Establishment and function of the rhizosphere fungal community in rare and endangered plant Alsophila spinulosa
IntroductionThe “rhizosphere effect” in plants occurs within approximately 5 mm from the root surface, where microbial communities exhibit distinct species composition and structural characteristics compared to non-rhizospheric soil. Root-associated fungi play crucial roles in nutrient acquisition e...
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Frontiers Media S.A.
2025-05-01
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| author | Zhang Hui-Min Zhang Hui-Min Yan Ling-Bin Yan Ling-Bin Yuan Dong-Mei Yuan Dong-Mei Liu Feng Liu Feng Cao Xiu-Gang Cao Xiu-Gang Wang Dai-Yan Wang Dai-Yan He Qin-Qin Mu Jun Yu Li-Fei Yu Li-Fei Liu Yuan Liu Yuan Chen Zhi-Fei Chen Zhi-Fei |
| author_facet | Zhang Hui-Min Zhang Hui-Min Yan Ling-Bin Yan Ling-Bin Yuan Dong-Mei Yuan Dong-Mei Liu Feng Liu Feng Cao Xiu-Gang Cao Xiu-Gang Wang Dai-Yan Wang Dai-Yan He Qin-Qin Mu Jun Yu Li-Fei Yu Li-Fei Liu Yuan Liu Yuan Chen Zhi-Fei Chen Zhi-Fei |
| author_sort | Zhang Hui-Min |
| collection | DOAJ |
| description | IntroductionThe “rhizosphere effect” in plants occurs within approximately 5 mm from the root surface, where microbial communities exhibit distinct species composition and structural characteristics compared to non-rhizospheric soil. Root-associated fungi play crucial roles in nutrient acquisition enhancement, stress resistance improvement, organic matter decomposition, and carbon cycle promotion. Current research shows limited understanding of the rhizospheric fungal communities in Alsophila spinulosa, a rare and endangered plant species.MethodsThis study conducted a comparative analysis of fungal community composition and structural differences between A. spinulosa rhizospheric and non-rhizospheric soils. The functional roles of fungi in these distinct communities were systematically analyzed, with particular emphasis on identifying keystone fungal taxa within the rhizosphere, and explained the construction process of fungal communities.ResultsThe results showed that there was no difference in α diversity between the rhizosphere and non-rhizosphere soil fungal communities of A. spinulosa, but the β diversity was significantly differentiated, indicating that the difference between rhizosphere and non-rhizosphere fungal communities was mainly reflected in species composition rather than species number. The two communities have common dominant phylum: Ascomycetes and Basidiomycetes, and common dominant genera: Mortierella and Saitozyma. The functional type was mainly saprotic. Linear discriminant analysis effect size (LEfSe) analysis revealed four biomarker genera (Arthopyrenia, Hypochnicium, Tremella, and Syncephalis) enriched in the A. spinulosa rhizospheric fungal community. Venn diagram analysis identified 169 core genera within this community, with Flavodon exclusively present in the rhizosphere. Mechanistic analysis of community assembly demonstrated that stochastic processes predominantly governed the structuring of rhizospheric fungal communities.DiscussionIn conclusion, this study elucidates the functional composition and assembly mechanisms of rhizospheric fungal communities in A. spinulosa, while identifying keystone fungal taxa potentially critical to its survival. Future investigations should: Quantify the specific contribution of Flavodon to A. spinulosa; Decipher the mechanistic linkages between these fungi and the plant’s stress resistance traits; Implement plant–soil-microbe synergistic restoration strategies to enhance natural regeneration capacity of A. spinulosa populations. |
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| spelling | doaj-art-c2ff5405ffe847e48e9601e5c4670d322025-08-20T01:54:21ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-05-011610.3389/fmicb.2025.15527481552748Establishment and function of the rhizosphere fungal community in rare and endangered plant Alsophila spinulosaZhang Hui-Min0Zhang Hui-Min1Yan Ling-Bin2Yan Ling-Bin3Yuan Dong-Mei4Yuan Dong-Mei5Liu Feng6Liu Feng7Cao Xiu-Gang8Cao Xiu-Gang9Wang Dai-Yan10Wang Dai-Yan11He Qin-Qin12Mu Jun13Yu Li-Fei14Yu Li-Fei15Liu Yuan16Liu Yuan17Chen Zhi-Fei18Chen Zhi-Fei19The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, ChinaResearch Center of Karst Ecological Environment of Guizhou University, Guiyang, ChinaThe Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, ChinaResearch Center of Karst Ecological Environment of Guizhou University, Guiyang, ChinaThe Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, ChinaResearch Center of Karst Ecological Environment of Guizhou University, Guiyang, ChinaThe Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, ChinaResearch Center of Karst Ecological Environment of Guizhou University, Guiyang, ChinaThe Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, ChinaResearch Center of Karst Ecological