The impact of the structural state of the tubulin cytoskeleton on chlorophyll fluorescence indicators and auxin content in Solanum tuberosum
Background. Information on the dependence of light reactions of photosynthesis on the structural state of cytoskeletal elements is extremely limited. It is known that the cytoskeleton with membranes forms a cytoskeleton-membrane complex, the disruption of which should affect the course of membran...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penza State University Publishing House
2024-11-01
|
| Series: | Известия высших учебных заведений. Поволжский регион: Естественные науки |
| Subjects: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Background. Information on the dependence of light reactions of photosynthesis
on the structural state of cytoskeletal elements is extremely limited. It is known that the cytoskeleton
with membranes forms a cytoskeleton-membrane complex, the disruption of
which should affect the course of membrane processes that are under hormonal control. It is
of interest to study the effect of antioxidants on physiological processes under conditions of
cytoskeleton destruction. Among antioxidants, researchers pay special attention to phenolic
compounds. However, there is no information in the literature about their effect on chlorophyll
fluorescence under conditions of microtubule destruction. The purpose of the work was
to study the initial and maximum fluorescenceof chlorophyll, non-photochemical quenching
of fluorescence and quantum efficiency of PS II, as well as the auxin content in Solanum
tuberosum plants with an intact and oryzalin-destructed tubulin cytoskeleton. At the same
time, the effect of the antioxidant caffeic acid on chlorophyll fluorescence during the destruction
of microtubules was studied. Materials and methods. The object of the study was potato
plants (Solanum tuberosum L.) variety Zhukovsky early, grown in soil culture. Destruction
of the tubulin cytoskeleton was carried out 15 days after emergence by spraying the plants
with a 15 μM oryzalin solution. Caffeic acid at a concentration of 0.1 mM was sprayed on
plants after treatment with oryzalin. Registration of leaf chlorophyll fluorescence in intact
plants was carried out using a MINI-PAM portable fluorimeter. Before measurements, the
leaves were dark adapted. Auxin content was determined by enzyme-linked immunosorbent
assay. Indolylacetic acid (IAA) was taken as a standard solution. Mathematical data processing
was carried out using the CXSTAT application to the Excel computer program. Results.
Studies have revealed a 22 % increase in energy losses in the antenna and from the
antenna to the PSII reaction center (F0) under the influence of oryzalin, as well as a 1.5-fold
increase in photochemical fluorescence quenching (NPQ). In this case, a 20 % decrease in
the reduction of plastoquinone in the reaction center of PS II (Fm) and a 40 % decrease in the
potential quantum efficiency (Fv/Fm) were detected. A decrease in IAA content (almost 3
times) was shown during pharmacological destruction of microtubules by oryzalin. Caffeic
acid reduced the negative effect of oryzalin on chlorophyll fluorescence and auxin content.
Disruption of the structural state of microtubules reduced the initiation of tuberization and
the productivity of potato plants. Caffeic acid removed this effect of oryzalin. Conclusions.
The dependence of Solanum tuberosum chlorophyll fluorescence indicators on the structural
state of the tubulin cytoskeleton was revealed against the background of changes in the content
of phytohormones auxins. The protective effect of the antioxidant caffeic acid on the
functioning of the photosynthetic apparatus in plants with a disturbed tubulin cytoskeleton against the background of changes in auxin content has been shown. let-membrane continuum,
and with the content of auxins. |
|---|---|
| ISSN: | 2307-9150 |