Production of Multiple Hydrolytic Enzymes by Black Aspergilli Isolated from Date and Grape

including cellulase, tannase and pectinase. Isolates were morphologically and molecularly identified as Aspergillus niger, Aspergillus tubingensis, Aspergillus japonicus and Aspergillus aculeatus. Isolates were screened for enzyme production ability on solid and in liquid media. Enzymatic activity w...

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Main Authors: Zehra Gulsunoglu, Meral Kilic-akyilmaz, Funda Karbancioglu-guler, Katleen Raes
Format: Article
Language:English
Published: Ankara University 2019-12-01
Series:Journal of Agricultural Sciences
Subjects:
Online Access:https://dergipark.org.tr/tr/download/article-file/871241
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author Zehra Gulsunoglu
Meral Kilic-akyilmaz
Funda Karbancioglu-guler
Katleen Raes
author_facet Zehra Gulsunoglu
Meral Kilic-akyilmaz
Funda Karbancioglu-guler
Katleen Raes
author_sort Zehra Gulsunoglu
collection DOAJ
description including cellulase, tannase and pectinase. Isolates were morphologically and molecularly identified as Aspergillus niger, Aspergillus tubingensis, Aspergillus japonicus and Aspergillus aculeatus. Isolates were screened for enzyme production ability on solid and in liquid media. Enzymatic activity was determined in the culture filtrate of liquid medium. A total of six isolates were found to produce multiple hydrolytic enzymes. The highest activity of cellulase was produced by A. japonicus ZGM4 and A. aculeatus ZGM6 as 40 and 35 U g-1 dry biomass, respectively. All the isolates exhibited high level of tannase activity in the range of 150-343 U g-1 dry biomass after 24 h of incubation. A. tubingensis ZGM5 and A. aculeatus ZGM6 were found to produce the highest pectinase activity at a level of 130 and 117 U g-1 dry biomass, respectively. In the light of these results, isolates can be used for multiple hydrolytic enzyme production in industry.
format Article
id doaj-art-c35e37facdc44b55ae536bd711e42bdf
institution DOAJ
issn 1300-7580
2148-9297
language English
publishDate 2019-12-01
publisher Ankara University
record_format Article
series Journal of Agricultural Sciences
spelling doaj-art-c35e37facdc44b55ae536bd711e42bdf2025-08-20T03:04:58ZengAnkara UniversityJournal of Agricultural Sciences1300-75802148-92972019-12-0125445946610.15832/ankutbd.44826445Production of Multiple Hydrolytic Enzymes by Black Aspergilli Isolated from Date and GrapeZehra Gulsunoglu0Meral Kilic-akyilmaz1Funda Karbancioglu-guler2Katleen Raes3İSTANBUL TEKNİK ÜNİVERSİTESİİSTANBUL TEKNİK ÜNİVERSİTESİİSTANBUL TEKNİK ÜNİVERSİTESİGhent Universityincluding cellulase, tannase and pectinase. Isolates were morphologically and molecularly identified as Aspergillus niger, Aspergillus tubingensis, Aspergillus japonicus and Aspergillus aculeatus. Isolates were screened for enzyme production ability on solid and in liquid media. Enzymatic activity was determined in the culture filtrate of liquid medium. A total of six isolates were found to produce multiple hydrolytic enzymes. The highest activity of cellulase was produced by A. japonicus ZGM4 and A. aculeatus ZGM6 as 40 and 35 U g-1 dry biomass, respectively. All the isolates exhibited high level of tannase activity in the range of 150-343 U g-1 dry biomass after 24 h of incubation. A. tubingensis ZGM5 and A. aculeatus ZGM6 were found to produce the highest pectinase activity at a level of 130 and 117 U g-1 dry biomass, respectively. In the light of these results, isolates can be used for multiple hydrolytic enzyme production in industry.https://dergipark.org.tr/tr/download/article-file/871241aspergilluscellulasepectinasetannase
spellingShingle Zehra Gulsunoglu
Meral Kilic-akyilmaz
Funda Karbancioglu-guler
Katleen Raes
Production of Multiple Hydrolytic Enzymes by Black Aspergilli Isolated from Date and Grape
Journal of Agricultural Sciences
aspergillus
cellulase
pectinase
tannase
title Production of Multiple Hydrolytic Enzymes by Black Aspergilli Isolated from Date and Grape
title_full Production of Multiple Hydrolytic Enzymes by Black Aspergilli Isolated from Date and Grape
title_fullStr Production of Multiple Hydrolytic Enzymes by Black Aspergilli Isolated from Date and Grape
title_full_unstemmed Production of Multiple Hydrolytic Enzymes by Black Aspergilli Isolated from Date and Grape
title_short Production of Multiple Hydrolytic Enzymes by Black Aspergilli Isolated from Date and Grape
title_sort production of multiple hydrolytic enzymes by black aspergilli isolated from date and grape
topic aspergillus
cellulase
pectinase
tannase
url https://dergipark.org.tr/tr/download/article-file/871241
work_keys_str_mv AT zehragulsunoglu productionofmultiplehydrolyticenzymesbyblackaspergilliisolatedfromdateandgrape
AT meralkilicakyilmaz productionofmultiplehydrolyticenzymesbyblackaspergilliisolatedfromdateandgrape
AT fundakarbanciogluguler productionofmultiplehydrolyticenzymesbyblackaspergilliisolatedfromdateandgrape
AT katleenraes productionofmultiplehydrolyticenzymesbyblackaspergilliisolatedfromdateandgrape