Analysis of Bovine Serum Albumin Ligands from Puerariae flos Using Ultrafiltration Combined with HPLC-MS
Rapid screening techniques for identification of active compounds from natural products are important not only for clarification of the therapeutic material basis, but also for supplying suitable chemical markers for quality control. In the present study, ultrafiltration combined with high performan...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/648361 |
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| author | Ping Tang Shihui Si Liangliang Liu |
| author_facet | Ping Tang Shihui Si Liangliang Liu |
| author_sort | Ping Tang |
| collection | DOAJ |
| description | Rapid screening techniques for identification of active compounds from natural products are important not only for clarification of the therapeutic material basis, but also for supplying suitable chemical markers for quality control. In the present study, ultrafiltration combined with high performance liquid chromatography-mass spectrometry (HPLC-MS) was developed and conducted to screen and identify bovine serum albumin (BSA) bound ligands from Puerariae flos. Fundamental parameters affecting the screening like incubation time, BSA concentration, pH, and temperature were studied and optimized. Under the optimum conditions, nine active compounds were identified by UV and MS data. The results indicated that this method was able to screen and identify BSA bound ligands form natural products without the need of preparative isolation techniques. Moreover, the method has more effective with easier operation procedures. |
| format | Article |
| id | doaj-art-c2edef622547496d839a197d5dacd762 |
| institution | DOAJ |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-c2edef622547496d839a197d5dacd7622025-08-20T03:23:15ZengWileyJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/648361648361Analysis of Bovine Serum Albumin Ligands from Puerariae flos Using Ultrafiltration Combined with HPLC-MSPing Tang0Shihui Si1Liangliang Liu2College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, ChinaCollege of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, ChinaRapid screening techniques for identification of active compounds from natural products are important not only for clarification of the therapeutic material basis, but also for supplying suitable chemical markers for quality control. In the present study, ultrafiltration combined with high performance liquid chromatography-mass spectrometry (HPLC-MS) was developed and conducted to screen and identify bovine serum albumin (BSA) bound ligands from Puerariae flos. Fundamental parameters affecting the screening like incubation time, BSA concentration, pH, and temperature were studied and optimized. Under the optimum conditions, nine active compounds were identified by UV and MS data. The results indicated that this method was able to screen and identify BSA bound ligands form natural products without the need of preparative isolation techniques. Moreover, the method has more effective with easier operation procedures.http://dx.doi.org/10.1155/2015/648361 |
| spellingShingle | Ping Tang Shihui Si Liangliang Liu Analysis of Bovine Serum Albumin Ligands from Puerariae flos Using Ultrafiltration Combined with HPLC-MS Journal of Chemistry |
| title | Analysis of Bovine Serum Albumin Ligands from Puerariae flos Using Ultrafiltration Combined with HPLC-MS |
| title_full | Analysis of Bovine Serum Albumin Ligands from Puerariae flos Using Ultrafiltration Combined with HPLC-MS |
| title_fullStr | Analysis of Bovine Serum Albumin Ligands from Puerariae flos Using Ultrafiltration Combined with HPLC-MS |
| title_full_unstemmed | Analysis of Bovine Serum Albumin Ligands from Puerariae flos Using Ultrafiltration Combined with HPLC-MS |
| title_short | Analysis of Bovine Serum Albumin Ligands from Puerariae flos Using Ultrafiltration Combined with HPLC-MS |
| title_sort | analysis of bovine serum albumin ligands from puerariae flos using ultrafiltration combined with hplc ms |
| url | http://dx.doi.org/10.1155/2015/648361 |
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