Determination of Benzalkonium Chloride in Nasal Drops by High-Performance Liquid Chromatography
A high-performance liquid chromatography (HPLC) system was used in the reversed phase mode for the determination of benzalkonium chloride (BKC) in nosal drops. A Chromolit RP-18e, 100 x 4.6, (UM6077/035) column was used at 40 °C. The mobile phase, optimized through an experimental design, was a 70:3...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/172070 |
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| author | Danijela A. Kostić Snezana S. Mitić Danijela Č. Nasković Aleksandra R. Zarubica Milan N. Mitic |
| author_facet | Danijela A. Kostić Snezana S. Mitić Danijela Č. Nasković Aleksandra R. Zarubica Milan N. Mitic |
| author_sort | Danijela A. Kostić |
| collection | DOAJ |
| description | A high-performance liquid chromatography (HPLC) system was used in the reversed phase mode for the determination of benzalkonium chloride (BKC) in nosal drops. A Chromolit RP-18e, 100 x 4.6, (UM6077/035) column was used at 40 °C. The mobile phase, optimized through an experimental design, was a 70:30 (v/v) mixture of 0.057M Na-heksansulphonate potassium, dihydrogen orthophosphate buffer (pH 2.9) and acetonitrile, pumped at a flow rate of 1.75 mL/min at maintaining column temperature at 40 °C. Maximum UV detection was achieved at 215 nm. The method was validated in terms of selectivity, linearity, repeatability, precision and accuracy. The method was successfully applied for the determination of BKC in a pharmaceutical formulation of nasal drop solution without any interference from common excipients and drug substance. All the validation parameters were within the acceptance range, concordant to ICH guidelines. |
| format | Article |
| id | doaj-art-c2b33ad29b4b4b0fb230881f2548ea53 |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-c2b33ad29b4b4b0fb230881f2548ea532025-02-03T07:25:50ZengWileyE-Journal of Chemistry0973-49452090-98102012-01-01931599160410.1155/2012/172070Determination of Benzalkonium Chloride in Nasal Drops by High-Performance Liquid ChromatographyDanijela A. Kostić0Snezana S. Mitić1Danijela Č. Nasković2Aleksandra R. Zarubica3Milan N. Mitic4Department of Chemistry, Faculty of Natural Sciences and Mathematics, Visegradska 33, 18000 Niš, SerbiaDepartment of Chemistry, Faculty of Natural Sciences and Mathematics, Visegradska 33, 18000 Niš, SerbiaD.D. “Zdravlje-Actavis”- Pharmaceutical and Chemical Company, 16000 Leskovac, SerbiaDepartment of Chemistry, Faculty of Natural Sciences and Mathematics, Visegradska 33, 18000 Niš, SerbiaDepartment of Chemistry, Faculty of Natural Sciences and Mathematics, Visegradska 33, 18000 Niš, SerbiaA high-performance liquid chromatography (HPLC) system was used in the reversed phase mode for the determination of benzalkonium chloride (BKC) in nosal drops. A Chromolit RP-18e, 100 x 4.6, (UM6077/035) column was used at 40 °C. The mobile phase, optimized through an experimental design, was a 70:30 (v/v) mixture of 0.057M Na-heksansulphonate potassium, dihydrogen orthophosphate buffer (pH 2.9) and acetonitrile, pumped at a flow rate of 1.75 mL/min at maintaining column temperature at 40 °C. Maximum UV detection was achieved at 215 nm. The method was validated in terms of selectivity, linearity, repeatability, precision and accuracy. The method was successfully applied for the determination of BKC in a pharmaceutical formulation of nasal drop solution without any interference from common excipients and drug substance. All the validation parameters were within the acceptance range, concordant to ICH guidelines.http://dx.doi.org/10.1155/2012/172070 |
| spellingShingle | Danijela A. Kostić Snezana S. Mitić Danijela Č. Nasković Aleksandra R. Zarubica Milan N. Mitic Determination of Benzalkonium Chloride in Nasal Drops by High-Performance Liquid Chromatography E-Journal of Chemistry |
| title | Determination of Benzalkonium Chloride in Nasal Drops by High-Performance Liquid Chromatography |
| title_full | Determination of Benzalkonium Chloride in Nasal Drops by High-Performance Liquid Chromatography |
| title_fullStr | Determination of Benzalkonium Chloride in Nasal Drops by High-Performance Liquid Chromatography |
| title_full_unstemmed | Determination of Benzalkonium Chloride in Nasal Drops by High-Performance Liquid Chromatography |
| title_short | Determination of Benzalkonium Chloride in Nasal Drops by High-Performance Liquid Chromatography |
| title_sort | determination of benzalkonium chloride in nasal drops by high performance liquid chromatography |
| url | http://dx.doi.org/10.1155/2012/172070 |
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