Elucidating time divergence and biogeography of Euonymus hamiltonianus complex using complete plastome analysis

Abstract The evolutionary history of Euonymus L., within the Celastraceae family has been understudied. Within this genus E. hamiltonianus Wall., belonging to E. sect. Biloculares have slight variations in morphology due to their high ecological range, leading to different varieties and subspecies....

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Main Authors: Shayan Jamshed, Joo-Hwan Kim
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-13321-8
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author Shayan Jamshed
Joo-Hwan Kim
author_facet Shayan Jamshed
Joo-Hwan Kim
author_sort Shayan Jamshed
collection DOAJ
description Abstract The evolutionary history of Euonymus L., within the Celastraceae family has been understudied. Within this genus E. hamiltonianus Wall., belonging to E. sect. Biloculares have slight variations in morphology due to their high ecological range, leading to different varieties and subspecies. This has caused debate on the species status of many taxa. This study we investigate the plastome of E. hamiltonianus complex and related species in E. sect. Biloculares. It was aimed to observe the similarities and differences in the plastome, to clarify the ambiguities and evolutionary relationships within this complex. Plastome showed high similarities in coding regions and conserved quadripartite structure (156,881–158,434 bp). E. hamiltonianus complex had rps19 located in the LSC region. ndhF was reportedly a pseudogene in E. hamiltonianus (Mugabsan, South Korea). In all the investigated species, rps16 lacked introns. A resolved relation was observed among the taxa of E. hamiltonianus complex, and the species closely related to these (100% Bootstrap & 1.0 PP). E. sieboldianus Blume was observed at a position between the two taxa of E. hamiltonianus, making it a possible synonymy. E. europaeus L., and E. velutinus Fisch. & C.A.Mey., formed separate lineages. Molecular dating suggests that E. hamiltonianus complex originated in East Asia during the Middle Miocene Transition, which is a significant phase within the Neogene era, supporting the hypothesis that Euonymus originated in East Asia and E. hamiltonianus complex is estimated to develop in East Asia and diversified across the Far East during this period.
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spelling doaj-art-c312d7cae1c144c0b2b98a2314dae8ac2025-08-20T03:45:49ZengNature PortfolioScientific Reports2045-23222025-08-0115111510.1038/s41598-025-13321-8Elucidating time divergence and biogeography of Euonymus hamiltonianus complex using complete plastome analysisShayan Jamshed0Joo-Hwan Kim1Department of Life Sciences, Gachon UniversityDepartment of Life Sciences, Gachon UniversityAbstract The evolutionary history of Euonymus L., within the Celastraceae family has been understudied. Within this genus E. hamiltonianus Wall., belonging to E. sect. Biloculares have slight variations in morphology due to their high ecological range, leading to different varieties and subspecies. This has caused debate on the species status of many taxa. This study we investigate the plastome of E. hamiltonianus complex and related species in E. sect. Biloculares. It was aimed to observe the similarities and differences in the plastome, to clarify the ambiguities and evolutionary relationships within this complex. Plastome showed high similarities in coding regions and conserved quadripartite structure (156,881–158,434 bp). E. hamiltonianus complex had rps19 located in the LSC region. ndhF was reportedly a pseudogene in E. hamiltonianus (Mugabsan, South Korea). In all the investigated species, rps16 lacked introns. A resolved relation was observed among the taxa of E. hamiltonianus complex, and the species closely related to these (100% Bootstrap & 1.0 PP). E. sieboldianus Blume was observed at a position between the two taxa of E. hamiltonianus, making it a possible synonymy. E. europaeus L., and E. velutinus Fisch. & C.A.Mey., formed separate lineages. Molecular dating suggests that E. hamiltonianus complex originated in East Asia during the Middle Miocene Transition, which is a significant phase within the Neogene era, supporting the hypothesis that Euonymus originated in East Asia and E. hamiltonianus complex is estimated to develop in East Asia and diversified across the Far East during this period.https://doi.org/10.1038/s41598-025-13321-8DisjunctionEuonymus hamiltonianus complexEuonymus sect. BilocularesEast AsiaWest AsiaNorth America
spellingShingle Shayan Jamshed
Joo-Hwan Kim
Elucidating time divergence and biogeography of Euonymus hamiltonianus complex using complete plastome analysis
Scientific Reports
Disjunction
Euonymus hamiltonianus complex
Euonymus sect. Biloculares
East Asia
West Asia
North America
title Elucidating time divergence and biogeography of Euonymus hamiltonianus complex using complete plastome analysis
title_full Elucidating time divergence and biogeography of Euonymus hamiltonianus complex using complete plastome analysis
title_fullStr Elucidating time divergence and biogeography of Euonymus hamiltonianus complex using complete plastome analysis
title_full_unstemmed Elucidating time divergence and biogeography of Euonymus hamiltonianus complex using complete plastome analysis
title_short Elucidating time divergence and biogeography of Euonymus hamiltonianus complex using complete plastome analysis
title_sort elucidating time divergence and biogeography of euonymus hamiltonianus complex using complete plastome analysis
topic Disjunction
Euonymus hamiltonianus complex
Euonymus sect. Biloculares
East Asia
West Asia
North America
url https://doi.org/10.1038/s41598-025-13321-8
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AT joohwankim elucidatingtimedivergenceandbiogeographyofeuonymushamiltonianuscomplexusingcompleteplastomeanalysis