Simultaneous determination of 11 synthetic colorants in wine by HPLC
Aiming to quickly identify whether synthetic colorants are added to wine and accurately quantify, a HPLC method was established to simultaneously detect 11 kinds of synthetic colorants in wine. The experimental samples were diluted with ammonium acetate solution, filtered directly after centrifugati...
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Editorial Department of China Brewing
2024-07-01
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| Series: | Zhongguo niangzao |
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| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-7-246.pdf |
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| author | LI Guang, LI Qi, GU Min, ZHANG Feng |
| author_facet | LI Guang, LI Qi, GU Min, ZHANG Feng |
| author_sort | LI Guang, LI Qi, GU Min, ZHANG Feng |
| collection | DOAJ |
| description | Aiming to quickly identify whether synthetic colorants are added to wine and accurately quantify, a HPLC method was established to simultaneously detect 11 kinds of synthetic colorants in wine. The experimental samples were diluted with ammonium acetate solution, filtered directly after centrifugation at 8 000 r/min, separated with Zorbax SB-C<sub>18</sub> column by gradient elution, and detected by multi-wavelength (288 nm, 425 nm, 515 nm, 620 nm). The results shown that the method was characterized by simple pre-treatment, no interference from impurities under the maximum absorption wavelength of each colorants, high sensitivity, low detection limit (0.05 mg/L), low limit of quantitation (0.15 mg/L), and good linear relationship (R<sup>2</sup>≥0.999 4) in the range of 0.20-50 mg/L. The relative standard deviation (RSD) of precision tests results was 1.86%-2.61%, and the recovery rates were 93.0%-103.0%. The method was suitable for rapid and accurate detection of synthetic colorants in wine. |
| format | Article |
| id | doaj-art-c2aec8b62e0b417cbf848d889ca5db73 |
| institution | DOAJ |
| issn | 0254-5071 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Editorial Department of China Brewing |
| record_format | Article |
| series | Zhongguo niangzao |
| spelling | doaj-art-c2aec8b62e0b417cbf848d889ca5db732025-08-20T02:55:02ZengEditorial Department of China BrewingZhongguo niangzao0254-50712024-07-0143724625010.11882/j.issn.0254-5071.2024.07.037Simultaneous determination of 11 synthetic colorants in wine by HPLCLI Guang, LI Qi, GU Min, ZHANG Feng0Analysis and Research Center of Gansu Province, Lanzhou 730000, ChinaAiming to quickly identify whether synthetic colorants are added to wine and accurately quantify, a HPLC method was established to simultaneously detect 11 kinds of synthetic colorants in wine. The experimental samples were diluted with ammonium acetate solution, filtered directly after centrifugation at 8 000 r/min, separated with Zorbax SB-C<sub>18</sub> column by gradient elution, and detected by multi-wavelength (288 nm, 425 nm, 515 nm, 620 nm). The results shown that the method was characterized by simple pre-treatment, no interference from impurities under the maximum absorption wavelength of each colorants, high sensitivity, low detection limit (0.05 mg/L), low limit of quantitation (0.15 mg/L), and good linear relationship (R<sup>2</sup>≥0.999 4) in the range of 0.20-50 mg/L. The relative standard deviation (RSD) of precision tests results was 1.86%-2.61%, and the recovery rates were 93.0%-103.0%. The method was suitable for rapid and accurate detection of synthetic colorants in wine.https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-7-246.pdfsynthetic colorants|wine|direct filtration|determination|hplc |
| spellingShingle | LI Guang, LI Qi, GU Min, ZHANG Feng Simultaneous determination of 11 synthetic colorants in wine by HPLC Zhongguo niangzao synthetic colorants|wine|direct filtration|determination|hplc |
| title | Simultaneous determination of 11 synthetic colorants in wine by HPLC |
| title_full | Simultaneous determination of 11 synthetic colorants in wine by HPLC |
| title_fullStr | Simultaneous determination of 11 synthetic colorants in wine by HPLC |
| title_full_unstemmed | Simultaneous determination of 11 synthetic colorants in wine by HPLC |
| title_short | Simultaneous determination of 11 synthetic colorants in wine by HPLC |
| title_sort | simultaneous determination of 11 synthetic colorants in wine by hplc |
| topic | synthetic colorants|wine|direct filtration|determination|hplc |
| url | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-7-246.pdf |
| work_keys_str_mv | AT liguangliqiguminzhangfeng simultaneousdeterminationof11syntheticcolorantsinwinebyhplc |