Effects of Fish Oil Replacement With Degossypolized Cottonseed Oil on Growth Performance, Proximate Composition, Lipid Metabolism, and Liver Function of Large Yellow Croaker (Larimichthys crocea) Juvenile
A 10-week feeding trial was conducted with juvenile large yellow croaker, Larimichthys crocea, to evaluate the effects of degossypolized cottonseed oil (DCSO) as a potential replacement for fish oil (FO). Five isonitrogenous (42% crude protein) and isolipidic (13% crude fat) diets were formulated wh...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Aquaculture Nutrition |
| Online Access: | http://dx.doi.org/10.1155/anu/4241105 |
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| author | Md. Golam Sajed Riar Nur A Raushon Shijie Pan Jinze Zhang Chukwuma Kenneth Chibuikem Yueru Li Kangsen Mai Qinghui Ai |
| author_facet | Md. Golam Sajed Riar Nur A Raushon Shijie Pan Jinze Zhang Chukwuma Kenneth Chibuikem Yueru Li Kangsen Mai Qinghui Ai |
| author_sort | Md. Golam Sajed Riar |
| collection | DOAJ |
| description | A 10-week feeding trial was conducted with juvenile large yellow croaker, Larimichthys crocea, to evaluate the effects of degossypolized cottonseed oil (DCSO) as a potential replacement for fish oil (FO). Five isonitrogenous (42% crude protein) and isolipidic (13% crude fat) diets were formulated where DCSO replaced FO at 0%, 25%, 50%, 75%, and 100% (DCSO0, DCSO25, DCSO50, DCSO75, and DCSO100) to determine their effects on growth performance, feed utilization and tissue lipid compositions of experimental fish. The initial body weight of the experimental fish was 8.28 ± 0.99 g. Results showed that substituting up to 50% of FO with DCSO does not significantly affect the fish’s survival rate (SR), growth performance, or proximate composition. However, exceeding a 50% replacement significantly decreases (p<0.05) growth performance and feed efficiency. Additionally, significant increases (p<0.05) in viscerosomatic index (VSI) and hepatosomatic index (HSI) are observed at higher DCSO inclusion levels. Fatty acid analysis shows that DCSO is rich in n-6 polyunsaturated fatty acids (PUFA). Still, low in n-3 PUFA alters fatty acid profiles in the liver and muscle, resulting in elevated total cholesterol (T-CHO), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) levels, alongside increased activities of liver enzymes, indicating compromised liver function. While antioxidant enzyme levels remain stable, malondialdehyde (MDA) levels rise, suggesting elevated oxidative stress. Furthermore, lipase activity declines significantly (p<0.05) at 50% FO replacement, with upregulated lipid synthesis genes (srebp1, fatp1, fas, and ppar-α) leading to higher lipid accumulation. Inflammatory response gene expression is also significantly affected, showing amplified pro-inflammatory (ifnγ, il-1β, and tnfα) and decreased anti-inflammatory (il-10, arg-1, tgfβ) cytokines at higher DCSO levels. In conclusion, while up to 50% replacement of FO with DCSO is acceptable, surpassing this threshold adversely affects growth, liver function, lipid metabolism, and inflammatory responses in juvenile large yellow croaker. |
| format | Article |
| id | doaj-art-c2a1a64329404ca88a0eb36878d83b79 |
| institution | OA Journals |
| issn | 1365-2095 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aquaculture Nutrition |
| spelling | doaj-art-c2a1a64329404ca88a0eb36878d83b792025-08-20T02:37:17ZengWileyAquaculture Nutrition1365-20952025-01-01202510.1155/anu/4241105Effects of Fish Oil Replacement With Degossypolized Cottonseed Oil on Growth Performance, Proximate Composition, Lipid Metabolism, and Liver Function of Large Yellow Croaker (Larimichthys crocea) JuvenileMd. Golam Sajed Riar0Nur A Raushon1Shijie Pan2Jinze Zhang3Chukwuma Kenneth Chibuikem4Yueru Li5Kangsen Mai6Qinghui Ai7Key laboratory of Aquaculture Nutrition and FeedKey laboratory of Aquaculture Nutrition and FeedKey laboratory of Aquaculture Nutrition and FeedKey laboratory of Aquaculture Nutrition and FeedKey laboratory of Aquaculture Nutrition and FeedKey laboratory of