Antimicrobial efficacy of blueM gel and calcium hydroxide against F. nucleatum and E. faecalis biofilm: an ex-vivo study

Abstract Endodontic treatment failure can result from persistent bacteria, inadequate cleaning and sealing of root canals, and missed canals. The resilience of bacteria such as F. nucleatum and E. faecalis within the root canal system is a significant cause of failed endodontic treatment. Calcium hy...

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Main Authors: Yahya Raja Alharbi, Swati Srivastava, Hanin Alsalhi, Khawlah Almohimeed, Mohammad Alduraibi, Ahmed Nabil
Format: Article
Language:English
Published: Springer 2025-07-01
Series:Saudi Dental Journal
Subjects:
Online Access:https://doi.org/10.1007/s44445-025-00042-x
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author Yahya Raja Alharbi
Swati Srivastava
Hanin Alsalhi
Khawlah Almohimeed
Mohammad Alduraibi
Ahmed Nabil
author_facet Yahya Raja Alharbi
Swati Srivastava
Hanin Alsalhi
Khawlah Almohimeed
Mohammad Alduraibi
Ahmed Nabil
author_sort Yahya Raja Alharbi
collection DOAJ
description Abstract Endodontic treatment failure can result from persistent bacteria, inadequate cleaning and sealing of root canals, and missed canals. The resilience of bacteria such as F. nucleatum and E. faecalis within the root canal system is a significant cause of failed endodontic treatment. Calcium hydroxide (CaOH), a widely utilized intracanal medicament, has demonstrated antimicrobial properties. However, its ability to consistently achieve culture-negative root canals after chemo-mechanical procedures remains inconsistent. This study aimed to evaluate and compare the antimicrobial efficacy of CaOH and BlueM gel against F. nucleatum and E. faecalis biofilms using dentin discs. Sterilized dentin discs were prepared from extracted human teeth. F. nucleatum and E. faecalis were grown on these discs and then treated with either CaOH or BlueM gel. Antimicrobial efficacy was assessed by quantifying colony-forming unit counts. BlueM gel demonstrated significantly greater antimicrobial efficacy than CaOH against both F. nucleatum and E. faecalis biofilms. Both agents exhibited substantial antimicrobial activity against the biofilms. BlueM gel showed superior antimicrobial effectiveness against endodontic pathogens such as F. nucleatum and E. faecalis compared to CaOH.
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institution Kabale University
issn 1013-9052
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publishDate 2025-07-01
publisher Springer
record_format Article
series Saudi Dental Journal
spelling doaj-art-c343ff7ee6d24c46ac2497aab2e2a5ec2025-08-20T04:02:56ZengSpringerSaudi Dental Journal1013-90522524-17022025-07-01377-91710.1007/s44445-025-00042-xAntimicrobial efficacy of blueM gel and calcium hydroxide against F. nucleatum and E. faecalis biofilm: an ex-vivo studyYahya Raja Alharbi0Swati Srivastava1Hanin Alsalhi2Khawlah Almohimeed3Mohammad Alduraibi4Ahmed Nabil5Department of Conservative Dental Sciences, College of Dentistry, Qassim UniversityDepartment of Conservative Dental Sciences, College of Dentistry, Qassim UniversityDepartment of Conservative Dental Sciences, College of Dentistry, Qassim UniversityDepartment of Conservative Dental Sciences, College of Dentistry, Qassim UniversityDepartment of Conservative Dental Sciences, College of Dentistry, Qassim UniversityDepartment of Conservative Dental Sciences, College of Dentistry, Qassim UniversityAbstract Endodontic treatment failure can result from persistent bacteria, inadequate cleaning and sealing of root canals, and missed canals. The resilience of bacteria such as F. nucleatum and E. faecalis within the root canal system is a significant cause of failed endodontic treatment. Calcium hydroxide (CaOH), a widely utilized intracanal medicament, has demonstrated antimicrobial properties. However, its ability to consistently achieve culture-negative root canals after chemo-mechanical procedures remains inconsistent. This study aimed to evaluate and compare the antimicrobial efficacy of CaOH and BlueM gel against F. nucleatum and E. faecalis biofilms using dentin discs. Sterilized dentin discs were prepared from extracted human teeth. F. nucleatum and E. faecalis were grown on these discs and then treated with either CaOH or BlueM gel. Antimicrobial efficacy was assessed by quantifying colony-forming unit counts. BlueM gel demonstrated significantly greater antimicrobial efficacy than CaOH against both F. nucleatum and E. faecalis biofilms. Both agents exhibited substantial antimicrobial activity against the biofilms. BlueM gel showed superior antimicrobial effectiveness against endodontic pathogens such as F. nucleatum and E. faecalis compared to CaOH.https://doi.org/10.1007/s44445-025-00042-xBlueMBiofilmCalcium hydroxideDentin discsE. faecalisF. nucleatum
spellingShingle Yahya Raja Alharbi
Swati Srivastava
Hanin Alsalhi
Khawlah Almohimeed
Mohammad Alduraibi
Ahmed Nabil
Antimicrobial efficacy of blueM gel and calcium hydroxide against F. nucleatum and E. faecalis biofilm: an ex-vivo study
Saudi Dental Journal
BlueM
Biofilm
Calcium hydroxide
Dentin discs
E. faecalis
F. nucleatum
title Antimicrobial efficacy of blueM gel and calcium hydroxide against F. nucleatum and E. faecalis biofilm: an ex-vivo study
title_full Antimicrobial efficacy of blueM gel and calcium hydroxide against F. nucleatum and E. faecalis biofilm: an ex-vivo study
title_fullStr Antimicrobial efficacy of blueM gel and calcium hydroxide against F. nucleatum and E. faecalis biofilm: an ex-vivo study
title_full_unstemmed Antimicrobial efficacy of blueM gel and calcium hydroxide against F. nucleatum and E. faecalis biofilm: an ex-vivo study
title_short Antimicrobial efficacy of blueM gel and calcium hydroxide against F. nucleatum and E. faecalis biofilm: an ex-vivo study
title_sort antimicrobial efficacy of bluem gel and calcium hydroxide against f nucleatum and e faecalis biofilm an ex vivo study
topic BlueM
Biofilm
Calcium hydroxide
Dentin discs
E. faecalis
F. nucleatum
url https://doi.org/10.1007/s44445-025-00042-x
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