CitGATA7 interact with histone acetyltransferase CitHAG28 to promote citric acid degradation by regulating the glutamine synthetase pathway in citrus
Abstract Organic acid is a crucial indicator of fruit quality traits. Citric acid, the predominant organic acid in citrus fruit, directly influences its edible quality and economic value. While the transcriptional regulatory mechanisms of citric acid metabolism have been extensively studied, the und...
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| Language: | English |
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BMC
2025-02-01
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| Series: | Molecular Horticulture |
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| Online Access: | https://doi.org/10.1186/s43897-024-00126-y |
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| author | Xiahui Lin Shaojia Li Yanna Shi Yuchen Ma Yinchun Li Haohan Tan Bo Zhang Changjie Xu Kunsong Chen |
| author_facet | Xiahui Lin Shaojia Li Yanna Shi Yuchen Ma Yinchun Li Haohan Tan Bo Zhang Changjie Xu Kunsong Chen |
| author_sort | Xiahui Lin |
| collection | DOAJ |
| description | Abstract Organic acid is a crucial indicator of fruit quality traits. Citric acid, the predominant organic acid in citrus fruit, directly influences its edible quality and economic value. While the transcriptional regulatory mechanisms of citric acid metabolism have been extensively studied, the understanding about the transcriptional and epigenetic co-regulation mechanisms is limited. This study characterized a transcription factor, CitGATA7, which directly binds to and activates the expression of genes associated with the glutamine synthetase pathway regulating citric acid degradation. These genes include the aconitase encoding gene CitACO3, the isocitrate dehydrogenase encoding gene CitIDH1, and the glutamine synthetase encoding gene CitGS1. Furthermore, CitGATA7 physically interacts with the histone acetyltransferase CitHAG28 to enhance histone 3 acetylation levels near the transcription start site of CitACO3, CitIDH1, and CitGS1, thereby increasing their transcription and promoting citric acid degradation. The findings demonstrate that the CitGATA7-CitHAG28 protein complex transcriptionally regulate the expression of the GS pathway genes, i.e., CitACO3, CitIDH1, and CitGS1, via histone acetylation, thus promoting citric acid catabolism. This study establishes a direct link between transcriptional regulation and histone acetylation regarding citric acid metabolism, providing insights for strategies to manipulate organic acid accumulation in fruit. |
| format | Article |
| id | doaj-art-c31042500cd5424fa1545f34c8852b44 |
| institution | Kabale University |
| issn | 2730-9401 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Horticulture |
| spelling | doaj-art-c31042500cd5424fa1545f34c8852b442025-02-02T12:42:34ZengBMCMolecular Horticulture2730-94012025-02-015111410.1186/s43897-024-00126-yCitGATA7 interact with histone acetyltransferase CitHAG28 to promote citric acid degradation by regulating the glutamine synthetase pathway in citrusXiahui Lin0Shaojia Li1Yanna Shi2Yuchen Ma3Yinchun Li4Haohan Tan5Bo Zhang6Changjie Xu7Kunsong Chen8College of Agriculture and Biotechnology, Zhejiang UniversityCollege of Agriculture and Biotechnology, Zhejiang UniversityCollege of Agriculture and Biotechnology, Zhejiang UniversityCollege of Agriculture and Biotechnology, Zhejiang UniversityCollege of Agriculture and Biotechnology, Zhejiang UniversityCollege of Agriculture and Biotechnology, Zhejiang UniversityCollege of Agriculture and Biotechnology, Zhejiang UniversityCollege of Agriculture and Biotechnology, Zhejiang UniversityCollege of Agriculture and Biotechnology, Zhejiang UniversityAbstract Organic acid is a crucial indicator of fruit quality traits. Citric acid, the predominant organic acid in citrus fruit, directly influences its edible quality and economic value. While the transcriptional regulatory mechanisms of citric acid metabolism have been extensively studied, the understanding about the transcriptional and epigenetic co-regulation mechanisms is limited. This study characterized a transcription factor, CitGATA7, which directly binds to and activates the expression of genes associated with the glutamine synthetase pathway regulating citric acid degradation. These genes include the aconitase encoding gene CitACO3, the isocitrate dehydrogenase encoding gene CitIDH1, and the glutamine synthetase encoding gene CitGS1. Furthermore, CitGATA7 physically interacts with the histone acetyltransferase CitHAG28 to enhance histone 3 acetylation levels near the transcription start site of CitACO3, CitIDH1, and CitGS1, thereby increasing their transcription and promoting citric acid degradation. The findings demonstrate that the CitGATA7-CitHAG28 protein complex transcriptionally regulate the expression of the GS pathway genes, i.e., CitACO3, CitIDH1, and CitGS1, via histone acetylation, thus promoting citric acid catabolism. This study establishes a direct link between transcriptional regulation and histone acetylation regarding citric acid metabolism, providing insights for strategies to manipulate organic acid accumulation in fruit.https://doi.org/10.1186/s43897-024-00126-yCitrusCitric acidTranscriptional regulationHistone acetylationHabitats |
| spellingShingle | Xiahui Lin Shaojia Li Yanna Shi Yuchen Ma Yinchun Li Haohan Tan Bo Zhang Changjie Xu Kunsong Chen CitGATA7 interact with histone acetyltransferase CitHAG28 to promote citric acid degradation by regulating the glutamine synthetase pathway in citrus Molecular Horticulture Citrus Citric acid Transcriptional regulation Histone acetylation Habitats |
| title | CitGATA7 interact with histone acetyltransferase CitHAG28 to promote citric acid degradation by regulating the glutamine synthetase pathway in citrus |
| title_full | CitGATA7 interact with histone acetyltransferase CitHAG28 to promote citric acid degradation by regulating the glutamine synthetase pathway in citrus |
| title_fullStr | CitGATA7 interact with histone acetyltransferase CitHAG28 to promote citric acid degradation by regulating the glutamine synthetase pathway in citrus |
| title_full_unstemmed | CitGATA7 interact with histone acetyltransferase CitHAG28 to promote citric acid degradation by regulating the glutamine synthetase pathway in citrus |
| title_short | CitGATA7 interact with histone acetyltransferase CitHAG28 to promote citric acid degradation by regulating the glutamine synthetase pathway in citrus |
| title_sort | citgata7 interact with histone acetyltransferase cithag28 to promote citric acid degradation by regulating the glutamine synthetase pathway in citrus |
| topic | Citrus Citric acid Transcriptional regulation Histone acetylation Habitats |
| url | https://doi.org/10.1186/s43897-024-00126-y |
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