Environment of Guizhou University, Guiyang, ChinaThe Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, ChinaResearch Center of Karst Ecological Environment of Guizhou University, Guiyang, ChinaChishui Alsophila National Nature Reserve Administration, Chishui, ChinaGuizhou Xishui National Nature Reserve Administration, Xishui, ChinaThe Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, ChinaResearch Center of Karst Ecological Environment of Guizhou University, Guiyang, ChinaThe Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, ChinaResearch Center of Karst Ecological Environment of Guizhou University, Guiyang, ChinaThe Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, ChinaResearch Center of Karst Ecological Environment of Guizhou University, Guiyang, ChinaIntroductionThe “rhizosphere effect” in plants occurs within approximately 5 mm from the root surface, where microbial communities exhibit distinct species composition and structural characteristics compared to non-rhizospheric soil. Root-associated fungi play crucial roles in nutrient acquisition enhancement, stress resistance improvement, organic matter decomposition, and carbon cycle promotion. Current research shows limited understanding of the rhizospheric fungal communities in Alsophila spinulosa, a rare and endangered plant species.MethodsThis study conducted a comparative analysis of fungal community composition and structural differences between A. spinulosa rhizospheric and non-rhizospheric soils. The functional roles of fungi in these distinct communities were systematically analyzed, with particular emphasis on identifying keystone fungal taxa within the rhizosphere, and explained the construction process of fungal communities.ResultsThe results showed that there was no difference in α diversity between the rhizosphere and non-rhizosphere soil fungal communities of A. spinulosa, but the β diversity was significantly differentiated, indicating that the difference between rhizosphere and non-rhizosphere fungal communities was mainly reflected in species composition rather than species number. The two communities have common dominant phylum: Ascomycetes and Basidiomycetes, and common dominant genera: Mortierella and Saitozyma. The functional type was mainly saprotic. Linear discriminant analysis effect size (LEfSe) analysis revealed four biomarker genera (Arthopyrenia, Hypochnicium, Tremella, and Syncephalis) enriched in the A. spinulosa rhizospheric fungal community. Venn diagram analysis identified 169 core genera within this community, with Flavodon exclusively present in the rhizosphere. Mechanistic analysis of community assembly demonstrated that stochastic processes predominantly governed the structuring of rhizospheric fungal communities.DiscussionIn conclusion, this study elucidates the functional composition and assembly mechanisms of rhizospheric fungal communities in A. spinulosa, while identifying keystone fungal taxa potentially critical to its survival. Future investigations should: Quantify the specific contribution of Flavodon to A. spinulosa; Decipher the mechanistic linkages between these fungi and the plant’s stress resistance traits; Implement plant–soil-microbe synergistic restoration strategies to enhance natural regeneration capacity of A. spinulosa populations.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1552748/fullAlsophila spinulosafungal diversityrhizosphere fungalFUNGuildcommunity assembly |
| spellingShingle | Zhang Hui-Min Zhang Hui-Min Yan Ling-Bin Yan Ling-Bin Yuan Dong-Mei Yuan Dong-Mei Liu Feng Liu Feng Cao Xiu-Gang Cao Xiu-Gang Wang Dai-Yan Wang Dai-Yan He Qin-Qin Mu Jun Yu Li-Fei Yu Li-Fei Liu Yuan Liu Yuan Chen Zhi-Fei Chen Zhi-Fei Establishment and function of the rhizosphere fungal community in rare and endangered plant Alsophila spinulosa Frontiers in Microbiology Alsophila spinulosa fungal diversity rhizosphere fungal FUNGuild community assembly |
| title | Establishment and function of the rhizosphere fungal community in rare and endangered plant Alsophila spinulosa |
| title_full | Establishment and function of the rhizosphere fungal community in rare and endangered plant Alsophila spinulosa |
| title_fullStr | Establishment and function of the rhizosphere fungal community in rare and endangered plant Alsophila spinulosa |
| title_full_unstemmed | Establishment and function of the rhizosphere fungal community in rare and endangered plant Alsophila spinulosa |
| title_short | Establishment and function of the rhizosphere fungal community in rare and endangered plant Alsophila spinulosa |
| title_sort | establishment and function of the rhizosphere fungal community in rare and endangered plant alsophila spinulosa |
| topic | Alsophila spinulosa fungal diversity rhizosphere fungal FUNGuild community assembly |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1552748/full |
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