Cloning and sequence analysis of PR-39 gene of porcine antibacterial peptide in bone marrow
Genome RNA was extracted from bone marrow of pig (Duroc × Landrace × Yorkshire) and PR-39 mRNA amplified through PT-PCR with special primers. A DNA fragment about 349 bp in length was obtained. The PCR product was cloned into pGEM-T vector and sequedced. Sequence analysis suggested that this fragmen...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2002-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2002.06.0685 |
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| _version_ | 1850044798938906624 |
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| author | WANG Yi-zheng YU Song-dong LIN Wen-xue |
| author_facet | WANG Yi-zheng YU Song-dong LIN Wen-xue |
| author_sort | WANG Yi-zheng |
| collection | DOAJ |
| description | Genome RNA was extracted from bone marrow of pig (Duroc × Landrace × Yorkshire) and PR-39 mRNA amplified through PT-PCR with special primers. A DNA fragment about 349 bp in length was obtained. The PCR product was cloned into pGEM-T vector and sequedced. Sequence analysis suggested that this fragment was partial sequence of pr-39 gene cDNA and coded 113 amino acid residues, which constituted the major part of pr-39 mature peptide. The gene fragment gained in this study was consistent with the porcine pr-39 cDNA partial sequence in Genebank. |
| format | Article |
| id | doaj-art-c33a6366b41a402f8959e09f8a5a4aad |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2002-11-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-c33a6366b41a402f8959e09f8a5a4aad2025-08-20T02:54:50ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552002-11-012868568810.3785/1008-9209.2002.06.068510089209Cloning and sequence analysis of PR-39 gene of porcine antibacterial peptide in bone marrowWANG Yi-zhengYU Song-dongLIN Wen-xueGenome RNA was extracted from bone marrow of pig (Duroc × Landrace × Yorkshire) and PR-39 mRNA amplified through PT-PCR with special primers. A DNA fragment about 349 bp in length was obtained. The PCR product was cloned into pGEM-T vector and sequedced. Sequence analysis suggested that this fragment was partial sequence of pr-39 gene cDNA and coded 113 amino acid residues, which constituted the major part of pr-39 mature peptide. The gene fragment gained in this study was consistent with the porcine pr-39 cDNA partial sequence in Genebank.https://www.academax.com/doi/10.3785/1008-9209.2002.06.0685pigbone marrowPR-39gene clonesequence analysis |
| spellingShingle | WANG Yi-zheng YU Song-dong LIN Wen-xue Cloning and sequence analysis of PR-39 gene of porcine antibacterial peptide in bone marrow 浙江大学学报. 农业与生命科学版 pig bone marrow PR-39 gene clone sequence analysis |
| title | Cloning and sequence analysis of PR-39 gene of porcine antibacterial peptide in bone marrow |
| title_full | Cloning and sequence analysis of PR-39 gene of porcine antibacterial peptide in bone marrow |
| title_fullStr | Cloning and sequence analysis of PR-39 gene of porcine antibacterial peptide in bone marrow |
| title_full_unstemmed | Cloning and sequence analysis of PR-39 gene of porcine antibacterial peptide in bone marrow |
| title_short | Cloning and sequence analysis of PR-39 gene of porcine antibacterial peptide in bone marrow |
| title_sort | cloning and sequence analysis of pr 39 gene of porcine antibacterial peptide in bone marrow |
| topic | pig bone marrow PR-39 gene clone sequence analysis |
| url | https://www.academax.com/doi/10.3785/1008-9209.2002.06.0685 |
| work_keys_str_mv | AT wangyizheng cloningandsequenceanalysisofpr39geneofporcineantibacterialpeptideinbonemarrow AT yusongdong cloningandsequenceanalysisofpr39geneofporcineantibacterialpeptideinbonemarrow AT linwenxue cloningandsequenceanalysisofpr39geneofporcineantibacterialpeptideinbonemarrow |