Media Optimization for Amylase Production through OFAT Using Onion Peel as Substrate
Amylase is a vital enzyme with extensive industrial applications, including in food processing, textiles, and biofuels. This study explores a novel and sustainable approach to amylase production using Bacillus subtilis and onion peel, an agro-industrial waste product, as a substrate by using OFAT(On...
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EDP Sciences
2025-01-01
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| Series: | BIO Web of Conferences |
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| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2025/29/bioconf_amifost2025_03005.pdf |
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| author | Zahid Anila Khedkar Renu D. Sharma Shashi Rajput Sanjay |
| author_facet | Zahid Anila Khedkar Renu D. Sharma Shashi Rajput Sanjay |
| author_sort | Zahid Anila |
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| description | Amylase is a vital enzyme with extensive industrial applications, including in food processing, textiles, and biofuels. This study explores a novel and sustainable approach to amylase production using Bacillus subtilis and onion peel, an agro-industrial waste product, as a substrate by using OFAT(One-Factor-At-a-Time) technique. Onion peel, rich in carbohydrates and nutrients, was utilized to enhance enzymatic activity while reducing production costs and promoting waste valorization. A systematic optimization of media components, including substrate concentration, nitrogen source, pH, and temperature, was performed to maximize bacterial growth, enzyme activity, and protein production. The optimized conditions—15 g/L substrate concentration, peptone (2 g/L) as the nitrogen source, neutral pH (pH 7), and a temperature of 37°C yielded the highest bacterial growth (OD 1.00), enzyme activity (0.38 μmol/min), and protein concentration (0.60 mg/ml). Purification of the enzyme using 40% ammonium sulfate precipitation achieved a twofold increase in specific activity (40 U/mg) with an 80% yield, demonstrating the effectiveness of the purification process. This research highlights the potential of onion peel as a sustainable and cost-effective substrate for microbial enzyme production. The findings not only contribute to advancing enzyme production processes but also align with global efforts to adopt eco-friendly and sustainable industrial practices. The study offers a scalable framework for enhancing amylase production and paves the way for its application in diverse industrial sectors. |
| format | Article |
| id | doaj-art-c2c73d14c97e499a9f511b3381f40a86 |
| institution | OA Journals |
| issn | 2117-4458 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
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| series | BIO Web of Conferences |
| spelling | doaj-art-c2c73d14c97e499a9f511b3381f40a862025-08-20T02:05:51ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011780300510.1051/bioconf/202517803005bioconf_amifost2025_03005Media Optimization for Amylase Production through OFAT Using Onion Peel as SubstrateZahid Anila0Khedkar Renu D.1Sharma Shashi2Rajput Sanjay3Amity Institute of Food Technology, Amity UniversityAmity Institute of Food Technology, Amity UniversityAmity Institute of Bio-Technology, Amity UniversityShriram Applied Radiation Centre, Shriram Institute for Industrial ResearchAmylase is a vital enzyme with extensive industrial applications, including in food processing, textiles, and biofuels. This study explores a novel and sustainable approach to amylase production using Bacillus subtilis and onion peel, an agro-industrial waste product, as a substrate by using OFAT(One-Factor-At-a-Time) technique. Onion peel, rich in carbohydrates and nutrients, was utilized to enhance enzymatic activity while reducing production costs and promoting waste valorization. A systematic optimization of media components, including substrate concentration, nitrogen source, pH, and temperature, was performed to maximize bacterial growth, enzyme activity, and protein production. The optimized conditions—15 g/L substrate concentration, peptone (2 g/L) as the nitrogen source, neutral pH (pH 7), and a temperature of 37°C yielded the highest bacterial growth (OD 1.00), enzyme activity (0.38 μmol/min), and protein concentration (0.60 mg/ml). Purification of the enzyme using 40% ammonium sulfate precipitation achieved a twofold increase in specific activity (40 U/mg) with an 80% yield, demonstrating the effectiveness of the purification process. This research highlights the potential of onion peel as a sustainable and cost-effective substrate for microbial enzyme production. The findings not only contribute to advancing enzyme production processes but also align with global efforts to adopt eco-friendly and sustainable industrial practices. The study offers a scalable framework for enhancing amylase production and paves the way for its application in diverse industrial sectors.https://www.bio-conferences.org/articles/bioconf/pdf/2025/29/bioconf_amifost2025_03005.pdfamylasebacillus subtilisofatonion peelmedia optimization |
| spellingShingle | Zahid Anila Khedkar Renu D. Sharma Shashi Rajput Sanjay Media Optimization for Amylase Production through OFAT Using Onion Peel as Substrate BIO Web of Conferences amylase bacillus subtilis ofat onion peel media optimization |
| title | Media Optimization for Amylase Production through OFAT Using Onion Peel as Substrate |
| title_full | Media Optimization for Amylase Production through OFAT Using Onion Peel as Substrate |
| title_fullStr | Media Optimization for Amylase Production through OFAT Using Onion Peel as Substrate |
| title_full_unstemmed | Media Optimization for Amylase Production through OFAT Using Onion Peel as Substrate |
| title_short | Media Optimization for Amylase Production through OFAT Using Onion Peel as Substrate |
| title_sort | media optimization for amylase production through ofat using onion peel as substrate |
| topic | amylase bacillus subtilis ofat onion peel media optimization |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2025/29/bioconf_amifost2025_03005.pdf |
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