Competitive antagonistic action of laccase between Trichoderma species and the newly identified wood pathogenic Ganoderma camelum
Ganoderma, a well-known genus in the Ganodermataceae family, has caused the extinction of several tree species due to its pathogenicity. This study explored the pathogenic effect of a newly identified Ganoderma species on trees and its competitive efficiency against Trichoderma species. Ganoderma ca...
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Frontiers Media S.A.
2024-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1408521/full |
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| author | Aisha Umar Aisha Umar Mohamed S. Elshikh Reem M. Aljowaie Juma Mahmud Hussein Laurent Dufossé Chenghong Wu Junxing Lu |
| author_facet | Aisha Umar Aisha Umar Mohamed S. Elshikh Reem M. Aljowaie Juma Mahmud Hussein Laurent Dufossé Chenghong Wu Junxing Lu |
| author_sort | Aisha Umar |
| collection | DOAJ |
| description | Ganoderma, a well-known genus in the Ganodermataceae family, has caused the extinction of several tree species due to its pathogenicity. This study explored the pathogenic effect of a newly identified Ganoderma species on trees and its competitive efficiency against Trichoderma species. Ganoderma camelum sp. nov. is characterized by small sessile basidiomata and a velvety, soft, camel-brown pileus. Phylogenetic analysis and ITS rDNA sequences indicated that the species were Trichoderma and Ganoderma camelum. Both fungal species competed antagonistically by secreting laccase. The laccase activity of G. camelum, with a value of 8.3 ± 4.0 U/mL, demonstrated the highest competitive activity against Trichoderma species. The laccase produced by T. atroviride (2.62 U/mL) was most effective in countering the pathogenic action of the novel G. camelum. The molecular weights of laccase were determined using SDS-PAGE (62.0 kDa for G. camelum and 57.0 kDa for T. atroviride). Due to the white rot induced by this Ganoderma species in the host tree, G. camelum showed the highest percentage inhibition of radial growth (76.3%) compared to T. atroviride (28.7%). This study aimed to evaluate the competitive antagonistic activity of Ganoderma and Trichoderma on malt extract agar media in the context of white rot disease in the host tree. This study concluded that the laccase from G. camelum caused weight loss in rubber wood blocks through laccase action, indicating tissue injury in the host species. Therefore, it was also concluded that G. camelum was more effective in pathogenic action of the host and resisted the biological action of T. atroviride. In principal components analysis (PCA), all the species associated with laccase exhibited a very strong influence on the variability of the system. The PIRG rate (percentage inhibition of radial growth) was strongly and positively correlated with laccase activity. |
| format | Article |
| id | doaj-art-c2bfd51645834fd5830adf5e8e23c412 |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-c2bfd51645834fd5830adf5e8e23c4122025-08-20T01:54:44ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-09-011510.3389/fmicb.2024.14085211408521Competitive antagonistic action of laccase between Trichoderma species and the newly identified wood pathogenic Ganoderma camelumAisha Umar0Aisha Umar1Mohamed S. Elshikh2Reem M. Aljowaie3Juma Mahmud Hussein4Laurent Dufossé5Chenghong Wu6Junxing Lu7Chongqing Key Laboratory of Plant Environmental Adaptations, College of Life Science, Chongqing Normal University, Chongqing, ChinaInstitute of Botany, University of the Punjab, Lahore, PakistanDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Molecular Biology and Biotechnology, University of Dar es Salaam, Dar es Salaam, TanzaniaCHEMBIOPRO Laboratoire de Chimie et Biotechnologie des Produits Naturels, ESIROI Agroalimentaire, Université de La Réunion, Saint-Denis, Ile de La Réunion, FranceChongqing Key Laboratory of Plant Environmental Adaptations, College of Life Science, Chongqing Normal University, Chongqing, ChinaChongqing Key Laboratory of Plant Environmental Adaptations, College of Life Science, Chongqing Normal University, Chongqing, ChinaGanoderma, a well-known genus in the Ganodermataceae family, has caused the extinction of several tree species due to its pathogenicity. This study explored the pathogenic effect of a newly identified Ganoderma species on trees and its competitive efficiency against Trichoderma species. Ganoderma camelum sp. nov. is characterized by small sessile basidiomata and a velvety, soft, camel-brown pileus. Phylogenetic analysis and ITS rDNA sequences indicated that the species were Trichoderma and Ganoderma camelum. Both fungal species competed antagonistically by secreting laccase. The laccase activity of G. camelum, with a value of 8.3 ± 4.0 U/mL, demonstrated the highest competitive activity against Trichoderma species. The laccase produced by T. atroviride (2.62 U/mL) was most effective in countering the pathogenic action of the novel G. camelum. The molecular weights of laccase were determined using SDS-PAGE (62.0 kDa for G. camelum and 57.0 kDa for T. atroviride). Due to the white rot induced by this Ganoderma species in the host tree, G. camelum showed the highest percentage inhibition of radial growth (76.3%) compared to T. atroviride (28.7%). This study aimed to evaluate the competitive antagonistic activity of Ganoderma and Trichoderma on malt extract agar media in the context of white rot disease in the host tree. This study concluded that the laccase from G. camelum caused weight loss in rubber wood blocks through laccase action, indicating tissue injury in the host species. Therefore, it was also concluded that G. camelum was more effective in pathogenic action of the host and resisted the biological action of T. atroviride. In principal components analysis (PCA), all the species associated with laccase exhibited a very strong influence on the variability of the system. The PIRG rate (percentage inhibition of radial growth) was strongly and positively correlated with laccase activity.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1408521/fullgrowth rateITSlaccasephylogenywood degradation |
| spellingShingle | Aisha Umar Aisha Umar Mohamed S. Elshikh Reem M. Aljowaie Juma Mahmud Hussein Laurent Dufossé Chenghong Wu Junxing Lu Competitive antagonistic action of laccase between Trichoderma species and the newly identified wood pathogenic Ganoderma camelum Frontiers in Microbiology growth rate ITS laccase phylogeny wood degradation |
| title | Competitive antagonistic action of laccase between Trichoderma species and the newly identified wood pathogenic Ganoderma camelum |
| title_full | Competitive antagonistic action of laccase between Trichoderma species and the newly identified wood pathogenic Ganoderma camelum |
| title_fullStr | Competitive antagonistic action of laccase between Trichoderma species and the newly identified wood pathogenic Ganoderma camelum |
| title_full_unstemmed | Competitive antagonistic action of laccase between Trichoderma species and the newly identified wood pathogenic Ganoderma camelum |
| title_short | Competitive antagonistic action of laccase between Trichoderma species and the newly identified wood pathogenic Ganoderma camelum |
| title_sort | competitive antagonistic action of laccase between trichoderma species and the newly identified wood pathogenic ganoderma camelum |
| topic | growth rate ITS laccase phylogeny wood degradation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1408521/full |
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