Development of nano hydroxyapatite loaded gellan gum nanocomposite scaffold for the regeneration of bone tissue affected by osteosarcoma
Advanced biomaterials play a critical role in promoting bone tissue engineering and regenerative medicine applications. This study aimed to fabricate a biomimetic scaffold incorporating nano-hydroxyapatite (nHA) and gellan gum (GG) for bone tissue repair. Nanocomposite scaffold loaded with nHA nanop...
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Elsevier
2025-05-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715625001912 |
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| author | Huaidong Wang Tao Bai Mahani Yusoff Nur Ain Atisya C.M. Khairuddin Alina Irwana Muhamad A'srai Mohd Hasmizam Razali |
| author_facet | Huaidong Wang Tao Bai Mahani Yusoff Nur Ain Atisya C.M. Khairuddin Alina Irwana Muhamad A'srai Mohd Hasmizam Razali |
| author_sort | Huaidong Wang |
| collection | DOAJ |
| description | Advanced biomaterials play a critical role in promoting bone tissue engineering and regenerative medicine applications. This study aimed to fabricate a biomimetic scaffold incorporating nano-hydroxyapatite (nHA) and gellan gum (GG) for bone tissue repair. Nanocomposite scaffold loaded with nHA nanoparticles within a gellan gum matrix (nHA@GG) was successfully produced using a freeze-drying technique. Characterization by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), and Scanning Electron Microscopy (SEM) corroborated the successful fabrication of the nHA@GG nanocomposite scaffold. XRD and FTIR analyses verified the phase composition and chemical bonding characteristics of hydroxyapatite, aligning with its successful formation within the scaffold. These findings highlight the potential of the nHA@GG nanocomposite scaffold to facilitate and accelerate apatite growth in in-vitro models. This might be attributed to the presence of nHA, which can act as a nucleation site for secondary apatite layer formation. The nHA@GG scaffold demonstrated efficient encapsulation and drug loading, with 35.48 ± 0.84 % encapsulation and 25.75 ± 0.75 % drug loading of sodium alendronate. The cumulative drug release of 38 % was achieved within 4 days. Furthermore, biocompatibility studies utilizing MC3T3 cells demonstrated significant cell proliferation (>100 %) and high cell viability (>90 %) on the nHA@GG scaffold. These findings, coupled with the favorable physicochemical properties and non-toxic nature of the nHA@GG scaffold, highlight its promising potential as a biomaterial for bone tissue regeneration. |
| format | Article |
| id | doaj-art-c29f2db2b1b0473baaae8a36502226a5 |
| institution | OA Journals |
| issn | 2211-7156 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj-art-c29f2db2b1b0473baaae8a36502226a52025-08-20T02:35:44ZengElsevierResults in Chemistry2211-71562025-05-011510220810.1016/j.rechem.2025.102208Development of nano hydroxyapatite loaded gellan gum nanocomposite scaffold for the regeneration of bone tissue affected by osteosarcomaHuaidong Wang0Tao Bai1Mahani Yusoff2Nur Ain Atisya C.M. Khairuddin3Alina Irwana Muhamad A'srai4Mohd Hasmizam Razali5Department of Orthopaedics, XD Group Hospital, Xi'an 710077, ChinaDepartment of Orthopedics, Yan'an People's Hospital, Yan'an 716000, China; Corresponding author.Faculty of Bioengineering and Technology, Universiti Malaysia Kelantan, 17600 Jeli, Kelantan, MalaysiaFaculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, MalaysiaFaculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, MalaysiaFaculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia; Advanced Nanomaterials Research Group, Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia; Corresponding author at: Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia.Advanced biomaterials play a critical role in promoting bone tissue engineering and regenerative medicine applications. This study aimed to fabricate a biomimetic scaffold incorporating nano-hydroxyapatite (nHA) and gellan gum (GG) for bone tissue repair. Nanocomposite scaffold loaded with nHA nanoparticles within a gellan gum matrix (nHA@GG) was successfully produced using a freeze-drying technique. Characterization by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), and Scanning Electron Microscopy (SEM) corroborated the successful fabrication of the nHA@GG nanocomposite scaffold. XRD and FTIR analyses verified the phase composition and chemical bonding characteristics of hydroxyapatite, aligning with its successful formation within the scaffold. These findings highlight the potential of the nHA@GG nanocomposite scaffold to facilitate and accelerate apatite growth in in-vitro models. This might be attributed to the presence of nHA, which can act as a nucleation site for secondary apatite layer formation. The nHA@GG scaffold demonstrated efficient encapsulation and drug loading, with 35.48 ± 0.84 % encapsulation and 25.75 ± 0.75 % drug loading of sodium alendronate. The cumulative drug release of 38 % was achieved within 4 days. Furthermore, biocompatibility studies utilizing MC3T3 cells demonstrated significant cell proliferation (>100 %) and high cell viability (>90 %) on the nHA@GG scaffold. These findings, coupled with the favorable physicochemical properties and non-toxic nature of the nHA@GG scaffold, highlight its promising potential as a biomaterial for bone tissue regeneration.http://www.sciencedirect.com/science/article/pii/S2211715625001912HydroxyapatiteNanocompositeScaffoldBone tissueOsteosarcoma |
| spellingShingle | Huaidong Wang Tao Bai Mahani Yusoff Nur Ain Atisya C.M. Khairuddin Alina Irwana Muhamad A'srai Mohd Hasmizam Razali Development of nano hydroxyapatite loaded gellan gum nanocomposite scaffold for the regeneration of bone tissue affected by osteosarcoma Results in Chemistry Hydroxyapatite Nanocomposite Scaffold Bone tissue Osteosarcoma |
| title | Development of nano hydroxyapatite loaded gellan gum nanocomposite scaffold for the regeneration of bone tissue affected by osteosarcoma |
| title_full | Development of nano hydroxyapatite loaded gellan gum nanocomposite scaffold for the regeneration of bone tissue affected by osteosarcoma |
| title_fullStr | Development of nano hydroxyapatite loaded gellan gum nanocomposite scaffold for the regeneration of bone tissue affected by osteosarcoma |
| title_full_unstemmed | Development of nano hydroxyapatite loaded gellan gum nanocomposite scaffold for the regeneration of bone tissue affected by osteosarcoma |
| title_short | Development of nano hydroxyapatite loaded gellan gum nanocomposite scaffold for the regeneration of bone tissue affected by osteosarcoma |
| title_sort | development of nano hydroxyapatite loaded gellan gum nanocomposite scaffold for the regeneration of bone tissue affected by osteosarcoma |
| topic | Hydroxyapatite Nanocomposite Scaffold Bone tissue Osteosarcoma |
| url | http://www.sciencedirect.com/science/article/pii/S2211715625001912 |
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