Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia
Background: Indonesia is a tropical country with heavy rainfall, mostly in low-lying areas. Floods are one of the most common natural disasters in Indonesia, with climate change causing continuous flooding in some regions. The spread of human pathogens as a severe consequence of flooding, such as L...
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| Format: | Article |
| Language: | English |
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Tripoli University
2025-01-01
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| Series: | Open Veterinary Journal |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=225182 |
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| author | Dewi Masyithah Darlan Evita Mayasari Sunna Vyatra Hutagalung Ahadi Kurniawan Ledy Afrida Sinaga Alemina Pinem Bernike Ambarita |
| author_facet | Dewi Masyithah Darlan Evita Mayasari Sunna Vyatra Hutagalung Ahadi Kurniawan Ledy Afrida Sinaga Alemina Pinem Bernike Ambarita |
| author_sort | Dewi Masyithah Darlan |
| collection | DOAJ |
| description | Background:
Indonesia is a tropical country with heavy rainfall, mostly in low-lying areas. Floods are one of the most common natural disasters in Indonesia, with climate change causing continuous flooding in some regions. The spread of human pathogens as a severe consequence of flooding, such as Leptospira, which may cause Weil's disease, is a concern for public health.
Aim:
In this cross-sectional study, we compared DNA from the rat kidney and serum samples to identify pathogenic Leptospira using polymerase chain reaction (PCR) amplification to promote a less invasive method of collecting samples from the rat vectors.
Methods:
Fifty-nine rodents inhabiting highly populated, flood-prone suburban regions were captured inside and outside the houses. Following DNA extractions, we analyzed the quantity and quality of DNA concentration from the kidney and serum specimens using a nanophotometer. The lipL32 gene was amplified to detect the pathogenic Leptospira.
Results:
The mean value of kidney DNA was 151.67 ng/µl with an average A260/A280 value of 1.836, whereas the mean value of serum DNA was 22.08 ng/µl with an average A260/A280 value of 1.233. Twenty (33.9%) kidney DNA and 10 (16.9%) serum DNA samples showed the target DNA (lipl32). The multiple sequence alignment (MSA) analysis revealed the lipL32 sequences homology to L. interrogans ser. Copenhageni.
Conclusion:
Rat kidneys exhibited higher DNA amount and purity than the serum. Moreover, PCR detection of lipl32 revealed higher positive results in kidney DNA than serum DNA samples, with high similarity to L. interrogans lipl32 sequences. Therefore, the kidney remains a better DNA source than serum for the molecular analysis of Leptospira in rats. [Open Vet J 2025; 15(1.000): 437-445] |
| format | Article |
| id | doaj-art-c2eb91ba8c974c18a3de88a49f08e1f6 |
| institution | OA Journals |
| issn | 2226-4485 2218-6050 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj-art-c2eb91ba8c974c18a3de88a49f08e1f62025-08-20T02:11:50ZengTripoli UniversityOpen Veterinary Journal2226-44852218-60502025-01-0115143744510.5455/OVJ.2025.v15.i1.39225182Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of IndonesiaDewi Masyithah Darlan0Evita Mayasari1Sunna Vyatra Hutagalung2Ahadi Kurniawan3Ledy Afrida Sinaga4Alemina Pinem5Bernike Ambarita6Department of Parasitology, Faculty of Medicine, Universitas Sumatera Utara, Jl. Universitas No. 1 Medan 20155, North Sumatra, Indonesia Department of Microbiology, Faculty of Medicine, Universitas Sumatera Utara, Jl. Dr. Mansyur No. 5 Medan 20155, North Sumatra, Indonesia Department of Parasitology, Faculty of Medicine, Universitas Sumatera Utara, Jl. Universitas No. 1 Medan 20155, North Sumatra, Indonesia Medan Public Health Laboratory Center, Ministry of Health Republic Indonesia, Jl. K. H. Wahid Hasyim No. 15 Medan 20153, North Sumatra, Indonesia Medan Public Health Laboratory Center, Ministry of Health Republic Indonesia, Jl. K. H. Wahid Hasyim No. 15 Medan 20153, North Sumatra, Indonesia Medan Public Health Laboratory Center, Ministry of Health Republic Indonesia, Jl. K. H. Wahid Hasyim No. 15 Medan 20153, North Sumatra, Indonesia Medan Public Health Laboratory Center, Ministry of Health Republic Indonesia, Jl. K. H. Wahid Hasyim No. 15 Medan 20153, North Sumatra, IndonesiaBackground: Indonesia is a tropical country with heavy rainfall, mostly in low-lying areas. Floods are one of the most common natural disasters in Indonesia, with climate change causing continuous flooding in some regions. The spread of human pathogens as a severe consequence of flooding, such as Leptospira, which may cause Weil's disease, is a concern for public health. Aim: In this cross-sectional study, we compared DNA from the rat kidney and serum samples to identify pathogenic Leptospira using polymerase chain reaction (PCR) amplification to promote a less invasive method of collecting samples from the rat vectors. Methods: Fifty-nine rodents inhabiting highly populated, flood-prone suburban regions were captured inside and outside the houses. Following DNA extractions, we analyzed the quantity and quality of DNA concentration from the kidney and serum specimens using a nanophotometer. The lipL32 gene was amplified to detect the pathogenic Leptospira. Results: The mean value of kidney DNA was 151.67 ng/µl with an average A260/A280 value of 1.836, whereas the mean value of serum DNA was 22.08 ng/µl with an average A260/A280 value of 1.233. Twenty (33.9%) kidney DNA and 10 (16.9%) serum DNA samples showed the target DNA (lipl32). The multiple sequence alignment (MSA) analysis revealed the lipL32 sequences homology to L. interrogans ser. Copenhageni. Conclusion: Rat kidneys exhibited higher DNA amount and purity than the serum. Moreover, PCR detection of lipl32 revealed higher positive results in kidney DNA than serum DNA samples, with high similarity to L. interrogans lipl32 sequences. Therefore, the kidney remains a better DNA source than serum for the molecular analysis of Leptospira in rats. [Open Vet J 2025; 15(1.000): 437-445]http://www.ejmanager.com/fulltextpdf.php?mno=225182kidneyleptospira interroganslipl32ratserum |
| spellingShingle | Dewi Masyithah Darlan Evita Mayasari Sunna Vyatra Hutagalung Ahadi Kurniawan Ledy Afrida Sinaga Alemina Pinem Bernike Ambarita Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia Open Veterinary Journal kidney leptospira interrogans lipl32 rat serum |
| title | Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia |
| title_full | Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia |
| title_fullStr | Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia |
| title_full_unstemmed | Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia |
| title_short | Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia |
| title_sort | molecular and serological detection of leptospira interrogans among wild rats in flood prone residential areas of indonesia |
| topic | kidney leptospira interrogans lipl32 rat serum |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=225182 |
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