High-Performance Liquid Chromatographic Ultraviolet Determination of Memantine Hydrochloride after In Vitro Transdermal Diffusion Studies
The purpose of the present work was to validate an accurate and precise high-performance liquid chromatography (HPLC) method involving ultraviolet detection for the quantitative analysis of memantine hydrochloride. In order to analyze a molecule with no chromophoric groups that could be detected by...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/502652 |
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| author | Sergio del Rio-Sancho César E. Serna-Jiménez M. Aracely Calatayud-Pascual Cristina Balaguer-Fernández Andrés Femenía-Font Virginia Merino Alicia López-Castellano |
| author_facet | Sergio del Rio-Sancho César E. Serna-Jiménez M. Aracely Calatayud-Pascual Cristina Balaguer-Fernández Andrés Femenía-Font Virginia Merino Alicia López-Castellano |
| author_sort | Sergio del Rio-Sancho |
| collection | DOAJ |
| description | The purpose of the present work was to validate an accurate and precise high-performance liquid chromatography (HPLC) method involving ultraviolet detection for the quantitative analysis of memantine hydrochloride. In order to analyze a molecule with no chromophoric groups that could be detected by a UV/visible detector, it was necessary to extract the drug and to perform a dansylation reaction that enabled the UV/visible detection of the derivatized molecule. Separation was carried out with a 150 mm Kromasil C18 column at room temperature. The detection response, at 218 nm, was found to be linear in the concentration range from 0.5 to 50 μg/mL. The method was validated for specificity, linearity, precision, accuracy, limit of detection, limit of quantification, and robustness. The limit of detection (LOD) was 0.144 μg/mL, and the limit of quantification (LOQ) was 0.437 μg/mL. The dansylated memantine complex was stable for at least five days in all the conditions evaluated. The potential use of this method has been demonstrated by the quantification of memantine hydrochloride contained in samples from the study of its in vitro transdermal permeation. |
| format | Article |
| id | doaj-art-c2fb436503cf4054a0400e0a447b3ffb |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-c2fb436503cf4054a0400e0a447b3ffb2025-02-03T01:02:46ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/502652502652High-Performance Liquid Chromatographic Ultraviolet Determination of Memantine Hydrochloride after In Vitro Transdermal Diffusion StudiesSergio del Rio-Sancho0César E. Serna-Jiménez1M. Aracely Calatayud-Pascual2Cristina Balaguer-Fernández3Andrés Femenía-Font4Virginia Merino5Alicia López-Castellano6Departamento de Farmacia, Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Avenida Seminario s/n, Valencia, 46113 Moncada, SpainDepartamento de Farmacia, Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Avenida Seminario s/n, Valencia, 46113 Moncada, SpainDepartamento de Farmacia, Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Avenida Seminario s/n, Valencia, 46113 Moncada, SpainDepartamento de Farmacia, Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Avenida Seminario s/n, Valencia, 46113 Moncada, SpainDepartamento de Farmacia, Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Avenida Seminario s/n, Valencia, 46113 Moncada, SpainInstituto de Reconocimiento Molecular y Desarrollo Tecnológico, Centro Mixto Universidad Politécnica de Valencia, Universidad de Valencia and Departamento de Farmacia y Tecnología Farmacéutica, Universidad de Valencia, Avenida Vicente Andrés s/n, Valencia, 46100 Burjassot, SpainDepartamento de Farmacia, Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Avenida Seminario s/n, Valencia, 46113 Moncada, SpainThe purpose of the present work was to validate an accurate and precise high-performance liquid chromatography (HPLC) method involving ultraviolet detection for the quantitative analysis of memantine hydrochloride. In order to analyze a molecule with no chromophoric groups that could be detected by a UV/visible detector, it was necessary to extract the drug and to perform a dansylation reaction that enabled the UV/visible detection of the derivatized molecule. Separation was carried out with a 150 mm Kromasil C18 column at room temperature. The detection response, at 218 nm, was found to be linear in the concentration range from 0.5 to 50 μg/mL. The method was validated for specificity, linearity, precision, accuracy, limit of detection, limit of quantification, and robustness. The limit of detection (LOD) was 0.144 μg/mL, and the limit of quantification (LOQ) was 0.437 μg/mL. The dansylated memantine complex was stable for at least five days in all the conditions evaluated. The potential use of this method has been demonstrated by the quantification of memantine hydrochloride contained in samples from the study of its in vitro transdermal permeation.http://dx.doi.org/10.1155/2013/502652 |
| spellingShingle | Sergio del Rio-Sancho César E. Serna-Jiménez M. Aracely Calatayud-Pascual Cristina Balaguer-Fernández Andrés Femenía-Font Virginia Merino Alicia López-Castellano High-Performance Liquid Chromatographic Ultraviolet Determination of Memantine Hydrochloride after In Vitro Transdermal Diffusion Studies Journal of Chemistry |
| title | High-Performance Liquid Chromatographic Ultraviolet Determination of Memantine Hydrochloride after In Vitro Transdermal Diffusion Studies |
| title_full | High-Performance Liquid Chromatographic Ultraviolet Determination of Memantine Hydrochloride after In Vitro Transdermal Diffusion Studies |
| title_fullStr | High-Performance Liquid Chromatographic Ultraviolet Determination of Memantine Hydrochloride after In Vitro Transdermal Diffusion Studies |
| title_full_unstemmed | High-Performance Liquid Chromatographic Ultraviolet Determination of Memantine Hydrochloride after In Vitro Transdermal Diffusion Studies |
| title_short | High-Performance Liquid Chromatographic Ultraviolet Determination of Memantine Hydrochloride after In Vitro Transdermal Diffusion Studies |
| title_sort | high performance liquid chromatographic ultraviolet determination of memantine hydrochloride after in vitro transdermal diffusion studies |
| url | http://dx.doi.org/10.1155/2013/502652 |
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