Design and preparation of PLA-chitosan-PEG-glucose copolymer for combined delivery of Paclitaxel and siRNA
Abstract In recent years, the use of cell surface receptors for targeted nanoparticle delivery to cancer cells has emerged as a new strategy that enhances drug effectiveness by minimizing nonspecific absorption in healthy cells and reducing side effects. This study focused on dual-purpose chitosan-p...
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| Format: | Article |
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Springer
2025-07-01
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| Series: | Discover Applied Sciences |
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| Online Access: | https://doi.org/10.1007/s42452-025-07458-4 |
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| author | Sahar Mohajeri Shalaleh Dashti Mehran Noruzpour Shima Bourang Hashem Yaghoubi |
| author_facet | Sahar Mohajeri Shalaleh Dashti Mehran Noruzpour Shima Bourang Hashem Yaghoubi |
| author_sort | Sahar Mohajeri |
| collection | DOAJ |
| description | Abstract In recent years, the use of cell surface receptors for targeted nanoparticle delivery to cancer cells has emerged as a new strategy that enhances drug effectiveness by minimizing nonspecific absorption in healthy cells and reducing side effects. This study focused on dual-purpose chitosan-polylactic acid-polyethylene glycol (PLA-PEG) nanoparticles, which target glucose to improve the delivery of Paclitaxel (PTX) and small interfering RNA-fluorescein amidite (siRNA-FAM) to cancer cells. The PLA-chitosan-PEG-glucose (Glu) copolymer was synthesized by binding chitosan-PLA and PEG-Glu, and then was characterized via Fourier transform infrared (FTIR) spectroscopy and proton nuclear magnetic resonance (1H-NMR) spectroscopy, confirming successful synthesis. The FTIR results indicated successful copolymer formation. The solvent diffusion technique involves the encapsulation of PTX and siRNA-FAM within the nanoparticles. Scanning electron microscopy (SEM) and dynamic light scattering (DLS) were employed to analyze the morphology, size, and surface charge of the nanoparticles. SEM images revealed that the nanoparticles had a spherical structure, and DLS analysis indicated an average size of approximately 188 nm. The biocompatibility of the nanoparticles was assessed via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, and their drug transport ability was evaluated via flow cytometry. The results demonstrated that the PLA-chitosan-PEG-Glu copolymer exhibited low toxicity due to the combination of PLA and PEG, while the efficiency of siRNA-FAM transport was significantly greater than that of uncoated RNA. Graphical abstract |
| format | Article |
| id | doaj-art-c2be96cfc34b48c494b03a0c305eba56 |
| institution | Kabale University |
| issn | 3004-9261 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Applied Sciences |
| spelling | doaj-art-c2be96cfc34b48c494b03a0c305eba562025-08-20T04:03:07ZengSpringerDiscover Applied Sciences3004-92612025-07-017812610.1007/s42452-025-07458-4Design and preparation of PLA-chitosan-PEG-glucose copolymer for combined delivery of Paclitaxel and siRNASahar Mohajeri0Shalaleh Dashti1Mehran Noruzpour2Shima Bourang3Hashem Yaghoubi4Department of Chemistry, Ard.C., Islamic Azad UniversityDepartment of Biology, Ard.C., Islamic Azad UniversityDepartment of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh ArdabiliDepartment of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh ArdabiliDepartment of Biology, Ard.C., Islamic Azad UniversityAbstract In recent years, the use of cell surface receptors for targeted nanoparticle delivery to cancer cells has emerged as a new strategy that enhances drug effectiveness by minimizing nonspecific absorption in healthy cells and reducing side effects. This study focused on dual-purpose chitosan-polylactic acid-polyethylene glycol (PLA-PEG) nanoparticles, which target glucose to improve the delivery of Paclitaxel (PTX) and small interfering RNA-fluorescein amidite (siRNA-FAM) to cancer cells. The PLA-chitosan-PEG-glucose (Glu) copolymer was synthesized by binding chitosan-PLA and PEG-Glu, and then was characterized via Fourier transform infrared (FTIR) spectroscopy and proton nuclear magnetic resonance (1H-NMR) spectroscopy, confirming successful synthesis. The FTIR results indicated successful copolymer formation. The solvent diffusion technique involves the encapsulation of PTX and siRNA-FAM within the nanoparticles. Scanning electron microscopy (SEM) and dynamic light scattering (DLS) were employed to analyze the morphology, size, and surface charge of the nanoparticles. SEM images revealed that the nanoparticles had a spherical structure, and DLS analysis indicated an average size of approximately 188 nm. The biocompatibility of the nanoparticles was assessed via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, and their drug transport ability was evaluated via flow cytometry. The results demonstrated that the PLA-chitosan-PEG-Glu copolymer exhibited low toxicity due to the combination of PLA and PEG, while the efficiency of siRNA-FAM transport was significantly greater than that of uncoated RNA. Graphical abstracthttps://doi.org/10.1007/s42452-025-07458-4BiocompatibilityCancer therapyDrug deliveryNanoparticle targetingPTXTransfection |
| spellingShingle | Sahar Mohajeri Shalaleh Dashti Mehran Noruzpour Shima Bourang Hashem Yaghoubi Design and preparation of PLA-chitosan-PEG-glucose copolymer for combined delivery of Paclitaxel and siRNA Discover Applied Sciences Biocompatibility Cancer therapy Drug delivery Nanoparticle targeting PTX Transfection |
| title | Design and preparation of PLA-chitosan-PEG-glucose copolymer for combined delivery of Paclitaxel and siRNA |
| title_full | Design and preparation of PLA-chitosan-PEG-glucose copolymer for combined delivery of Paclitaxel and siRNA |
| title_fullStr | Design and preparation of PLA-chitosan-PEG-glucose copolymer for combined delivery of Paclitaxel and siRNA |
| title_full_unstemmed | Design and preparation of PLA-chitosan-PEG-glucose copolymer for combined delivery of Paclitaxel and siRNA |
| title_short | Design and preparation of PLA-chitosan-PEG-glucose copolymer for combined delivery of Paclitaxel and siRNA |
| title_sort | design and preparation of pla chitosan peg glucose copolymer for combined delivery of paclitaxel and sirna |
| topic | Biocompatibility Cancer therapy Drug delivery Nanoparticle targeting PTX Transfection |
| url | https://doi.org/10.1007/s42452-025-07458-4 |
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