Interferonβ-1b Induces the Expression of RGS1 a Negative Regulator of G-Protein Signaling
We present evidence of a link between interferonβ-1b (IFN-β) and G-protein signaling by demonstrating that IFN-β can induce the expression of the negative regulator of G-protein signaling 1 (RGS1). RGS1 reduces G-protein activation and immune cell migration by interacting with heterotrimeric G-prote...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | International Journal of Cell Biology |
| Online Access: | http://dx.doi.org/10.1155/2010/529376 |
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| _version_ | 1849685150807359488 |
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| author | Tiffany Tran Pedro Paz Sharlene Velichko Jill Cifrese Praveen Belur Ken D. Yamaguchi Karin Ku Parham Mirshahpanah Anthony T. Reder Ed Croze |
| author_facet | Tiffany Tran Pedro Paz Sharlene Velichko Jill Cifrese Praveen Belur Ken D. Yamaguchi Karin Ku Parham Mirshahpanah Anthony T. Reder Ed Croze |
| author_sort | Tiffany Tran |
| collection | DOAJ |
| description | We present evidence of a link between interferonβ-1b (IFN-β) and G-protein signaling by demonstrating that IFN-β can induce the expression of the negative regulator of G-protein signaling 1 (RGS1). RGS1 reduces G-protein activation and immune cell migration by interacting with heterotrimeric G-proteins and enhancing their intrinsic GTPase activity. In this study, IFN-β treatment resulted in the induction of RGS1 in peripheral blood mononuclear cells (PBMCs), monocytes, T cells, and B cells. Induction of RGS1 by IFN-β was concentration dependent and observed at both the RNA and protein level. Other members of the RGS family were not induced by IFN-β, and induction of RGS1 required the activation of the IFN receptor. In addition, RGS1 induction was observed in PBMCs obtained from IFN-β-treated multiple sclerosis patients suggesting a possible, as yet unexplored, involvement of G-protein regulation in disease treatment. The upregulation of RGS1 by IFN-β has not been previously reported. |
| format | Article |
| id | doaj-art-c334a8a87298419992e46ce8c0e9b067 |
| institution | DOAJ |
| issn | 1687-8876 1687-8884 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Cell Biology |
| spelling | doaj-art-c334a8a87298419992e46ce8c0e9b0672025-08-20T03:23:15ZengWileyInternational Journal of Cell Biology1687-88761687-88842010-01-01201010.1155/2010/529376529376Interferonβ-1b Induces the Expression of RGS1 a Negative Regulator of G-Protein SignalingTiffany Tran0Pedro Paz1Sharlene Velichko2Jill Cifrese3Praveen Belur4Ken D. Yamaguchi5Karin Ku6Parham Mirshahpanah7Anthony T. Reder8Ed Croze9Bayer HealthCare, Applied Research, Richmond, CA 94804, USABayer HealthCare, Applied Research, Richmond, CA 94804, USABayer HealthCare, Applied Research, Richmond, CA 94804, USABayer HealthCare, Applied Research, Richmond, CA 94804, USABayer HealthCare, Applied Research, Richmond, CA 94804, USABayer HealthCare, Applied Research, Richmond, CA 94804, USABayer HealthCare, Applied Research, Richmond, CA 94804, USABayer HealthCare, Applied Research, Richmond, CA 94804, USADepartment of Neurology, University of Chicago, Chicago, IL 60687, USABayer HealthCare, Applied Research, Richmond, CA 94804, USAWe present evidence of a link between interferonβ-1b (IFN-β) and G-protein signaling by demonstrating that IFN-β can induce the expression of the negative regulator of G-protein signaling 1 (RGS1). RGS1 reduces G-protein activation and immune cell migration by interacting with heterotrimeric G-proteins and enhancing their intrinsic GTPase activity. In this study, IFN-β treatment resulted in the induction of RGS1 in peripheral blood mononuclear cells (PBMCs), monocytes, T cells, and B cells. Induction of RGS1 by IFN-β was concentration dependent and observed at both the RNA and protein level. Other members of the RGS family were not induced by IFN-β, and induction of RGS1 required the activation of the IFN receptor. In addition, RGS1 induction was observed in PBMCs obtained from IFN-β-treated multiple sclerosis patients suggesting a possible, as yet unexplored, involvement of G-protein regulation in disease treatment. The upregulation of RGS1 by IFN-β has not been previously reported.http://dx.doi.org/10.1155/2010/529376 |
| spellingShingle | Tiffany Tran Pedro Paz Sharlene Velichko Jill Cifrese Praveen Belur Ken D. Yamaguchi Karin Ku Parham Mirshahpanah Anthony T. Reder Ed Croze Interferonβ-1b Induces the Expression of RGS1 a Negative Regulator of G-Protein Signaling International Journal of Cell Biology |
| title | Interferonβ-1b Induces the Expression of RGS1 a Negative Regulator of G-Protein Signaling |
| title_full | Interferonβ-1b Induces the Expression of RGS1 a Negative Regulator of G-Protein Signaling |
| title_fullStr | Interferonβ-1b Induces the Expression of RGS1 a Negative Regulator of G-Protein Signaling |
| title_full_unstemmed | Interferonβ-1b Induces the Expression of RGS1 a Negative Regulator of G-Protein Signaling |
| title_short | Interferonβ-1b Induces the Expression of RGS1 a Negative Regulator of G-Protein Signaling |
| title_sort | interferonβ 1b induces the expression of rgs1 a negative regulator of g protein signaling |
| url | http://dx.doi.org/10.1155/2010/529376 |
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