Immune Responses Induced by Recombinant Membrane Proteins of <i>Mycoplasma agalactiae</i> in Goats
<b>Background/Objectives</b>: Contagious agalactia (CA) is a disease typically caused by Mycoplasma agalactiae, affecting small ruminants worldwide and being endemic in certain countries. CA causes severe economic losses due to mastitis, agalactia, and arthritis. As an alternative to exi...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
|
| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/13/7/746 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849713725779476480 |
|---|---|
| author | Beatriz Almeida Sampaio Maysa Santos Barbosa Matheus Gonçalves de Oliveira Manoel Neres Santos Júnior Bruna Carolina de Brito Guimarães Emilly Stefane Souza Andres Ágatha Morgana Bertoti da Silva Camila Pacheco Gomes Rafaela de Souza Bittencourt Thiago Macêdo Lopes Correia Lucas Santana Coelho da Silva Jurandir Ferreira da Cruz Rohini Chopra-Dewasthaly Guilherme Barreto Campos Jorge Timenetsky Bruno Lopes Bastos Lucas Miranda Marques |
| author_facet | Beatriz Almeida Sampaio Maysa Santos Barbosa Matheus Gonçalves de Oliveira Manoel Neres Santos Júnior Bruna Carolina de Brito Guimarães Emilly Stefane Souza Andres Ágatha Morgana Bertoti da Silva Camila Pacheco Gomes Rafaela de Souza Bittencourt Thiago Macêdo Lopes Correia Lucas Santana Coelho da Silva Jurandir Ferreira da Cruz Rohini Chopra-Dewasthaly Guilherme Barreto Campos Jorge Timenetsky Bruno Lopes Bastos Lucas Miranda Marques |
| author_sort | Beatriz Almeida Sampaio |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: Contagious agalactia (CA) is a disease typically caused by Mycoplasma agalactiae, affecting small ruminants worldwide and being endemic in certain countries. CA causes severe economic losses due to mastitis, agalactia, and arthritis. As an alternative to existing immunoprophylactic measures, this study aimed to develop a recombinant subunit vaccine against <i>M. agalactiae</i> and evaluate its specific immune response in goats. <b>Methods</b>: Goats were divided into three groups: group 1 received recombinant proteins (P40 and MAG_1560), group 2 received formalin-inactivated <i>M. agalactiae</i>, and group 3 received Tris-buffered saline (negative control). All solutions were emulsified in Freund’s adjuvant. Animals were monitored for 181 days. IgG antibody production was assessed by ELISA, and peripheral blood mononuclear cells (PBMCs) were analyzed by real-time PCR for the expression of IL-1β, IFN-γ, IL-12, and MHC class II genes. <b>Results</b>: <i>M. agalactiae</i>-specific antibody response was observed for six months in the sera of animals from group 1. Analysis of cytokine gene expression revealed increased IL-1β mRNA levels over time in both experimental groups. In group 1, IFN-γ mRNA levels increased with P40 stimulation and decreased with MAG_1560. IL-12 mRNA expression decreased over time in group 1 with P40 stimulation, whereas group 2 showed increased IL-12 expression for both proteins. MHC-II expression was stimulated in both groups. <b>Conclusions</b>: The recombinant proteins induced antibody production and cytokine expression, demonstrating immunogenic potential and supporting their promise as vaccine candidates capable of eliciting both humoral and cellular immune responses against <i>M. agalactiae</i>. |
| format | Article |
| id | doaj-art-c2a33f9161ce4de09948017651bcb525 |
| institution | DOAJ |
| issn | 2076-393X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj-art-c2a33f9161ce4de09948017651bcb5252025-08-20T03:13:54ZengMDPI AGVaccines2076-393X2025-07-0113774610.3390/vaccines13070746Immune Responses Induced by Recombinant Membrane Proteins of <i>Mycoplasma agalactiae</i> in GoatsBeatriz Almeida Sampaio0Maysa Santos Barbosa1Matheus Gonçalves de Oliveira2Manoel Neres Santos Júnior3Bruna Carolina de Brito Guimarães4Emilly Stefane Souza Andres5Ágatha Morgana Bertoti da Silva6Camila Pacheco Gomes7Rafaela de Souza Bittencourt8Thiago Macêdo Lopes Correia9Lucas Santana Coelho da Silva10Jurandir Ferreira da Cruz11Rohini Chopra-Dewasthaly12Guilherme Barreto Campos13Jorge Timenetsky14Bruno Lopes Bastos15Lucas Miranda Marques16Multidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, BrazilInstitute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes, 2415, Butantã 05508-900, SP, BrazilMultidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, BrazilMicrobiology Department, State University of Santa Cruz, Rod. Jorge Amado, Km a6, Ilhéus 45662-900, BA, BrazilMicrobiology Department, State University of Santa Cruz, Rod. Jorge Amado, Km a6, Ilhéus 45662-900, BA, BrazilMultidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, BrazilMultidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, BrazilMicrobiology Department, State University of Santa Cruz, Rod. Jorge Amado, Km a6, Ilhéus 45662-900, BA, BrazilMultidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, BrazilMultidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, BrazilMicrobiology Department, State University of Santa Cruz, Rod. Jorge Amado, Km a6, Ilhéus 45662-900, BA, BrazilGoat Sheep Sector, Department of Plant Science and Animal Science, State University of Southwest Bahia, Estrada do Bem Querer, Km 4, Vitória da Conquista 