Retinal Vessel Diameter Reductions Are Associated with Retinal Ganglion Cell Dysfunction, Thinning of the Ganglion Cell and Inner Plexiform Layers, and Decreased Visual Field Global Indices in Glaucoma Suspects

<b>Background/Objectives</b>: The aim of this study was to evaluate the associations between optical coherence tomography angiography (OCTA)-based retinal vessel diameter (RVD) measurements, with retinal ganglion cell (RGC) function assessed by means of steady-state pattern electroretino...

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Main Authors: Andrew Tirsi, Nicholas Leung, Rohun Gupta, Sungmin Hong, Derek Orshan, Joby Tsai, Corey Ross Lacher, Isabella Tello, Samuel Potash, Timothy Foster, Rushil Kumbhani, Celso Tello
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Diagnostics
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Online Access:https://www.mdpi.com/2075-4418/15/13/1700
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author Andrew Tirsi
Nicholas Leung
Rohun Gupta
Sungmin Hong
Derek Orshan
Joby Tsai
Corey Ross Lacher
Isabella Tello
Samuel Potash
Timothy Foster
Rushil Kumbhani
Celso Tello
author_facet Andrew Tirsi
Nicholas Leung
Rohun Gupta
Sungmin Hong
Derek Orshan
Joby Tsai
Corey Ross Lacher
Isabella Tello
Samuel Potash
Timothy Foster
Rushil Kumbhani
Celso Tello
author_sort Andrew Tirsi
collection DOAJ
description <b>Background/Objectives</b>: The aim of this study was to evaluate the associations between optical coherence tomography angiography (OCTA)-based retinal vessel diameter (RVD) measurements, with retinal ganglion cell (RGC) function assessed by means of steady-state pattern electroretinography (ssPERG) using ganglion cell layer-inner plexiform layer thickness (GCL-IPLT) measurements and with Humphrey field analyzer (HFA) global indices in glaucoma suspects (GSs). <b>Methods</b>: Thirty-one eyes (20 participants) underwent a comprehensive ophthalmologic examination, ssPERG measurements utilizing the PERGLA paradigm, HFA, optical coherence tomography (OCT), and OCTA. The OCTA scans were processed using ImageJ software, Version 1.53, allowing for measurement of the RVD. Multiple linear regression models were used. <b>Results</b>: After controlling for age, race, central corneal thickness (CCT), and spherical equivalent (SE), a linear regression analysis found that the RVD explained the 4.7% variance in magnitude (Mag) (<i>p</i> = 0.169), 9.2% variance in magnitudeD (MagD) (<i>p</i> = 0.021), and 16.9% variance in magnitudeD/magnitude (<i>p</i> = 0.009). After controlling for age, CCT, and signal strength (SS), a linear regression analysis found that the RVD was significantly associated with the GCL-IPLT measurements (average GCL-IPL, minimum GCL-IPL, superior, superonasal, inferior, and inferonasal sectors) (<i>p</i> ≤ 0.023). An identical regression analysis where the RVD was replaced with the PERG parameters showed a significant association between the MagD and almost all GCI-IPLT measurements. RVD measurements were significantly associated with HFA VFI 24-2 (<i>p</i> = 0.004), MD 24-2 (<i>p</i> < 0.001), and PSD 24-2 (<i>p</i> = 0.009). <b>Conclusions</b>: Decreased RVD measurements were significantly associated with RGC dysfunction, decreased GCL-IPLT, and all HFA global indices in the GSs.
