Protective effect and antioxidative efficacy of Moringa stenopetala leaf extract in BV-2 microglial cell
Moringa stenopetala is often consumed as food and used in folkloric medicine for the management of several diseases including neurodegenerative diseases. This study investigated the protective effect and antioxidant efficacy of aqueous extract of Moringa stenopetala in BV-2 microglial cells. Aqueous...
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Elsevier
2024-12-01
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| Series: | Food Chemistry Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772753X24001631 |
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| author | Stephen Adeniyi Adefegha Vitor Mostardeiro Vera Maria Morsch Ademir F. Morel Ivana Beatrice Manica Da Cruz Sabrina Somacal Ganiyu Oboh Maria Rosa Chitolina Schetinger |
| author_facet | Stephen Adeniyi Adefegha Vitor Mostardeiro Vera Maria Morsch Ademir F. Morel Ivana Beatrice Manica Da Cruz Sabrina Somacal Ganiyu Oboh Maria Rosa Chitolina Schetinger |
| author_sort | Stephen Adeniyi Adefegha |
| collection | DOAJ |
| description | Moringa stenopetala is often consumed as food and used in folkloric medicine for the management of several diseases including neurodegenerative diseases. This study investigated the protective effect and antioxidant efficacy of aqueous extract of Moringa stenopetala in BV-2 microglial cells. Aqueous extracts of M. stenopetala were prepared, lyophilized and reconstituted in 0.5% dimethylsulphoxide (DMSO). Cells were treated with M. stenopetala extracts (0.1–100 µg/ml) for cell viability assay and nitric oxide (NO) production tests. However, M. stenopetala extract (50 µg/ml) was used in the treatment of cells for the determination of protein carbonyl content and reactive oxygen species (ROS) level. Incubation of BV-2 microglia cell with M. stenopetala extract maintained cell viability, diminished NO and ROS levels, and reduced protein carbonyl contents. Chlorogenic acid, rutin, kaempferol and quercetin derivatives were the main phenolic compounds identified in M. stenopetala leaf extract. In conclusion, this study suggests that M. stenopetala extract is rich in antioxidant and contains bioactive neuroprotective agents. |
| format | Article |
| id | doaj-art-c2bfeab8e03e4bc698ed58328d79681a |
| institution | OA Journals |
| issn | 2772-753X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Food Chemistry Advances |
| spelling | doaj-art-c2bfeab8e03e4bc698ed58328d79681a2025-08-20T01:59:35ZengElsevierFood Chemistry Advances2772-753X2024-12-01510076710.1016/j.focha.2024.100767Protective effect and antioxidative efficacy of Moringa stenopetala leaf extract in BV-2 microglial cellStephen Adeniyi Adefegha0Vitor Mostardeiro1Vera Maria Morsch2Ademir F. Morel3Ivana Beatrice Manica Da Cruz4Sabrina Somacal5Ganiyu Oboh6Maria Rosa Chitolina Schetinger7Functional Foods, Nutraceuticals and Phytomedicine Laboratory, Department of Biochemistry, Federal University of Technology, P. M. B. 704 Akure, Nigeria; Graduate Program in Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Center of Natural and Exacts Sciences, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil; Corresponding author.Graduate Program in Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Center of Natural and Exacts Sciences, Federal University of Santa Maria, Santa Maria, RS 97105-900, BrazilGraduate Program in Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Center of Natural and Exacts Sciences, Federal University of Santa Maria, Santa Maria, RS 97105-900, BrazilDepartment of Chemistry, Center of Natural and Exacts Sciences, Federal University of Santa Maria, RS 97105-900, BrazilBiogenomics Laboratory, Department of Morphology, Federal University of Santa Maria, RS 97105-900, BrazilDepartment of Food Science and Technology, Center for Rural Sciences, Federal University of Santa Maria, RS 97105-900, BrazilFunctional Foods, Nutraceuticals and Phytomedicine Laboratory, Department of Biochemistry, Federal University of Technology, P. M. B. 704 Akure, Nigeria; Graduate Program in Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Center of Natural and Exacts Sciences, Federal University of Santa Maria, Santa Maria, RS 97105-900, BrazilGraduate Program in Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Center of Natural and Exacts Sciences, Federal University of Santa Maria, Santa Maria, RS 97105-900, BrazilMoringa stenopetala is often consumed as food and used in folkloric medicine for the management of several diseases including neurodegenerative diseases. This study investigated the protective effect and antioxidant efficacy of aqueous extract of Moringa stenopetala in BV-2 microglial cells. Aqueous extracts of M. stenopetala were prepared, lyophilized and reconstituted in 0.5% dimethylsulphoxide (DMSO). Cells were treated with M. stenopetala extracts (0.1–100 µg/ml) for cell viability assay and nitric oxide (NO) production tests. However, M. stenopetala extract (50 µg/ml) was used in the treatment of cells for the determination of protein carbonyl content and reactive oxygen species (ROS) level. Incubation of BV-2 microglia cell with M. stenopetala extract maintained cell viability, diminished NO and ROS levels, and reduced protein carbonyl contents. Chlorogenic acid, rutin, kaempferol and quercetin derivatives were the main phenolic compounds identified in M. stenopetala leaf extract. In conclusion, this study suggests that M. stenopetala extract is rich in antioxidant and contains bioactive neuroprotective agents.http://www.sciencedirect.com/science/article/pii/S2772753X24001631Moringa stenopetalaCell viabilityOxidative stressAntioxidantBV-2 microglial cell |
| spellingShingle | Stephen Adeniyi Adefegha Vitor Mostardeiro Vera Maria Morsch Ademir F. Morel Ivana Beatrice Manica Da Cruz Sabrina Somacal Ganiyu Oboh Maria Rosa Chitolina Schetinger Protective effect and antioxidative efficacy of Moringa stenopetala leaf extract in BV-2 microglial cell Food Chemistry Advances Moringa stenopetala Cell viability Oxidative stress Antioxidant BV-2 microglial cell |
| title | Protective effect and antioxidative efficacy of Moringa stenopetala leaf extract in BV-2 microglial cell |
| title_full | Protective effect and antioxidative efficacy of Moringa stenopetala leaf extract in BV-2 microglial cell |
| title_fullStr | Protective effect and antioxidative efficacy of Moringa stenopetala leaf extract in BV-2 microglial cell |
| title_full_unstemmed | Protective effect and antioxidative efficacy of Moringa stenopetala leaf extract in BV-2 microglial cell |
| title_short | Protective effect and antioxidative efficacy of Moringa stenopetala leaf extract in BV-2 microglial cell |
| title_sort | protective effect and antioxidative efficacy of moringa stenopetala leaf extract in bv 2 microglial cell |
| topic | Moringa stenopetala Cell viability Oxidative stress Antioxidant BV-2 microglial cell |
| url | http://www.sciencedirect.com/science/article/pii/S2772753X24001631 |
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