Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny.
The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonst...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0051361&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850224563949928448 |
|---|---|
| author | Simon D J Calaminus Amelie V Guitart Amy Sinclair Hannah Schachtner Steve P Watson Tessa L Holyoake Kamil R Kranc Laura M Machesky |
| author_facet | Simon D J Calaminus Amelie V Guitart Amy Sinclair Hannah Schachtner Steve P Watson Tessa L Holyoake Kamil R Kranc Laura M Machesky |
| author_sort | Simon D J Calaminus |
| collection | DOAJ |
| description | The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15-60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage. |
| format | Article |
| id | doaj-art-c36bdc75aa094263aeb4f2c1eb954105 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-c36bdc75aa094263aeb4f2c1eb9541052025-08-20T02:05:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5136110.1371/journal.pone.0051361Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny.Simon D J CalaminusAmelie V GuitartAmy SinclairHannah SchachtnerSteve P WatsonTessa L HolyoakeKamil R KrancLaura M MacheskyThe development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15-60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0051361&type=printable |
| spellingShingle | Simon D J Calaminus Amelie V Guitart Amy Sinclair Hannah Schachtner Steve P Watson Tessa L Holyoake Kamil R Kranc Laura M Machesky Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. PLoS ONE |
| title | Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. |
| title_full | Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. |
| title_fullStr | Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. |
| title_full_unstemmed | Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. |
| title_short | Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. |
| title_sort | lineage tracing of pf4 cre marks hematopoietic stem cells and their progeny |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0051361&type=printable |
| work_keys_str_mv | AT simondjcalaminus lineagetracingofpf4cremarkshematopoieticstemcellsandtheirprogeny AT amelievguitart lineagetracingofpf4cremarkshematopoieticstemcellsandtheirprogeny AT amysinclair lineagetracingofpf4cremarkshematopoieticstemcellsandtheirprogeny AT hannahschachtner lineagetracingofpf4cremarkshematopoieticstemcellsandtheirprogeny AT stevepwatson lineagetracingofpf4cremarkshematopoieticstemcellsandtheirprogeny AT tessalholyoake lineagetracingofpf4cremarkshematopoieticstemcellsandtheirprogeny AT kamilrkranc lineagetracingofpf4cremarkshematopoieticstemcellsandtheirprogeny AT laurammachesky lineagetracingofpf4cremarkshematopoieticstemcellsandtheirprogeny |