Hydrogen Sulfide Expression May Affect Ovarian Cancer Progression and Drug Resistance

Qiong Zhu,1,2 Yi Liu,3 Juan Zhou,4 Miduo Tan,2,5 Yaxin Xue,6 Yin Tao2,7 1Department of Gynaecology, Zhuzhou Central Hospital, Zhuzhou, 412000, People’s Republic of China; 2Department of Central Laboratory, Zhuzhou Central Hospital, Zhuzhou, 412000, People’s Republic of China; 3Department of Transfus...

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Main Authors: Zhu Q, Liu Y, Zhou J, Tan M, Xue Y, Tao Y
Format: Article
Language:English
Published: Dove Medical Press 2025-05-01
Series:International Journal of Women's Health
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Online Access:https://www.dovepress.com/hydrogen-sulfide-expression-may-affect-ovarian-cancer-progression-and--peer-reviewed-fulltext-article-IJWH
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author Zhu Q
Liu Y
Zhou J
Tan M
Xue Y
Tao Y
author_facet Zhu Q
Liu Y
Zhou J
Tan M
Xue Y
Tao Y
author_sort Zhu Q
collection DOAJ
description Qiong Zhu,1,2 Yi Liu,3 Juan Zhou,4 Miduo Tan,2,5 Yaxin Xue,6 Yin Tao2,7 1Department of Gynaecology, Zhuzhou Central Hospital, Zhuzhou, 412000, People’s Republic of China; 2Department of Central Laboratory, Zhuzhou Central Hospital, Zhuzhou, 412000, People’s Republic of China; 3Department of Transfusion, Zhuzhou Central Hospital, Zhuzhou, 412000, People’s Republic of China; 4Department Respiratory and Critical Care Medicine, Zhuzhou Central Hospital, Zhuzhou, 412000, People’s Republic of China; 5Department of Gastroenterology Medical Center and Thyroid Gastrointestinal Hernia Surgery, Digestive Disease Medical Center, Zhuzhou Central Hospital, Zhuzhou, 412000, People’s Republic of China; 6Department of Cardiology, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University, Zhuzhou, 412000, People’s Republic of China; 7Department of General Surgery, Zhuzhou Central Hospital, Zhuzhou, 412000, People’s Republic of ChinaCorrespondence: Yaxin Xue, Department of Cardiology, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University, Zhuzhou, 412000, People’s Republic of China, Email smile2992smile@163.com Yin Tao, Department of General Surgery, Zhuzhou Central Hospital, Zhuzhou, 412000, People’s Republic of China, Email 340468999@qq.comObjective: The present study aimed to evaluated the expression profiles of the Hydrogen sulfide (H2S)-synthesizing enzymes cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) expression and attempted to establish its relationship to ovarian cancer (OV) drug resistance and prognosis.Methods: The expression levels of cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) were analyzed in OV from GSE26193 and GSE14764 datasets, and OVCAR8 cisplatin resistant cell line from GSE45553 datasets. The correlation between prognosis with CBS and CSE expression levels were analyzed in OV from GSE26193 dataset. Immunohistochemical (IHC) analysis of CBS and CSE expression, meanwhile Kaplan-Meier analyzed of corresponding prognosis in OV patients. The relative drug sensitivity of CBS and CSE genes via gene set cancer analysis. Ultimately, the correlations among OV immune cells, CBS, and CSE. The CBS and CSE expression levels, and pan-cancer prognosis using TCGA database.Results: CBS and CSE were significantly overexpressed in the OV and OVCAR8 cisplatin resistant cell line (GSE26193: p < 0.001 and p < 0.0001; GSE14764: p < 0.001 and p < 0.0001 IHC: p< 0.0001 and p< 0.0001; GSE45553: p < 0.0001 and p < 0.0001, respectively). Interestingly, CBS is closely associated with OS and PFS in GSE26193 dataset and OV patients (GSE26193: PFS, p = 0.0391; OS, p = 0.0328. OV patients: PFS, p= 0.017; OS, p=0.030). The CBS and CSE expression levels were correlated with immune cells, CD4 T cells, neutrophils, macrophages, and dendritic cells (DC) and their respective infiltration scores significantly differed. Both CBS and CSE expression levels influenced pan-cancer prognosis.Conclusion: H2S overexpression affected the OS and PFS and could also have an impact on the drug resistance of OV. Pan cancer prognosis may vary with H2S expression.Keywords: drug resistance, hydrogen sulfide, immune cell, ovarian cancer, pan-cancer, progression
