Investigation of the bioactivities of Saponaria mesogitana methanolic extract along with its phytochemical composition
Saponaria species are known to contain saponins which have a wide variety of biological activities. But up to now, the phenolic compounds of Saponaria mesogitana have not been clarified. Therefore, this study aimed to determine the phenolic composition and some biological activities of S. mesogitana...
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Serbian Chemical Society
2025-01-01
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| Series: | Journal of the Serbian Chemical Society |
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| Online Access: | https://doiserbia.nb.rs/img/doi/0352-5139/2025/0352-51392400088O.pdf |
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| author | Ozay Cennet Alkac Cerci Burcu Temel Aybala Pehlivan Melek |
| author_facet | Ozay Cennet Alkac Cerci Burcu Temel Aybala Pehlivan Melek |
| author_sort | Ozay Cennet |
| collection | DOAJ |
| description | Saponaria species are known to contain saponins which have a wide variety of biological activities. But up to now, the phenolic compounds of Saponaria mesogitana have not been clarified. Therefore, this study aimed to determine the phenolic composition and some biological activities of S. mesogitana for the first time. The antioxidant activities of the methanol and water extracts were assessed using the DPPH, FRAP and β-carotene/linoleic acid assays, while the total secondary metabolite content, including phenolics, flavonoids and saponins, was also determined for both extracts. Based on the antioxidant activity and total phenolic and flavonoid contents, further HPLC analysis, as well as anticancer and antimicrobial activity experiments, were conducted using the methanol extract. The anticancer potential was assessed using the MTT assay and wound healing migration test, while antibacterial activity was evaluated through disc diffusion and MIC assays. Additionally, the antibiofilm properties of the extract were examined using the crystal violet method. The methanolic extract showed high antioxidant activity, while caffeic acid and epicatechin were characterized as major phenolic compounds by HPLC. S. mesogitana inhibited not only bacterial growth but also the levels of migration of SHSY-5Y cancer cells. These findings indicate that S. mesogitana possesses potent antioxidant, anticancer, antimicrobial and antibiofilm activities associated with its bioactive phenolic constituents. |
| format | Article |
| id | doaj-art-c2e46882132f45f69219a9f7af2cace0 |
| institution | OA Journals |
| issn | 0352-5139 1820-7421 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj-art-c2e46882132f45f69219a9f7af2cace02025-08-20T02:29:59ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212025-01-0190327729010.2298/JSC240526088O0352-51392400088OInvestigation of the bioactivities of Saponaria mesogitana methanolic extract along with its phytochemical compositionOzay Cennet0https://orcid.org/0000-0002-1120-6122Alkac Cerci Burcu1https://orcid.org/0000-0002-7477-1073Temel Aybala2https://orcid.org/0000-0003-1549-7219Pehlivan Melek3https://orcid.org/0000-0001-8755-4812Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Izmir Katip Celebi University, Izmir, TürkiyeDepartment of Medical Biology, Faculty of Medicine, Izmir Katip Celebi University, Izmir, TürkiyeDepartment of Pharmaceutical Microbiology, Faculty of Pharmacy, Izmir Katip Celebi University, Izmir, TürkiyeDepartment of Medical Laboratory Techniques, Vocational School of Health Services, Izmir Katip Celebi University, Izmir, TürkiyeSaponaria species are known to contain saponins which have a wide variety of biological activities. But up to now, the phenolic compounds of Saponaria mesogitana have not been clarified. Therefore, this study aimed to determine the phenolic composition and some biological activities of S. mesogitana for the first time. The antioxidant activities of the methanol and water extracts were assessed using the DPPH, FRAP and β-carotene/linoleic acid assays, while the total secondary metabolite content, including phenolics, flavonoids and saponins, was also determined for both extracts. Based on the antioxidant activity and total phenolic and flavonoid contents, further HPLC analysis, as well as anticancer and antimicrobial activity experiments, were conducted using the methanol extract. The anticancer potential was assessed using the MTT assay and wound healing migration test, while antibacterial activity was evaluated through disc diffusion and MIC assays. Additionally, the antibiofilm properties of the extract were examined using the crystal violet method. The methanolic extract showed high antioxidant activity, while caffeic acid and epicatechin were characterized as major phenolic compounds by HPLC. S. mesogitana inhibited not only bacterial growth but also the levels of migration of SHSY-5Y cancer cells. These findings indicate that S. mesogitana possesses potent antioxidant, anticancer, antimicrobial and antibiofilm activities associated with its bioactive phenolic constituents.https://doiserbia.nb.rs/img/doi/0352-5139/2025/0352-51392400088O.pdfhplcphenolicsantioxidantcytotoxicantimigrationantibiofilm |
| spellingShingle | Ozay Cennet Alkac Cerci Burcu Temel Aybala Pehlivan Melek Investigation of the bioactivities of Saponaria mesogitana methanolic extract along with its phytochemical composition Journal of the Serbian Chemical Society hplc phenolics antioxidant cytotoxic antimigration antibiofilm |
| title | Investigation of the bioactivities of Saponaria mesogitana methanolic extract along with its phytochemical composition |
| title_full | Investigation of the bioactivities of Saponaria mesogitana methanolic extract along with its phytochemical composition |
| title_fullStr | Investigation of the bioactivities of Saponaria mesogitana methanolic extract along with its phytochemical composition |
| title_full_unstemmed | Investigation of the bioactivities of Saponaria mesogitana methanolic extract along with its phytochemical composition |
| title_short | Investigation of the bioactivities of Saponaria mesogitana methanolic extract along with its phytochemical composition |
| title_sort | investigation of the bioactivities of saponaria mesogitana methanolic extract along with its phytochemical composition |
| topic | hplc phenolics antioxidant cytotoxic antimigration antibiofilm |
| url | https://doiserbia.nb.rs/img/doi/0352-5139/2025/0352-51392400088O.pdf |
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