Somatic 'soluble' adenylyl cyclase isoforms are unaffected in Sacy tm1Lex/Sacy tm1Lex 'knockout' mice.
<h4>Background</h4>Mammalian Soluble adenylyl cyclase (sAC, Adcy10, or Sacy) represents a source of the second messenger cAMP distinct from the widely studied, G protein-regulated transmembrane adenylyl cyclases. Genetic deletion of the second through fourth coding exons in Sacy(tm1Lex)/...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2008-09-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0003251 |
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| author | Jeanne Farrell Lavoisier Ramos Martin Tresguerres Margarita Kamenetsky Lonny R Levin Jochen Buck |
| author_facet | Jeanne Farrell Lavoisier Ramos Martin Tresguerres Margarita Kamenetsky Lonny R Levin Jochen Buck |
| author_sort | Jeanne Farrell |
| collection | DOAJ |
| description | <h4>Background</h4>Mammalian Soluble adenylyl cyclase (sAC, Adcy10, or Sacy) represents a source of the second messenger cAMP distinct from the widely studied, G protein-regulated transmembrane adenylyl cyclases. Genetic deletion of the second through fourth coding exons in Sacy(tm1Lex)/Sacy(tm1Lex) knockout mice results in a male sterile phenotype. The absence of any major somatic phenotype is inconsistent with the variety of somatic functions identified for sAC using pharmacological inhibitors and RNA interference.<h4>Principal findings</h4>We now use immunological and molecular biological methods to demonstrate that somatic tissues express a previously unknown isoform of sAC, which utilizes a unique start site, and which 'escapes' the design of the Sacy(tm1Lex) knockout allele.<h4>Conclusions/significance</h4>These studies reveal increased complexity at the sAC locus, and they suggest that the known isoforms of sAC play a unique function in male germ cells. |
| format | Article |
| id | doaj-art-c2df02c708ae44928649b122d1d5d41e |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2008-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-c2df02c708ae44928649b122d1d5d41e2025-08-20T02:38:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-09-0139e325110.1371/journal.pone.0003251Somatic 'soluble' adenylyl cyclase isoforms are unaffected in Sacy tm1Lex/Sacy tm1Lex 'knockout' mice.Jeanne FarrellLavoisier RamosMartin TresguerresMargarita KamenetskyLonny R LevinJochen Buck<h4>Background</h4>Mammalian Soluble adenylyl cyclase (sAC, Adcy10, or Sacy) represents a source of the second messenger cAMP distinct from the widely studied, G protein-regulated transmembrane adenylyl cyclases. Genetic deletion of the second through fourth coding exons in Sacy(tm1Lex)/Sacy(tm1Lex) knockout mice results in a male sterile phenotype. The absence of any major somatic phenotype is inconsistent with the variety of somatic functions identified for sAC using pharmacological inhibitors and RNA interference.<h4>Principal findings</h4>We now use immunological and molecular biological methods to demonstrate that somatic tissues express a previously unknown isoform of sAC, which utilizes a unique start site, and which 'escapes' the design of the Sacy(tm1Lex) knockout allele.<h4>Conclusions/significance</h4>These studies reveal increased complexity at the sAC locus, and they suggest that the known isoforms of sAC play a unique function in male germ cells.https://doi.org/10.1371/journal.pone.0003251 |
| spellingShingle | Jeanne Farrell Lavoisier Ramos Martin Tresguerres Margarita Kamenetsky Lonny R Levin Jochen Buck Somatic 'soluble' adenylyl cyclase isoforms are unaffected in Sacy tm1Lex/Sacy tm1Lex 'knockout' mice. PLoS ONE |
| title | Somatic 'soluble' adenylyl cyclase isoforms are unaffected in Sacy tm1Lex/Sacy tm1Lex 'knockout' mice. |
| title_full | Somatic 'soluble' adenylyl cyclase isoforms are unaffected in Sacy tm1Lex/Sacy tm1Lex 'knockout' mice. |
| title_fullStr | Somatic 'soluble' adenylyl cyclase isoforms are unaffected in Sacy tm1Lex/Sacy tm1Lex 'knockout' mice. |
| title_full_unstemmed | Somatic 'soluble' adenylyl cyclase isoforms are unaffected in Sacy tm1Lex/Sacy tm1Lex 'knockout' mice. |
| title_short | Somatic 'soluble' adenylyl cyclase isoforms are unaffected in Sacy tm1Lex/Sacy tm1Lex 'knockout' mice. |
| title_sort | somatic soluble adenylyl cyclase isoforms are unaffected in sacy tm1lex sacy tm1lex knockout mice |
| url | https://doi.org/10.1371/journal.pone.0003251 |
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