Simultaneous determination of 11 synthetic colorants in wine by HPLC
Aiming to quickly identify whether synthetic colorants are added to wine and accurately quantify, a HPLC method was established to simultaneously detect 11 kinds of synthetic colorants in wine. The experimental samples were diluted with ammonium acetate solution, filtered directly after centrifugati...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Editorial Department of China Brewing
2024-07-01
|
| Series: | Zhongguo niangzao |
| Subjects: | |
| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-7-246.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Aiming to quickly identify whether synthetic colorants are added to wine and accurately quantify, a HPLC method was established to simultaneously detect 11 kinds of synthetic colorants in wine. The experimental samples were diluted with ammonium acetate solution, filtered directly after centrifugation at 8 000 r/min, separated with Zorbax SB-C<sub>18</sub> column by gradient elution, and detected by multi-wavelength (288 nm, 425 nm, 515 nm, 620 nm). The results shown that the method was characterized by simple pre-treatment, no interference from impurities under the maximum absorption wavelength of each colorants, high sensitivity, low detection limit (0.05 mg/L), low limit of quantitation (0.15 mg/L), and good linear relationship (R<sup>2</sup>≥0.999 4) in the range of 0.20-50 mg/L. The relative standard deviation (RSD) of precision tests results was 1.86%-2.61%, and the recovery rates were 93.0%-103.0%. The method was suitable for rapid and accurate detection of synthetic colorants in wine. |
|---|---|
| ISSN: | 0254-5071 |