Colistin Resistance of Pseudomonas aeruginosa Isolated from Snakes in Taiwan

This study included fifty-eight isolates of P. aeruginosa from the oral cavity of snakes that were recruited from clinical cases, captive and wild snakes. The minimum inhibitory concentrations (MICs) for the determination of susceptibility were identified by the broth microdilution method. Polymeras...

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Main Authors: Po-Yu Liu, Ling-Ling Weng, Shu-Ying Tseng, Chou-Chen Huang, Ching-Chang Cheng, Yan-Chiao Mao, Kwong-Chung Tung
Format: Article
Language:English
Published: Wiley 2017-01-01
Series:Canadian Journal of Infectious Diseases and Medical Microbiology
Online Access:http://dx.doi.org/10.1155/2017/7058396
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author Po-Yu Liu
Ling-Ling Weng
Shu-Ying Tseng
Chou-Chen Huang
Ching-Chang Cheng
Yan-Chiao Mao
Kwong-Chung Tung
author_facet Po-Yu Liu
Ling-Ling Weng
Shu-Ying Tseng
Chou-Chen Huang
Ching-Chang Cheng
Yan-Chiao Mao
Kwong-Chung Tung
author_sort Po-Yu Liu
collection DOAJ
description This study included fifty-eight isolates of P. aeruginosa from the oral cavity of snakes that were recruited from clinical cases, captive and wild snakes. The minimum inhibitory concentrations (MICs) for the determination of susceptibility were identified by the broth microdilution method. Polymerase chain reaction (PCR) was employed to detect β-lactamases genes. With regard to antipseudomonal antibiotics, the lowest nonsusceptible rates were in aztreonam (15%), piperacillin/tazobactam (12%), and amikacin (9%). The nonsusceptible rates were high in gentamicin (33%) and colistin (55%). Meanwhile, blaTEM presented in 100% of isolates where blaAmpC, blaOXA-1, and blaOXA-10 came at 94.8%, 89.7%, and 27.6%, respectively. Emergence of multidrug resistant (MDR) strains and colistin-resistant strains highlights the potential breach of public health as P. aeruginosa could be transmitted through either direct contact or indirect dissemination through the environment. This study reports that the highly resistant P. aeruginosa from snakes’ oral cavity were discovered for the very first time in Taiwan.
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language English
publishDate 2017-01-01
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series Canadian Journal of Infectious Diseases and Medical Microbiology
spelling doaj-art-c2fdd1feaa1c4a0993fac4815c84a6692025-08-20T03:23:15ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932017-01-01201710.1155/2017/70583967058396Colistin Resistance of Pseudomonas aeruginosa Isolated from Snakes in TaiwanPo-Yu Liu0Ling-Ling Weng1Shu-Ying Tseng2Chou-Chen Huang3Ching-Chang Cheng4Yan-Chiao Mao5Kwong-Chung Tung6Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, TaiwanVeterinary Teaching Hospital, College of Veterinary Medicine, National Chiayi University, Chiayi City, TaiwanLaboratory Animal Service Center, Office of Research and Development, China Medical University, Taichung 40402, TaiwanDepartment of Emergency Medicine, Taichung Veterans General Hospital, Taichung 40402, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, TaiwanThis study included fifty-eight isolates of P. aeruginosa from the oral cavity of snakes that were recruited from clinical cases, captive and wild snakes. The minimum inhibitory concentrations (MICs) for the determination of susceptibility were identified by the broth microdilution method. Polymerase chain reaction (PCR) was employed to detect β-lactamases genes. With regard to antipseudomonal antibiotics, the lowest nonsusceptible rates were in aztreonam (15%), piperacillin/tazobactam (12%), and amikacin (9%). The nonsusceptible rates were high in gentamicin (33%) and colistin (55%). Meanwhile, blaTEM presented in 100% of isolates where blaAmpC, blaOXA-1, and blaOXA-10 came at 94.8%, 89.7%, and 27.6%, respectively. Emergence of multidrug resistant (MDR) strains and colistin-resistant strains highlights the potential breach of public health as P. aeruginosa could be transmitted through either direct contact or indirect dissemination through the environment. This study reports that the highly resistant P. aeruginosa from snakes’ oral cavity were discovered for the very first time in Taiwan.http://dx.doi.org/10.1155/2017/7058396
spellingShingle Po-Yu Liu
Ling-Ling Weng
Shu-Ying Tseng
Chou-Chen Huang
Ching-Chang Cheng
Yan-Chiao Mao
Kwong-Chung Tung
Colistin Resistance of Pseudomonas aeruginosa Isolated from Snakes in Taiwan
Canadian Journal of Infectious Diseases and Medical Microbiology
title Colistin Resistance of Pseudomonas aeruginosa Isolated from Snakes in Taiwan
title_full Colistin Resistance of Pseudomonas aeruginosa Isolated from Snakes in Taiwan
title_fullStr Colistin Resistance of Pseudomonas aeruginosa Isolated from Snakes in Taiwan
title_full_unstemmed Colistin Resistance of Pseudomonas aeruginosa Isolated from Snakes in Taiwan
title_short Colistin Resistance of Pseudomonas aeruginosa Isolated from Snakes in Taiwan
title_sort colistin resistance of pseudomonas aeruginosa isolated from snakes in taiwan
url http://dx.doi.org/10.1155/2017/7058396
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