Aquaculture Nutrition and FeedKey laboratory of Aquaculture Nutrition and FeedKey laboratory of Aquaculture Nutrition and FeedA 10-week feeding trial was conducted with juvenile large yellow croaker, Larimichthys crocea, to evaluate the effects of degossypolized cottonseed oil (DCSO) as a potential replacement for fish oil (FO). Five isonitrogenous (42% crude protein) and isolipidic (13% crude fat) diets were formulated where DCSO replaced FO at 0%, 25%, 50%, 75%, and 100% (DCSO0, DCSO25, DCSO50, DCSO75, and DCSO100) to determine their effects on growth performance, feed utilization and tissue lipid compositions of experimental fish. The initial body weight of the experimental fish was 8.28 ± 0.99 g. Results showed that substituting up to 50% of FO with DCSO does not significantly affect the fish’s survival rate (SR), growth performance, or proximate composition. However, exceeding a 50% replacement significantly decreases (p<0.05) growth performance and feed efficiency. Additionally, significant increases (p<0.05) in viscerosomatic index (VSI) and hepatosomatic index (HSI) are observed at higher DCSO inclusion levels. Fatty acid analysis shows that DCSO is rich in n-6 polyunsaturated fatty acids (PUFA). Still, low in n-3 PUFA alters fatty acid profiles in the liver and muscle, resulting in elevated total cholesterol (T-CHO), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) levels, alongside increased activities of liver enzymes, indicating compromised liver function. While antioxidant enzyme levels remain stable, malondialdehyde (MDA) levels rise, suggesting elevated oxidative stress. Furthermore, lipase activity declines significantly (p<0.05) at 50% FO replacement, with upregulated lipid synthesis genes (srebp1, fatp1, fas, and ppar-α) leading to higher lipid accumulation. Inflammatory response gene expression is also significantly affected, showing amplified pro-inflammatory (ifnγ, il-1β, and tnfα) and decreased anti-inflammatory (il-10, arg-1, tgfβ) cytokines at higher DCSO levels. In conclusion, while up to 50% replacement of FO with DCSO is acceptable, surpassing this threshold adversely affects growth, liver function, lipid metabolism, and inflammatory responses in juvenile large yellow croaker.http://dx.doi.org/10.1155/anu/4241105 |
| spellingShingle | Md. Golam Sajed Riar Nur A Raushon Shijie Pan Jinze Zhang Chukwuma Kenneth Chibuikem Yueru Li Kangsen Mai Qinghui Ai Effects of Fish Oil Replacement With Degossypolized Cottonseed Oil on Growth Performance, Proximate Composition, Lipid Metabolism, and Liver Function of Large Yellow Croaker (Larimichthys crocea) Juvenile Aquaculture Nutrition |
| title | Effects of Fish Oil Replacement With Degossypolized Cottonseed Oil on Growth Performance, Proximate Composition, Lipid Metabolism, and Liver Function of Large Yellow Croaker (Larimichthys crocea) Juvenile |
| title_full | Effects of Fish Oil Replacement With Degossypolized Cottonseed Oil on Growth Performance, Proximate Composition, Lipid Metabolism, and Liver Function of Large Yellow Croaker (Larimichthys crocea) Juvenile |
| title_fullStr | Effects of Fish Oil Replacement With Degossypolized Cottonseed Oil on Growth Performance, Proximate Composition, Lipid Metabolism, and Liver Function of Large Yellow Croaker (Larimichthys crocea) Juvenile |
| title_full_unstemmed | Effects of Fish Oil Replacement With Degossypolized Cottonseed Oil on Growth Performance, Proximate Composition, Lipid Metabolism, and Liver Function of Large Yellow Croaker (Larimichthys crocea) Juvenile |
| title_short | Effects of Fish Oil Replacement With Degossypolized Cottonseed Oil on Growth Performance, Proximate Composition, Lipid Metabolism, and Liver Function of Large Yellow Croaker (Larimichthys crocea) Juvenile |
| title_sort | effects of fish oil replacement with degossypolized cottonseed oil on growth performance proximate composition lipid metabolism and liver function of large yellow croaker larimichthys crocea juvenile |
| url | http://dx.doi.org/10.1155/anu/4241105 |
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