PpHSP20-26, a small heat shock protein, confers enhanced autotoxicity stress tolerance in peach
Autotoxicity stress is the principal factor in peach replant problem. Benzoic acid (BA) is known as a critical autotoxin in replant problem, and causes an obvious inhibitory effect on peach growth. Small heat shock proteins (sHSPs) have been reported to play pivotal roles in a variety of physiologic...
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KeAi Communications Co., Ltd.
2025-05-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014124001481 |
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| author | Wanqi Shen Chunfa Zeng Jingxian Sun Jian Meng Ping Yuan Fanwen Bu Kaijie Zhu Junwei Liu Guohuai Li |
| author_facet | Wanqi Shen Chunfa Zeng Jingxian Sun Jian Meng Ping Yuan Fanwen Bu Kaijie Zhu Junwei Liu Guohuai Li |
| author_sort | Wanqi Shen |
| collection | DOAJ |
| description | Autotoxicity stress is the principal factor in peach replant problem. Benzoic acid (BA) is known as a critical autotoxin in replant problem, and causes an obvious inhibitory effect on peach growth. Small heat shock proteins (sHSPs) have been reported to play pivotal roles in a variety of physiological and biological processes in various plants. Nevertheless, little is known about the functions and the underlying physiological mechanisms of sHSPs under autotoxicity stress. Here, we identified PpHSP20-26 of peach (Prunus persica) and deciphered its role in BA stress response. PpHSP20-26 was significantly induced by BA treatment. Overexpression of PpHSP20-26 elevated BA tolerance in Arabidopsis and peach plants, whereas down-regulation of PpHSP20-26 in peach through virus-induced gene silencing enhanced BA sensitivity. Compared to the control, the PpHSP20-26-overexpressing plants exhibited lower contents of reactive oxygen species (ROS) and higher activities of antioxidant enzymes. Furthermore, PpHSP20-26 regulated the transcripts of stress-responsive genes including CAT, SOD, APX, GPX, DHAR, and ABC transporters in overexpressing Arabidopsis and silenced peach plants. Taken together, these data suggest that PpHSP20-26 plays a positive role in peach response to BA stress by, at least partly, regulating ROS metabolism and stress-responsive gene expression. Our findings will be of great importance for further understanding the roles of sHSPs genes in autotoxicity stress, and assist crop breeding in mitigating replant problem. |
| format | Article |
| id | doaj-art-c2cda08443ec4e91a6a22721da98aed0 |
| institution | DOAJ |
| issn | 2468-0141 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj-art-c2cda08443ec4e91a6a22721da98aed02025-08-20T03:14:32ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412025-05-011131012102510.1016/j.hpj.2024.01.011PpHSP20-26, a small heat shock protein, confers enhanced autotoxicity stress tolerance in peachWanqi Shen0Chunfa Zeng1Jingxian Sun2Jian Meng3Ping Yuan4Fanwen Bu5Kaijie Zhu6Junwei Liu7Guohuai Li8National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Hunan Horticulture Research Institute, Hunan Academy of Agricultural Sciences, Changsha, Hunan 410125, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaHunan Horticulture Research Institute, Hunan Academy of Agricultural Sciences, Changsha, Hunan 410125, ChinaHunan Horticulture Research Institute, Hunan Academy of Agricultural Sciences, Changsha, Hunan 410125, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Corresponding authors.National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Corresponding authors.National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaAutotoxicity stress is the principal factor in peach replant problem. Benzoic acid (BA) is known as a critical autotoxin in replant problem, and causes an obvious inhibitory effect on peach growth. Small heat shock proteins (sHSPs) have been reported to play pivotal roles in a variety of physiological and biological processes in various plants. Nevertheless, little is known about the functions and the underlying physiological mechanisms of sHSPs under autotoxicity stress. Here, we identified PpHSP20-26 of peach (Prunus persica) and deciphered its role in BA stress response. PpHSP20-26 was significantly induced by BA treatment. Overexpression of PpHSP20-26 elevated BA tolerance in Arabidopsis and peach plants, whereas down-regulation of PpHSP20-26 in peach through virus-induced gene silencing enhanced BA sensitivity. Compared to the control, the PpHSP20-26-overexpressing plants exhibited lower contents of reactive oxygen species (ROS) and higher activities of antioxidant enzymes. Furthermore, PpHSP20-26 regulated the transcripts of stress-responsive genes including CAT, SOD, APX, GPX, DHAR, and ABC transporters in overexpressing Arabidopsis and silenced peach plants. Taken together, these data suggest that PpHSP20-26 plays a positive role in peach response to BA stress by, at least partly, regulating ROS metabolism and stress-responsive gene expression. Our findings will be of great importance for further understanding the roles of sHSPs genes in autotoxicity stress, and assist crop breeding in mitigating replant problem.http://www.sciencedirect.com/science/article/pii/S2468014124001481Plant autotoxicityBenzoic acidPrunus persicaReplant problemStress response |
| spellingShingle | Wanqi Shen Chunfa Zeng Jingxian Sun Jian Meng Ping Yuan Fanwen Bu Kaijie Zhu Junwei Liu Guohuai Li PpHSP20-26, a small heat shock protein, confers enhanced autotoxicity stress tolerance in peach Horticultural Plant Journal Plant autotoxicity Benzoic acid Prunus persica Replant problem Stress response |
| title | PpHSP20-26, a small heat shock protein, confers enhanced autotoxicity stress tolerance in peach |
| title_full | PpHSP20-26, a small heat shock protein, confers enhanced autotoxicity stress tolerance in peach |
| title_fullStr | PpHSP20-26, a small heat shock protein, confers enhanced autotoxicity stress tolerance in peach |
| title_full_unstemmed | PpHSP20-26, a small heat shock protein, confers enhanced autotoxicity stress tolerance in peach |
| title_short | PpHSP20-26, a small heat shock protein, confers enhanced autotoxicity stress tolerance in peach |
| title_sort | pphsp20 26 a small heat shock protein confers enhanced autotoxicity stress tolerance in peach |
| topic | Plant autotoxicity Benzoic acid Prunus persica Replant problem Stress response |
| url | http://www.sciencedirect.com/science/article/pii/S2468014124001481 |
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