Preparation and in vitro evaluation of platelet membrane biomimetic liposomes loaded with vincristine sulfate
[Objective] To prepare platelet membrane biomimetic liposomes loaded with vincristine sulfate (VCR) for targeted delivery to tumor. [Methods] Vincristine sulfate liposomes (LIPO) were prepared using the pH-gradient method, followed by the fusion of platelet membranes and subsequent drug loading to o...
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Institute of Blood Transfusion of Chinese Academy of Medical Sciences
2025-05-01
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| Series: | Zhongguo shuxue zazhi |
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| Online Access: | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.05.009&lang=en |
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| author | XIAO Jing YOU Xunyi ZHANG Along ZHONG Rui LIU Jiaxin CAO Ye WANG Hong |
| author_facet | XIAO Jing YOU Xunyi ZHANG Along ZHONG Rui LIU Jiaxin CAO Ye WANG Hong |
| author_sort | XIAO Jing |
| collection | DOAJ |
| description | [Objective] To prepare platelet membrane biomimetic liposomes loaded with vincristine sulfate (VCR) for targeted delivery to tumor. [Methods] Vincristine sulfate liposomes (LIPO) were prepared using the pH-gradient method, followed by the fusion of platelet membranes and subsequent drug loading to obtain platelet membrane biomimetic liposomes (PLM-LIPO). The particle size, polydispersity index (PDI), Zeta potential, and drug encapsulation efficiency (EE%) of both liposomes were characterized. The tumor-targeting capability was evaluated through in vitro cellular experiments and in vivo biodistribution studies. [Results] The optimal preparation conditions for LIPO were determined as follows: DPPC-to-cholesterol molar ratio of 1∶1, internal aqueous phase of 0.3 M pH 4.0 citrate buffer, external aqueous phase of 1 M Na2HPO4 solution, drug-to-lipid ratio of 1∶10, drug loading temperature of 60℃, and loading time of 10 minutes. The LIPO exhibited a mean particle size of (147.3±2.24) nm, PDI of 0.078±0.014, Zeta potential of (-3.54±0.75) mV, and EE% of 91.37±0.47. For PLM-LIPO, prepared via membrane fusion followed by drug loading, the mean particle size was (185.3±3.61) nm, PDI was 0.075±0.022, Zeta potential was (-18.91±1.54) mV, and EE% was 63.36±2.45. In the CD62P validation experiment, the fluorescence intensity of PLM-LIPO was five times higher than that of LIPO. In vitro cellular uptake experiments revealed that PLM-LIPO showed 1.3-fold and 1.2-fold higher uptake rates compared to LIPO at 6 h and 12 h, respectively. In vivo experiments demonstrated that 1h after administration, the accumulation of PLM-LIPO at tumor sites was 4-fold higher than that of LIPO and 6-7 times higher than that in healthy mice. [Conclusion] The platelet membrane biomimetic liposomes loaded with vincristine sulfate were successfully developed. Both cellular uptake and tissue distribution studies confirmed the PLM-LIPO enhanced tumor-targeting capability. |
| format | Article |
| id | doaj-art-c31bcdf82c1c40cb82456d59690ca866 |
| institution | OA Journals |
| issn | 1004-549X |
| language | zho |
| publishDate | 2025-05-01 |
| publisher | Institute of Blood Transfusion of Chinese Academy of Medical Sciences |
| record_format | Article |
| series | Zhongguo shuxue zazhi |
| spelling | doaj-art-c31bcdf82c1c40cb82456d59690ca8662025-08-20T02:29:19ZzhoInstitute of Blood Transfusion of Chinese Academy of Medical SciencesZhongguo shuxue zazhi1004-549X2025-05-0138565265910.13303/j.cjbt.issn.1004-549x.2025.05.0091004-549X(2025)5-0652-08Preparation and in vitro evaluation of platelet membrane biomimetic liposomes loaded with vincristine sulfateXIAO Jing0YOU Xunyi1ZHANG Along2ZHONG Rui3LIU Jiaxin4CAO Ye5WANG Hong6Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, China[Objective] To prepare platelet membrane biomimetic liposomes loaded with vincristine sulfate (VCR) for targeted delivery to tumor. [Methods] Vincristine sulfate liposomes (LIPO) were prepared using the pH-gradient method, followed by the fusion of platelet membranes and subsequent drug loading to obtain platelet membrane biomimetic liposomes (PLM-LIPO). The particle size, polydispersity index (PDI), Zeta potential, and drug encapsulation efficiency (EE%) of both liposomes were characterized. The tumor-targeting capability was evaluated through in vitro cellular experiments and in vivo biodistribution studies. [Results] The optimal preparation conditions for LIPO were determined as follows: DPPC-to-cholesterol molar ratio of 1∶1, internal aqueous phase of 0.3 M pH 4.0 citrate buffer, external aqueous phase of 1 M Na2HPO4 solution, drug-to-lipid ratio of 1∶10, drug loading temperature of 60℃, and loading time of 10 minutes. The LIPO exhibited a mean particle size of (147.3±2.24) nm, PDI of 0.078±0.014, Zeta potential of (-3.54±0.75) mV, and EE% of 91.37±0.47. For PLM-LIPO, prepared via membrane fusion followed by drug loading, the mean particle size was (185.3±3.61) nm, PDI was 0.075±0.022, Zeta potential was (-18.91±1.54) mV, and EE% was 63.36±2.45. In the CD62P validation experiment, the fluorescence intensity of PLM-LIPO was five times higher than that of LIPO. In vitro cellular uptake experiments revealed that PLM-LIPO showed 1.3-fold and 1.2-fold higher uptake rates compared to LIPO at 6 h and 12 h, respectively. In vivo experiments demonstrated that 1h after administration, the accumulation of PLM-LIPO at tumor sites was 4-fold higher than that of LIPO and 6-7 times higher than that in healthy mice. [Conclusion] The platelet membrane biomimetic liposomes loaded with vincristine sulfate were successfully developed. Both cellular uptake and tissue distribution studies confirmed the PLM-LIPO enhanced tumor-targeting capability.https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.05.009&lang=enplatelet membraneliposomesvincristine sulfatetumor targeted therapy |
| spellingShingle | XIAO Jing YOU Xunyi ZHANG Along ZHONG Rui LIU Jiaxin CAO Ye WANG Hong Preparation and in vitro evaluation of platelet membrane biomimetic liposomes loaded with vincristine sulfate Zhongguo shuxue zazhi platelet membrane liposomes vincristine sulfate tumor targeted therapy |
| title | Preparation and in vitro evaluation of platelet membrane biomimetic liposomes loaded with vincristine sulfate |
| title_full | Preparation and in vitro evaluation of platelet membrane biomimetic liposomes loaded with vincristine sulfate |
| title_fullStr | Preparation and in vitro evaluation of platelet membrane biomimetic liposomes loaded with vincristine sulfate |
| title_full_unstemmed | Preparation and in vitro evaluation of platelet membrane biomimetic liposomes loaded with vincristine sulfate |
| title_short | Preparation and in vitro evaluation of platelet membrane biomimetic liposomes loaded with vincristine sulfate |
| title_sort | preparation and in vitro evaluation of platelet membrane biomimetic liposomes loaded with vincristine sulfate |
| topic | platelet membrane liposomes vincristine sulfate tumor targeted therapy |
| url | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.05.009&lang=en |
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