45083-900, BA, BrazilDepartment of Pathobiology, Institute of Microbiology, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, AustriaMultidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, BrazilInstitute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes, 2415, Butantã 05508-900, SP, BrazilMultidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, BrazilMultidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, Brazil<b>Background/Objectives</b>: Contagious agalactia (CA) is a disease typically caused by Mycoplasma agalactiae, affecting small ruminants worldwide and being endemic in certain countries. CA causes severe economic losses due to mastitis, agalactia, and arthritis. As an alternative to existing immunoprophylactic measures, this study aimed to develop a recombinant subunit vaccine against <i>M. agalactiae</i> and evaluate its specific immune response in goats. <b>Methods</b>: Goats were divided into three groups: group 1 received recombinant proteins (P40 and MAG_1560), group 2 received formalin-inactivated <i>M. agalactiae</i>, and group 3 received Tris-buffered saline (negative control). All solutions were emulsified in Freund’s adjuvant. Animals were monitored for 181 days. IgG antibody production was assessed by ELISA, and peripheral blood mononuclear cells (PBMCs) were analyzed by real-time PCR for the expression of IL-1β, IFN-γ, IL-12, and MHC class II genes. <b>Results</b>: <i>M. agalactiae</i>-specific antibody response was observed for six months in the sera of animals from group 1. Analysis of cytokine gene expression revealed increased IL-1β mRNA levels over time in both experimental groups. In group 1, IFN-γ mRNA levels increased with P40 stimulation and decreased with MAG_1560. IL-12 mRNA expression decreased over time in group 1 with P40 stimulation, whereas group 2 showed increased IL-12 expression for both proteins. MHC-II expression was stimulated in both groups. <b>Conclusions</b>: The recombinant proteins induced antibody production and cytokine expression, demonstrating immunogenic potential and supporting their promise as vaccine candidates capable of eliciting both humoral and cellular immune responses against <i>M. agalactiae</i>.https://www.mdpi.com/2076-393X/13/7/746contagious agalactia<i>M. agalactiae</i>recombinantsubunitvaccine |
| spellingShingle | Beatriz Almeida Sampaio Maysa Santos Barbosa Matheus Gonçalves de Oliveira Manoel Neres Santos Júnior Bruna Carolina de Brito Guimarães Emilly Stefane Souza Andres Ágatha Morgana Bertoti da Silva Camila Pacheco Gomes Rafaela de Souza Bittencourt Thiago Macêdo Lopes Correia Lucas Santana Coelho da Silva Jurandir Ferreira da Cruz Rohini Chopra-Dewasthaly Guilherme Barreto Campos Jorge Timenetsky Bruno Lopes Bastos Lucas Miranda Marques Immune Responses Induced by Recombinant Membrane Proteins of <i>Mycoplasma agalactiae</i> in Goats Vaccines contagious agalactia <i>M. agalactiae</i> recombinant subunit vaccine |
| title | Immune Responses Induced by Recombinant Membrane Proteins of <i>Mycoplasma agalactiae</i> in Goats |
| title_full | Immune Responses Induced by Recombinant Membrane Proteins of <i>Mycoplasma agalactiae</i> in Goats |
| title_fullStr | Immune Responses Induced by Recombinant Membrane Proteins of <i>Mycoplasma agalactiae</i> in Goats |
| title_full_unstemmed | Immune Responses Induced by Recombinant Membrane Proteins of <i>Mycoplasma agalactiae</i> in Goats |
| title_short | Immune Responses Induced by Recombinant Membrane Proteins of <i>Mycoplasma agalactiae</i> in Goats |
| title_sort | immune responses induced by recombinant membrane proteins of i mycoplasma agalactiae i in goats |
| topic | contagious agalactia <i>M. agalactiae</i> recombinant subunit vaccine |
| url | https://www.mdpi.com/2076-393X/13/7/746 |
| work_keys_str_mv | AT beatrizalmeidasampaio immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats AT maysasantosbarbosa immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats AT matheusgoncalvesdeoliveira immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats AT manoelneressantosjunior immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats AT brunacarolinadebritoguimaraes immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats AT emillystefanesouzaandres immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats AT agathamorganabertotidasilva immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats AT camilapachecogomes immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats AT rafaeladesouzabittencourt immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats AT thiagomacedolopescorreia immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats AT lucassantanacoelhodasilva immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats AT jurandirferreiradacruz immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats AT rohinichopradewasthaly immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats AT guilhermebarretocampos immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats AT jorgetimenetsky immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats AT brunolopesbastos immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats AT lucasmirandamarques immuneresponsesinducedbyrecombinantmembraneproteinsofimycoplasmaagalactiaeiingoats |