Toll-Like Receptor 2 Attenuates Traumatic Brain Injury-Induced Neural Stem Cell Proliferation in Dentate Gyrus of Rats
It was not clear how and whether neural stem cells (NSCs) responded to toll-like receptor 2 (TLR2) in the inflammatory environment after traumatic brain injury (TBI). The current study investigated the correlation of TLR2 and NSC proliferation in the dentate gyrus (DG) using the TBI model of rats. I...
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| Language: | English |
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Wiley
2020-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2020/9814978 |
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| author | Xin Zhang Yue Hei Wei Bai Tao Huang Enming Kang Huijun Chen Chuiguang Kong Yongxiang Yang Yuqin Ye Xiaosheng He |
| author_facet | Xin Zhang Yue Hei Wei Bai Tao Huang Enming Kang Huijun Chen Chuiguang Kong Yongxiang Yang Yuqin Ye Xiaosheng He |
| author_sort | Xin Zhang |
| collection | DOAJ |
| description | It was not clear how and whether neural stem cells (NSCs) responded to toll-like receptor 2 (TLR2) in the inflammatory environment after traumatic brain injury (TBI). The current study investigated the correlation of TLR2 and NSC proliferation in the dentate gyrus (DG) using the TBI model of rats. Immunofluorescence (IF) was used to observe the expression of BrdU, nestin, and TLR2 in the DG in morphology. Proliferating cells in the DG were labelled by thymidine analog 5-bromo-2-deoxyuridine (BrdU). Three-labelled BrdU, nestin, and DAPI was used for the identification of newly generated NSCs. Western blotting and real-time polymerase chain reaction (PCR) were used to observe the expression of TLR2 from the level of protein and mRNA. We observed that BrdU+/nestin+/DAPI+ cells accounted for 84.30%±6.54% among BrdU+ cells; BrdU+ and nestin+ cells in the DG were also TLR2+ cells. BrdU+ cells and the expression of TLR2 (both protein and mRNA levels) both elevated immediately at 6 hours (h), 24 h, 3 days (d), and 7 d posttrauma and peaked in 3 d. Results indicated that TLR2 was expressed on proliferating cells in the DG (NSCs possibly) and there was a potential correlation between increased TLR2 and proliferated NSCs after TBI. Taken together, these findings suggested that TLR2 was involved in endogenous neurogenesis in the DG after TBI. |
| format | Article |
| id | doaj-art-c339b239c5aa4da18cd89686bd9c43b2 |
| institution | DOAJ |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
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| series | Neural Plasticity |
| spelling | doaj-art-c339b239c5aa4da18cd89686bd9c43b22025-08-20T03:23:15ZengWileyNeural Plasticity2090-59041687-54432020-01-01202010.1155/2020/98149789814978Toll-Like Receptor 2 Attenuates Traumatic Brain Injury-Induced Neural Stem Cell Proliferation in Dentate Gyrus of RatsXin Zhang0Yue Hei1Wei Bai2Tao Huang3Enming Kang4Huijun Chen5Chuiguang Kong6Yongxiang Yang7Yuqin Ye8Xiaosheng He9Department of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi’an, 710032 Shaanxi, ChinaDepartment of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi’an, 710032 Shaanxi, ChinaDepartment of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi’an, 710032 Shaanxi, ChinaDepartment of Neurosurgery, Tangdu Hospital, Air Force Medical University, Xi’an, 710032 Shaanxi, ChinaDepartment of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi’an, 710032 Shaanxi, ChinaDepartment of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi’an, 710032 Shaanxi, ChinaDepartment of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi’an, 710032 Shaanxi, ChinaDepartment of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi’an, 710032 Shaanxi, ChinaDepartment of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi’an, 710032 Shaanxi, ChinaDepartment of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi’an, 710032 Shaanxi, ChinaIt was not clear how and whether neural stem cells (NSCs) responded to toll-like receptor 2 (TLR2) in the inflammatory environment after traumatic brain injury (TBI). The current study investigated the correlation of TLR2 and NSC proliferation in the dentate gyrus (DG) using the TBI model of rats. Immunofluorescence (IF) was used to observe the expression of BrdU, nestin, and TLR2 in the DG in morphology. Proliferating cells in the DG were labelled by thymidine analog 5-bromo-2-deoxyuridine (BrdU). Three-labelled BrdU, nestin, and DAPI was used for the identification of newly generated NSCs. Western blotting and real-time polymerase chain reaction (PCR) were used to observe the expression of TLR2 from the level of protein and mRNA. We observed that BrdU+/nestin+/DAPI+ cells accounted for 84.30%±6.54% among BrdU+ cells; BrdU+ and nestin+ cells in the DG were also TLR2+ cells. BrdU+ cells and the expression of TLR2 (both protein and mRNA levels) both elevated immediately at 6 hours (h), 24 h, 3 days (d), and 7 d posttrauma and peaked in 3 d. Results indicated that TLR2 was expressed on proliferating cells in the DG (NSCs possibly) and there was a potential correlation between increased TLR2 and proliferated NSCs after TBI. Taken together, these findings suggested that TLR2 was involved in endogenous neurogenesis in the DG after TBI.http://dx.doi.org/10.1155/2020/9814978 |
| spellingShingle | Xin Zhang Yue Hei Wei Bai Tao Huang Enming Kang Huijun Chen Chuiguang Kong Yongxiang Yang Yuqin Ye Xiaosheng He Toll-Like Receptor 2 Attenuates Traumatic Brain Injury-Induced Neural Stem Cell Proliferation in Dentate Gyrus of Rats Neural Plasticity |
| title | Toll-Like Receptor 2 Attenuates Traumatic Brain Injury-Induced Neural Stem Cell Proliferation in Dentate Gyrus of Rats |
| title_full | Toll-Like Receptor 2 Attenuates Traumatic Brain Injury-Induced Neural Stem Cell Proliferation in Dentate Gyrus of Rats |
| title_fullStr | Toll-Like Receptor 2 Attenuates Traumatic Brain Injury-Induced Neural Stem Cell Proliferation in Dentate Gyrus of Rats |
| title_full_unstemmed | Toll-Like Receptor 2 Attenuates Traumatic Brain Injury-Induced Neural Stem Cell Proliferation in Dentate Gyrus of Rats |
| title_short | Toll-Like Receptor 2 Attenuates Traumatic Brain Injury-Induced Neural Stem Cell Proliferation in Dentate Gyrus of Rats |
| title_sort | toll like receptor 2 attenuates traumatic brain injury induced neural stem cell proliferation in dentate gyrus of rats |
| url | http://dx.doi.org/10.1155/2020/9814978 |
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