Syphacia obvelata antigens alter the FOXP3/RORɣt expression balance in isolated peripheral blood mononuclear cells of IBD patients
Abstract Inflammatory Bowel Disease (IBD) arises from disrupted interactions among intestinal microbiota, epithelial cells, and the immune system, which are influenced by genetic and environmental factors. A critical factor in IBD pathogenesis is the balance between FOXP3+ regulatory T cells (Tregs)...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-06619-0 |
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| author | Abbas Amin Niloofar Taghipour Mohammad Rostami-Nejad Foroogh Alborzi Avanaki Reyhaneh Jafarshad Seyyed Javad Seyyed Tabaei Nariman Mosaffa |
| author_facet | Abbas Amin Niloofar Taghipour Mohammad Rostami-Nejad Foroogh Alborzi Avanaki Reyhaneh Jafarshad Seyyed Javad Seyyed Tabaei Nariman Mosaffa |
| author_sort | Abbas Amin |
| collection | DOAJ |
| description | Abstract Inflammatory Bowel Disease (IBD) arises from disrupted interactions among intestinal microbiota, epithelial cells, and the immune system, which are influenced by genetic and environmental factors. A critical factor in IBD pathogenesis is the balance between FOXP3+ regulatory T cells (Tregs) and RORγt+ Th17 cells; a decreased Treg/Th17 ratio can lead to inflammation. Syphacia obvelata may help modulate immune responses by promoting Th2 responses and enhancing Treg populations, potentially impacting FOXP3 and RORγt expression and aiding IBD management. In this study, peripheral Blood Mononuclear Cells (PBMCs) from 6 IBD patients were treated with S. obvelata antigens (ES-Ag, S-Ag, and ES/S-Ag) for 24 h. Optimal concentrations and time points were determined via the MTT assay. Total RNA was extracted, cDNA was synthesized, and RT-qPCR was performed using FOXP3 and RORγt primers. The gene expression fold changes and FOXP3/RORγt ratios were compared to the control group. Results showed that FOXP3 expression was increased significantly after treatment with all S. obvelata antigens, including ES-Ag, S-Ag, and ES/S-Ag, whereas the expression of RORγt decreased significantly in just two groups, including ES-Ag and ES/S-Ag. Eventually, the FOXP3/RORγt gene expression fold change ratio was increased significantly after 24 h of exposure to S. obvelata antigens. Our study indicates that the ES-Ag, S-Ag, and ES/S-Ag of S. obvelata, along with their combination, can increase the FOXP3/RORɣt ratio in PBMCs from patients with IBD, suggesting anti-inflammatory effects. This finding offers hope that these antigens could be an eye-opener for developing future strategies for IBD treatment. |
| format | Article |
| id | doaj-art-c30a5d3aeb984e6da1e50e57cae2cc07 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-c30a5d3aeb984e6da1e50e57cae2cc072025-08-20T03:04:24ZengNature PortfolioScientific Reports2045-23222025-07-011511910.1038/s41598-025-06619-0Syphacia obvelata antigens alter the FOXP3/RORɣt expression balance in isolated peripheral blood mononuclear cells of IBD patientsAbbas Amin0Niloofar Taghipour1Mohammad Rostami-Nejad2Foroogh Alborzi Avanaki3Reyhaneh Jafarshad4Seyyed Javad Seyyed Tabaei5Nariman Mosaffa6Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical SciencesDepartment of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical SciencesCeliac Disease and Gluten Related Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical SciencesImam Khomeini Hospital GI Ward, Tehran University of Medical SciencesImam Khomeini Hospital GI Ward, Tehran University of Medical SciencesDepartment of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical SciencesDepartment of Immunology, School of Medicine, Shahid Beheshti University of Medical SciencesAbstract Inflammatory Bowel Disease (IBD) arises from disrupted interactions among intestinal microbiota, epithelial cells, and the immune system, which are influenced by genetic and environmental factors. A critical factor in IBD pathogenesis is the balance between FOXP3+ regulatory T cells (Tregs) and RORγt+ Th17 cells; a decreased Treg/Th17 ratio can lead to inflammation. Syphacia obvelata may help modulate immune responses by promoting Th2 responses and enhancing Treg populations, potentially impacting FOXP3 and RORγt expression and aiding IBD management. In this study, peripheral Blood Mononuclear Cells (PBMCs) from 6 IBD patients were treated with S. obvelata antigens (ES-Ag, S-Ag, and ES/S-Ag) for 24 h. Optimal concentrations and time points were determined via the MTT assay. Total RNA was extracted, cDNA was synthesized, and RT-qPCR was performed using FOXP3 and RORγt primers. The gene expression fold changes and FOXP3/RORγt ratios were compared to the control group. Results showed that FOXP3 expression was increased significantly after treatment with all S. obvelata antigens, including ES-Ag, S-Ag, and ES/S-Ag, whereas the expression of RORγt decreased significantly in just two groups, including ES-Ag and ES/S-Ag. Eventually, the FOXP3/RORγt gene expression fold change ratio was increased significantly after 24 h of exposure to S. obvelata antigens. Our study indicates that the ES-Ag, S-Ag, and ES/S-Ag of S. obvelata, along with their combination, can increase the FOXP3/RORɣt ratio in PBMCs from patients with IBD, suggesting anti-inflammatory effects. This finding offers hope that these antigens could be an eye-opener for developing future strategies for IBD treatment.https://doi.org/10.1038/s41598-025-06619-0Syphacia obvelataIBDFOXP3RORɣt |
| spellingShingle | Abbas Amin Niloofar Taghipour Mohammad Rostami-Nejad Foroogh Alborzi Avanaki Reyhaneh Jafarshad Seyyed Javad Seyyed Tabaei Nariman Mosaffa Syphacia obvelata antigens alter the FOXP3/RORɣt expression balance in isolated peripheral blood mononuclear cells of IBD patients Scientific Reports Syphacia obvelata IBD FOXP3 RORɣt |
| title | Syphacia obvelata antigens alter the FOXP3/RORɣt expression balance in isolated peripheral blood mononuclear cells of IBD patients |
| title_full | Syphacia obvelata antigens alter the FOXP3/RORɣt expression balance in isolated peripheral blood mononuclear cells of IBD patients |
| title_fullStr | Syphacia obvelata antigens alter the FOXP3/RORɣt expression balance in isolated peripheral blood mononuclear cells of IBD patients |
| title_full_unstemmed | Syphacia obvelata antigens alter the FOXP3/RORɣt expression balance in isolated peripheral blood mononuclear cells of IBD patients |
| title_short | Syphacia obvelata antigens alter the FOXP3/RORɣt expression balance in isolated peripheral blood mononuclear cells of IBD patients |
| title_sort | syphacia obvelata antigens alter the foxp3 rorɣt expression balance in isolated peripheral blood mononuclear cells of ibd patients |
| topic | Syphacia obvelata IBD FOXP3 RORɣt |
| url | https://doi.org/10.1038/s41598-025-06619-0 |
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