Alteration of Inflammatory Mediators in the Upper and Lower Airways under Chronic Intermittent Hypoxia: Preliminary Animal Study
Purpose. We hypothesized that CIH may affect the upper airway immune system and aimed to verify whether CIH can induce airway inflammation in a murine obstructive sleep apnea (OSA) model. Methods. C57BL6 male mice were exposed to intermittent hypoxia (CIH group; 5 ~ 21% FiO2, 120 sec cycles, 12 h/d,...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/4327237 |
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| author | Eun Jung Lee Woon Heo Joo Young Kim Hyungchul Kim Min Jung Kang Bo Ra Kim Ji Hyun Kim Do Yang Park Chang-Hoon Kim Joo-Heon Yoon Hyung-Ju Cho |
| author_facet | Eun Jung Lee Woon Heo Joo Young Kim Hyungchul Kim Min Jung Kang Bo Ra Kim Ji Hyun Kim Do Yang Park Chang-Hoon Kim Joo-Heon Yoon Hyung-Ju Cho |
| author_sort | Eun Jung Lee |
| collection | DOAJ |
| description | Purpose. We hypothesized that CIH may affect the upper airway immune system and aimed to verify whether CIH can induce airway inflammation in a murine obstructive sleep apnea (OSA) model. Methods. C57BL6 male mice were exposed to intermittent hypoxia (CIH group; 5 ~ 21% FiO2, 120 sec cycles, 12 h/d, n=6) or room air (Sham group, n=6) for up to 4 weeks in identical chambers. Nasal and lung tissues and lavage fluid were collected and analyzed by multiplex assay. Lung lavage fluid was also utilized for FACS analysis to determine eosinophil count. Results. We determined the protein level of 24 different cytokines, chemokines, and inflammatory mediators. Among various cytokines, levels of IL-1α, IL-1β, IL-4, IL-6, and IL-13 were significantly elevated in nose or lung tissue from the CIH group. In addition, MCP-1 and periostin were elevated in nose and lung tissue and lavage fluid from the CIH group. Conclusions. CIH for 4 weeks altered the levels of inflammatory mediators in both the nose and lungs of mouse model. We suggest that the airway immune system may be deteriorated by CIH and allergic inflammation in the upper or lower airway could be worsened by sleep apnea. |
| format | Article |
| id | doaj-art-c346ee418d104dc3b6baeb4bcf5c8548 |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-c346ee418d104dc3b6baeb4bcf5c85482025-08-20T03:23:12ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/43272374327237Alteration of Inflammatory Mediators in the Upper and Lower Airways under Chronic Intermittent Hypoxia: Preliminary Animal StudyEun Jung Lee0Woon Heo1Joo Young Kim2Hyungchul Kim3Min Jung Kang4Bo Ra Kim5Ji Hyun Kim6Do Yang Park7Chang-Hoon Kim8Joo-Heon Yoon9Hyung-Ju Cho10Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Republic of KoreaDepartment of Pharmacology, Yonsei University College of Medicine, Seoul, Republic of KoreaDepartment of Pharmacology, Yonsei University College of Medicine, Seoul, Republic of KoreaDepartment of Chemical and Biomolecular Engineering, Sogang University, Seoul, Republic of KoreaThe Research Center for Human Natural Defense System, Yonsei University College of Medicine, Seoul, Republic of KoreaThe Research Center for Human Natural Defense System, Yonsei University College of Medicine, Seoul, Republic of KoreaThe Research Center for Human Natural Defense System, Yonsei University College of Medicine, Seoul, Republic of KoreaDepartment of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Republic of KoreaDepartment of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Republic of KoreaDepartment of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Republic of KoreaDepartment of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Republic of KoreaPurpose. We hypothesized that CIH may affect the upper airway immune system and aimed to verify whether CIH can induce airway inflammation in a murine obstructive sleep apnea (OSA) model. Methods. C57BL6 male mice were exposed to intermittent hypoxia (CIH group; 5 ~ 21% FiO2, 120 sec cycles, 12 h/d, n=6) or room air (Sham group, n=6) for up to 4 weeks in identical chambers. Nasal and lung tissues and lavage fluid were collected and analyzed by multiplex assay. Lung lavage fluid was also utilized for FACS analysis to determine eosinophil count. Results. We determined the protein level of 24 different cytokines, chemokines, and inflammatory mediators. Among various cytokines, levels of IL-1α, IL-1β, IL-4, IL-6, and IL-13 were significantly elevated in nose or lung tissue from the CIH group. In addition, MCP-1 and periostin were elevated in nose and lung tissue and lavage fluid from the CIH group. Conclusions. CIH for 4 weeks altered the levels of inflammatory mediators in both the nose and lungs of mouse model. We suggest that the airway immune system may be deteriorated by CIH and allergic inflammation in the upper or lower airway could be worsened by sleep apnea.http://dx.doi.org/10.1155/2017/4327237 |
| spellingShingle | Eun Jung Lee Woon Heo Joo Young Kim Hyungchul Kim Min Jung Kang Bo Ra Kim Ji Hyun Kim Do Yang Park Chang-Hoon Kim Joo-Heon Yoon Hyung-Ju Cho Alteration of Inflammatory Mediators in the Upper and Lower Airways under Chronic Intermittent Hypoxia: Preliminary Animal Study Mediators of Inflammation |
| title | Alteration of Inflammatory Mediators in the Upper and Lower Airways under Chronic Intermittent Hypoxia: Preliminary Animal Study |
| title_full | Alteration of Inflammatory Mediators in the Upper and Lower Airways under Chronic Intermittent Hypoxia: Preliminary Animal Study |
| title_fullStr | Alteration of Inflammatory Mediators in the Upper and Lower Airways under Chronic Intermittent Hypoxia: Preliminary Animal Study |
| title_full_unstemmed | Alteration of Inflammatory Mediators in the Upper and Lower Airways under Chronic Intermittent Hypoxia: Preliminary Animal Study |
| title_short | Alteration of Inflammatory Mediators in the Upper and Lower Airways under Chronic Intermittent Hypoxia: Preliminary Animal Study |
| title_sort | alteration of inflammatory mediators in the upper and lower airways under chronic intermittent hypoxia preliminary animal study |
| url | http://dx.doi.org/10.1155/2017/4327237 |
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