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spelling doaj-art-c35f8b2e73694756bf00dde76f7a86e02025-08-20T03:32:36ZengDove Medical PressInternational Journal of Women's Health1179-14112025-05-01Volume 17Issue 115331545103361Hydrogen Sulfide Expression May Affect Ovarian Cancer Progression and Drug ResistanceZhu Q0Liu Y1Zhou JTan M2Xue YTao Y3Department of GynaecologyDepartment of TransfusionDepartment of Breast Surgery,Department of General SurgeryQiong Zhu,1,2 Yi Liu,3 Juan Zhou,4 Miduo Tan,2,5 Yaxin Xue,6 Yin Tao2,7 1Department of Gynaecology, Zhuzhou Central Hospital, Zhuzhou, 412000, People’s Republic of China; 2Department of Central Laboratory, Zhuzhou Central Hospital, Zhuzhou, 412000, People’s Republic of China; 3Department of Transfusion, Zhuzhou Central Hospital, Zhuzhou, 412000, People’s Republic of China; 4Department Respiratory and Critical Care Medicine, Zhuzhou Central Hospital, Zhuzhou, 412000, People’s Republic of China; 5Department of Gastroenterology Medical Center and Thyroid Gastrointestinal Hernia Surgery, Digestive Disease Medical Center, Zhuzhou Central Hospital, Zhuzhou, 412000, People’s Republic of China; 6Department of Cardiology, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University, Zhuzhou, 412000, People’s Republic of China; 7Department of General Surgery, Zhuzhou Central Hospital, Zhuzhou, 412000, People’s Republic of ChinaCorrespondence: Yaxin Xue, Department of Cardiology, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University, Zhuzhou, 412000, People’s Republic of China, Email smile2992smile@163.com Yin Tao, Department of General Surgery, Zhuzhou Central Hospital, Zhuzhou, 412000, People’s Republic of China, Email 340468999@qq.comObjective: The present study aimed to evaluated the expression profiles of the Hydrogen sulfide (H2S)-synthesizing enzymes cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) expression and attempted to establish its relationship to ovarian cancer (OV) drug resistance and prognosis.Methods: The expression levels of cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) were analyzed in OV from GSE26193 and GSE14764 datasets, and OVCAR8 cisplatin resistant cell line from GSE45553 datasets. The correlation between prognosis with CBS and CSE expression levels were analyzed in OV from GSE26193 dataset. Immunohistochemical (IHC) analysis of CBS and CSE expression, meanwhile Kaplan-Meier analyzed of corresponding prognosis in OV patients. The relative drug sensitivity of CBS and CSE genes via gene set cancer analysis. Ultimately, the correlations among OV immune cells, CBS, and CSE. The CBS and CSE expression levels, and pan-cancer prognosis using TCGA database.Results: CBS and CSE were significantly overexpressed in the OV and OVCAR8 cisplatin resistant cell line (GSE26193: p < 0.001 and p < 0.0001; GSE14764: p < 0.001 and p < 0.0001 IHC: p< 0.0001 and p< 0.0001; GSE45553: p < 0.0001 and p < 0.0001, respectively). Interestingly, CBS is closely associated with OS and PFS in GSE26193 dataset and OV patients (GSE26193: PFS, p = 0.0391; OS, p = 0.0328. OV patients: PFS, p= 0.017; OS, p=0.030). The CBS and CSE expression levels were correlated with immune cells, CD4 T cells, neutrophils, macrophages, and dendritic cells (DC) and their respective infiltration scores significantly differed. Both CBS and CSE expression levels influenced pan-cancer prognosis.Conclusion: H2S overexpression affected the OS and PFS and could also have an impact on the drug resistance of OV. Pan cancer prognosis may vary with H2S expression.Keywords: drug resistance, hydrogen sulfide, immune cell, ovarian cancer, pan-cancer, progressionhttps://www.dovepress.com/hydrogen-sulfide-expression-may-affect-ovarian-cancer-progression-and--peer-reviewed-fulltext-article-IJWHDrug resistanceHydrogen sulfideImmune cellOvarian cancerPan-cancerProgression
spellingShingle Zhu Q
Liu Y
Zhou J
Tan M
Xue Y
Tao Y
Hydrogen Sulfide Expression May Affect Ovarian Cancer Progression and Drug Resistance
International Journal of Women's Health
Drug resistance
Hydrogen sulfide
Immune cell
Ovarian cancer
Pan-cancer
Progression
title Hydrogen Sulfide Expression May Affect Ovarian Cancer Progression and Drug Resistance
title_full Hydrogen Sulfide Expression May Affect Ovarian Cancer Progression and Drug Resistance
title_fullStr Hydrogen Sulfide Expression May Affect Ovarian Cancer Progression and Drug Resistance
title_full_unstemmed Hydrogen Sulfide Expression May Affect Ovarian Cancer Progression and Drug Resistance
title_short Hydrogen Sulfide Expression May Affect Ovarian Cancer Progression and Drug Resistance
title_sort hydrogen sulfide expression may affect ovarian cancer progression and drug resistance
topic Drug resistance
Hydrogen sulfide
Immune cell
Ovarian cancer
Pan-cancer
Progression
url https://www.dovepress.com/hydrogen-sulfide-expression-may-affect-ovarian-cancer-progression-and--peer-reviewed-fulltext-article-IJWH
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AT liuy hydrogensulfideexpressionmayaffectovariancancerprogressionanddrugresistance
AT zhouj hydrogensulfideexpressionmayaffectovariancancerprogressionanddrugresistance
AT tanm hydrogensulfideexpressionmayaffectovariancancerprogressionanddrugresistance
AT xuey hydrogensulfideexpressionmayaffectovariancancerprogressionanddrugresistance
AT taoy hydrogensulfideexpressionmayaffectovariancancerprogressionanddrugresistance