Continuous in situ synthesis of a complete set of tRNAs sustains steady-state translation in a recombinant cell-free system

Abstract Construction of a self-regenerating biochemical system is critical for building a synthetic cell. An essential step in building a self-regenerative system is producing a complete set of tRNAs for translation, which remains a significant challenge. Here, we reconstitute a complete set of 21...

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Bibliographic Details
Main Authors: Fanjun Li, Amogh Kumar Baranwal, Sebastian J. Maerkl
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-61671-8
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