability tests showed declining performance, with the transmittance dropping to 57.13% after five cycles, likely due to incomplete desorption of adsorbed compounds. These results suggest that the CC-SA composite is an effective and reusable clarifying agent with potential for industrial applications in turbid fruit juice processing.https://www.mdpi.com/2304-8158/14/15/2658huyou juiceclarificationcarboxymethyl chitosansodium alginateresponse surface methodology
spellingShingle Peichao Zhang
Liang Zhang
Xiayu Liu
Yuxi Wang
Jiatong Xu
Pengfei Liu
Boyuan Guan
Process Optimization of Thawed Cloudy Huyou Juice Clarification Using a Composite of Carboxymethyl Chitosan and Sodium Alginate
Foods
huyou juice
clarification
carboxymethyl chitosan
sodium alginate
response surface methodology
title Process Optimization of Thawed Cloudy Huyou Juice Clarification Using a Composite of Carboxymethyl Chitosan and Sodium Alginate
title_full Process Optimization of Thawed Cloudy Huyou Juice Clarification Using a Composite of Carboxymethyl Chitosan and Sodium Alginate
title_fullStr Process Optimization of Thawed Cloudy Huyou Juice Clarification Using a Composite of Carboxymethyl Chitosan and Sodium Alginate
title_full_unstemmed Process Optimization of Thawed Cloudy Huyou Juice Clarification Using a Composite of Carboxymethyl Chitosan and Sodium Alginate
title_short Process Optimization of Thawed Cloudy Huyou Juice Clarification Using a Composite of Carboxymethyl Chitosan and Sodium Alginate
title_sort process optimization of thawed cloudy huyou juice clarification using a composite of carboxymethyl chitosan and sodium alginate
topic huyou juice
clarification
carboxymethyl chitosan
sodium alginate
response surface methodology
url https://www.mdpi.com/2304-8158/14/15/2658
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