Silencing PPP2R1A inhibits the progression of gastric cancer cells
Abstract Background Protein phosphatase 2 regulatory subunit A alpha (PPP2R1A) is the most common scaffold protein in the PP2A complex and has known tumor-suppressive functions. However, its role in gastric cancer (GC) is still unclear. This study aims to elucidate the potential regulatory role of P...
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| Format: | Article |
| Language: | English |
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Springer
2025-04-01
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| Series: | Journal of Cancer Research and Clinical Oncology |
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| Online Access: | https://doi.org/10.1007/s00432-025-06177-y |
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| author | Gengming Cheng Laibijiang Wusiman Dingding Song Wenbin Zhang |
| author_facet | Gengming Cheng Laibijiang Wusiman Dingding Song Wenbin Zhang |
| author_sort | Gengming Cheng |
| collection | DOAJ |
| description | Abstract Background Protein phosphatase 2 regulatory subunit A alpha (PPP2R1A) is the most common scaffold protein in the PP2A complex and has known tumor-suppressive functions. However, its role in gastric cancer (GC) is still unclear. This study aims to elucidate the potential regulatory role of PPP2R1A in the biological functions of GC. Methods The mutation status and expression levels of PPP2R1A in GC were assessed through bioinformatics analysis, the correlation between PPP2R1A levels and patient survival rates was examined, and its potential functional network was analyzed. Stable AGS and MGC803 cell lines were set up for overexpressing and silencing PPP2R1A. The effects on cell proliferation, migration, invasion, and apoptosis were assessed through CCK-8 assays, scratch assays, Transwell assays, and flow cytometry. Results The expression of PPP2R1A is significantly elevated in GC samples (P < 0.001) and is not caused by mutations in PPP2R1A (P > 0.05). Patients with high levels of PPP2R1A have a poorer 5-year survival rate (P < 0.001). Silencing PPP2R1A significantly inhibits the proliferation, migration, and invasion of GC cells while promoting apoptosis (P < 0.01). In contrast, overexpression of PPP2R1A does not have a significant impact on these cellular functions (P > 0.05). Conclusion PPP2R1A has potential oncogenic properties in the progression of GC, and knocking down the expression of PPP2R1A can inhibit the tumor progression of GC cells. This suggests that PPP2R1A may serve as a potential prognostic marker and therapeutic target for GC. |
| format | Article |
| id | doaj-art-c2e59d8dd93e4df88472cf198c254ce1 |
| institution | Kabale University |
| issn | 1432-1335 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Cancer Research and Clinical Oncology |
| spelling | doaj-art-c2e59d8dd93e4df88472cf198c254ce12025-08-20T03:53:57ZengSpringerJournal of Cancer Research and Clinical Oncology1432-13352025-04-01151411210.1007/s00432-025-06177-ySilencing PPP2R1A inhibits the progression of gastric cancer cellsGengming Cheng0Laibijiang Wusiman1Dingding Song2Wenbin Zhang3Gastrointestinal Surgery Department, Xinjiang Medical University Affiliated Cancer HospitalGastrointestinal Surgery Department, Xinjiang Medical University Affiliated Cancer HospitalGastrointestinal Surgery Department, Xinjiang Medical University Affiliated Cancer HospitalGastrointestinal Surgery Department, Xinjiang Medical University Affiliated Cancer HospitalAbstract Background Protein phosphatase 2 regulatory subunit A alpha (PPP2R1A) is the most common scaffold protein in the PP2A complex and has known tumor-suppressive functions. However, its role in gastric cancer (GC) is still unclear. This study aims to elucidate the potential regulatory role of PPP2R1A in the biological functions of GC. Methods The mutation status and expression levels of PPP2R1A in GC were assessed through bioinformatics analysis, the correlation between PPP2R1A levels and patient survival rates was examined, and its potential functional network was analyzed. Stable AGS and MGC803 cell lines were set up for overexpressing and silencing PPP2R1A. The effects on cell proliferation, migration, invasion, and apoptosis were assessed through CCK-8 assays, scratch assays, Transwell assays, and flow cytometry. Results The expression of PPP2R1A is significantly elevated in GC samples (P < 0.001) and is not caused by mutations in PPP2R1A (P > 0.05). Patients with high levels of PPP2R1A have a poorer 5-year survival rate (P < 0.001). Silencing PPP2R1A significantly inhibits the proliferation, migration, and invasion of GC cells while promoting apoptosis (P < 0.01). In contrast, overexpression of PPP2R1A does not have a significant impact on these cellular functions (P > 0.05). Conclusion PPP2R1A has potential oncogenic properties in the progression of GC, and knocking down the expression of PPP2R1A can inhibit the tumor progression of GC cells. This suggests that PPP2R1A may serve as a potential prognostic marker and therapeutic target for GC.https://doi.org/10.1007/s00432-025-06177-yPPP2R1AGastric cancerCell proliferationCell migration and invasionCell apoptosis |
| spellingShingle | Gengming Cheng Laibijiang Wusiman Dingding Song Wenbin Zhang Silencing PPP2R1A inhibits the progression of gastric cancer cells Journal of Cancer Research and Clinical Oncology PPP2R1A Gastric cancer Cell proliferation Cell migration and invasion Cell apoptosis |
| title | Silencing PPP2R1A inhibits the progression of gastric cancer cells |
| title_full | Silencing PPP2R1A inhibits the progression of gastric cancer cells |
| title_fullStr | Silencing PPP2R1A inhibits the progression of gastric cancer cells |
| title_full_unstemmed | Silencing PPP2R1A inhibits the progression of gastric cancer cells |
| title_short | Silencing PPP2R1A inhibits the progression of gastric cancer cells |
| title_sort | silencing ppp2r1a inhibits the progression of gastric cancer cells |
| topic | PPP2R1A Gastric cancer Cell proliferation Cell migration and invasion Cell apoptosis |
| url | https://doi.org/10.1007/s00432-025-06177-y |
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