Impact of hyaluronic acid with different molecular weights on the functional properties of processed cheese products with varying moisture levels

Hyaluronic acid (HA) binds moisture, and as the molecular weight (MW) of HA increases, the moisture binding capacity increases, which affects the functionality of the products. This study investigated the effect of HA with different MWs on the functionality of process cheese products (PCPs) at varyi...

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Main Authors: Rutvi Joshi, Suresh G. Sutariya, Prafulla Salunke
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Applied Food Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772502225003026
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author Rutvi Joshi
Suresh G. Sutariya
Prafulla Salunke
author_facet Rutvi Joshi
Suresh G. Sutariya
Prafulla Salunke
author_sort Rutvi Joshi
collection DOAJ
description Hyaluronic acid (HA) binds moisture, and as the molecular weight (MW) of HA increases, the moisture binding capacity increases, which affects the functionality of the products. This study investigated the effect of HA with different MWs on the functionality of process cheese products (PCPs) at varying moisture levels. The aim was to assess HA as a natural alternative to chemical additives and determine its potential to enhance moisture retention due to its high water-binding capacity. Treatment PCPs with 0.15 % HA (8 kDa, 320 kDa, 980 kDa, and 2550 kDa MW) were formulated at target moisture levels of 51 %, 54 %, 57 %, and 60 %, replacing emulsifying salt (ES). The control sample contained ES but no HA. PCPs were analyzed for the unmelted and melted properties. Results showed that substituting ES with HA enabled PCP manufacturing at various moisture levels. Moisture and HA significantly impacted PCPs’ unmelted and melted properties compared to the control. As the moisture increased, there was a decrease in hardness and an increase in melting characteristics. As the MW of HA increased, the water binding capacity increased, leading to PCP formation. HA addition in PCP significantly restricted the melting properties and increased hardness, which depended on the MW of HA. PCP manufacturing at 60 % moisture, using ES, was not possible, but with HA, it was possible to manufacture PCP with a firmer product and restricted melt. Hence, HA can replace ES where PCP with higher moisture is required, along with the functional benefits of the firmer product and restricted melt.
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spelling doaj-art-c321ddefb27641578020f728325a810d2025-08-20T03:20:12ZengElsevierApplied Food Research2772-50222025-06-015110099410.1016/j.afres.2025.100994Impact of hyaluronic acid with different molecular weights on the functional properties of processed cheese products with varying moisture levelsRutvi Joshi0Suresh G. Sutariya1Prafulla Salunke2Dairy and Food Science Department, South Dakota State University, Brookings, SD 57007, USADairy and Food Science Department, South Dakota State University, Brookings, SD 57007, USACorresponding author.; Dairy and Food Science Department, South Dakota State University, Brookings, SD 57007, USAHyaluronic acid (HA) binds moisture, and as the molecular weight (MW) of HA increases, the moisture binding capacity increases, which affects the functionality of the products. This study investigated the effect of HA with different MWs on the functionality of process cheese products (PCPs) at varying moisture levels. The aim was to assess HA as a natural alternative to chemical additives and determine its potential to enhance moisture retention due to its high water-binding capacity. Treatment PCPs with 0.15 % HA (8 kDa, 320 kDa, 980 kDa, and 2550 kDa MW) were formulated at target moisture levels of 51 %, 54 %, 57 %, and 60 %, replacing emulsifying salt (ES). The control sample contained ES but no HA. PCPs were analyzed for the unmelted and melted properties. Results showed that substituting ES with HA enabled PCP manufacturing at various moisture levels. Moisture and HA significantly impacted PCPs’ unmelted and melted properties compared to the control. As the moisture increased, there was a decrease in hardness and an increase in melting characteristics. As the MW of HA increased, the water binding capacity increased, leading to PCP formation. HA addition in PCP significantly restricted the melting properties and increased hardness, which depended on the MW of HA. PCP manufacturing at 60 % moisture, using ES, was not possible, but with HA, it was possible to manufacture PCP with a firmer product and restricted melt. Hence, HA can replace ES where PCP with higher moisture is required, along with the functional benefits of the firmer product and restricted melt.http://www.sciencedirect.com/science/article/pii/S2772502225003026Hyaluronic acidFunctional propertiesProcess cheese productsMelting propertiesNon-melting properties
spellingShingle Rutvi Joshi
Suresh G. Sutariya
Prafulla Salunke
Impact of hyaluronic acid with different molecular weights on the functional properties of processed cheese products with varying moisture levels
Applied Food Research
Hyaluronic acid
Functional properties
Process cheese products
Melting properties
Non-melting properties
title Impact of hyaluronic acid with different molecular weights on the functional properties of processed cheese products with varying moisture levels
title_full Impact of hyaluronic acid with different molecular weights on the functional properties of processed cheese products with varying moisture levels
title_fullStr Impact of hyaluronic acid with different molecular weights on the functional properties of processed cheese products with varying moisture levels
title_full_unstemmed Impact of hyaluronic acid with different molecular weights on the functional properties of processed cheese products with varying moisture levels
title_short Impact of hyaluronic acid with different molecular weights on the functional properties of processed cheese products with varying moisture levels
title_sort impact of hyaluronic acid with different molecular weights on the functional properties of processed cheese products with varying moisture levels
topic Hyaluronic acid
Functional properties
Process cheese products
Melting properties
Non-melting properties
url http://www.sciencedirect.com/science/article/pii/S2772502225003026
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AT prafullasalunke impactofhyaluronicacidwithdifferentmolecularweightsonthefunctionalpropertiesofprocessedcheeseproductswithvaryingmoisturelevels