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spelling doaj-art-c2ab9dd1726f46c1bb7577c1dd681aca2025-08-20T03:50:17ZengMDPI AGDiagnostics2075-44182025-07-011513170010.3390/diagnostics15131700Retinal Vessel Diameter Reductions Are Associated with Retinal Ganglion Cell Dysfunction, Thinning of the Ganglion Cell and Inner Plexiform Layers, and Decreased Visual Field Global Indices in Glaucoma SuspectsAndrew Tirsi0Nicholas Leung1Rohun Gupta2Sungmin Hong3Derek Orshan4Joby Tsai5Corey Ross Lacher6Isabella Tello7Samuel Potash8Timothy Foster9Rushil Kumbhani10Celso Tello11Department of Ophthalmology, Manhattan Eye, Ear, and Throat Hospital, Northwell Health, New York, NY 10065, USADonald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY 11549, USADonald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY 11549, USADonald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY 11549, USADepartment of Ophthalmology, Larkin Community Hospital, Miami, FL 33431, USADepartment of Ophthalmology, Broward Health, Deerfield Beach, FL 33064, USADepartment of Ophthalmology, Rutgers New Jersey Medical School, Newark, NJ 07103, USADepartment of Ophthalmology, Manhattan Eye, Ear, and Throat Hospital, Northwell Health, New York, NY 10065, USADepartment of Ophthalmology, Rush University Medical Center, Chicago, IL 60612, USADonald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY 11549, USADonald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY 11549, USADepartment of Ophthalmology, Manhattan Eye, Ear, and Throat Hospital, Northwell Health, New York, NY 10065, USA<b>Background/Objectives</b>: The aim of this study was to evaluate the associations between optical coherence tomography angiography (OCTA)-based retinal vessel diameter (RVD) measurements, with retinal ganglion cell (RGC) function assessed by means of steady-state pattern electroretinography (ssPERG) using ganglion cell layer-inner plexiform layer thickness (GCL-IPLT) measurements and with Humphrey field analyzer (HFA) global indices in glaucoma suspects (GSs). <b>Methods</b>: Thirty-one eyes (20 participants) underwent a comprehensive ophthalmologic examination, ssPERG measurements utilizing the PERGLA paradigm, HFA, optical coherence tomography (OCT), and OCTA. The OCTA scans were processed using ImageJ software, Version 1.53, allowing for measurement of the RVD. Multiple linear regression models were used. <b>Results</b>: After controlling for age, race, central corneal thickness (CCT), and spherical equivalent (SE), a linear regression analysis found that the RVD explained the 4.7% variance in magnitude (Mag) (<i>p</i> = 0.169), 9.2% variance in magnitudeD (MagD) (<i>p</i> = 0.021), and 16.9% variance in magnitudeD/magnitude (<i>p</i> = 0.009). After controlling for age, CCT, and signal strength (SS), a linear regression analysis found that the RVD was significantly associated with the GCL-IPLT measurements (average GCL-IPL, minimum GCL-IPL, superior, superonasal, inferior, and inferonasal sectors) (<i>p</i> ≤ 0.023). An identical regression analysis where the RVD was replaced with the PERG parameters showed a significant association between the MagD and almost all GCI-IPLT measurements. RVD measurements were significantly associated with HFA VFI 24-2 (<i>p</i> = 0.004), MD 24-2 (<i>p</i> < 0.001), and PSD 24-2 (<i>p</i> = 0.009). <b>Conclusions</b>: Decreased RVD measurements were significantly associated with RGC dysfunction, decreased GCL-IPLT, and all HFA global indices in the GSs.https://www.mdpi.com/2075-4418/15/13/1700glaucomaretinal vessel diameterPERGretinal ganglion cellOCTAHumphrey field analyzer global indices
spellingShingle Andrew Tirsi
Nicholas Leung
Rohun Gupta
Sungmin Hong
Derek Orshan
Joby Tsai
Corey Ross Lacher
Isabella Tello
Samuel Potash
Timothy Foster
Rushil Kumbhani
Celso Tello
Retinal Vessel Diameter Reductions Are Associated with Retinal Ganglion Cell Dysfunction, Thinning of the Ganglion Cell and Inner Plexiform Layers, and Decreased Visual Field Global Indices in Glaucoma Suspects
Diagnostics
glaucoma
retinal vessel diameter
PERG
retinal ganglion cell
OCTA
Humphrey field analyzer global indices
title Retinal Vessel Diameter Reductions Are Associated with Retinal Ganglion Cell Dysfunction, Thinning of the Ganglion Cell and Inner Plexiform Layers, and Decreased Visual Field Global Indices in Glaucoma Suspects
title_full Retinal Vessel Diameter Reductions Are Associated with Retinal Ganglion Cell Dysfunction, Thinning of the Ganglion Cell and Inner Plexiform Layers, and Decreased Visual Field Global Indices in Glaucoma Suspects
title_fullStr Retinal Vessel Diameter Reductions Are Associated with Retinal Ganglion Cell Dysfunction, Thinning of the Ganglion Cell and Inner Plexiform Layers, and Decreased Visual Field Global Indices in Glaucoma Suspects
title_full_unstemmed Retinal Vessel Diameter Reductions Are Associated with Retinal Ganglion Cell Dysfunction, Thinning of the Ganglion Cell and Inner Plexiform Layers, and Decreased Visual Field Global Indices in Glaucoma Suspects
title_short Retinal Vessel Diameter Reductions Are Associated with Retinal Ganglion Cell Dysfunction, Thinning of the Ganglion Cell and Inner Plexiform Layers, and Decreased Visual Field Global Indices in Glaucoma Suspects
title_sort retinal vessel diameter reductions are associated with retinal ganglion cell dysfunction thinning of the ganglion cell and inner plexiform layers and decreased visual field global indices in glaucoma suspects
topic glaucoma
retinal vessel diameter
PERG
retinal ganglion cell
OCTA
Humphrey field analyzer global indices
url https://www.mdpi.com/2075-4418/15/13/1700
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