Frontiers in nanoparticles redefining enzyme immobilization: a review addressing challenges, innovations, and unlocking sustainable future potentials
Abstract Nanoparticles (NPs) are redefining enzyme immobilization, offering a paradigm shift in biocatalysis through precision engineering at the nanoscale. With their exceptional surface area, tunable porosity, and customizable functionalities, NPs provide unprecedented control over enzyme stabilit...
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SpringerOpen
2025-05-01
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| Series: | Micro and Nano Systems Letters |
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| Online Access: | https://doi.org/10.1186/s40486-025-00228-2 |
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| author | Heidi M. Abdel-Mageed |
| author_facet | Heidi M. Abdel-Mageed |
| author_sort | Heidi M. Abdel-Mageed |
| collection | DOAJ |
| description | Abstract Nanoparticles (NPs) are redefining enzyme immobilization, offering a paradigm shift in biocatalysis through precision engineering at the nanoscale. With their exceptional surface area, tunable porosity, and customizable functionalities, NPs provide unprecedented control over enzyme stability, activity, and adaptability, bridging the gap between molecular-scale interactions and industrial-scale applications. In the era of intelligent bioprocessing, how can NP-based immobilization strategies be optimized to drive the next frontier of sustainable and high-performance enzyme technologies? A deep understanding of NP structural diversity, interfacial chemistry, and enzyme-matrix interactions is crucial to unlocking their full potential. This review systematically explores emerging NP-based immobilization platforms, including cross-linked enzyme aggregates (CLEAs), covalent organic frameworks (COFs), nanoflowers, nanofibers, carbon nanotubes (CNTs), graphene oxide (GO), ionic liquids (ILs), and layered double hydroxides (LDHs), each offering tailored advantages for catalytic enhancement and process efficiency. The review outlines current advancements such as 3D printing and wearable biosensors, forecasts the integration of artificial intelligence and smart nano-biocatalysts, and envisions futuristic applications including bio-intelligent nano/micro-robotic systems and space biosensors. Challenges, such as upscaling limitations, nanotoxicity concerns, and environmental risks, are addressed to ensure safe and viable implementation. This review provides a structured roadmap on (I) enzyme immobilization advances using next-generation NPs, (II) challenges in scalability and safety, (III) sustainability benefits of enzyme-based industrial biocatalysis, and (IV) the emergence of intelligence, adaptability, and nanoscale precision immobilization technologies and AI-assisted design and optimization. These visionary approaches mark a paradigm shift toward dynamic, adaptive, and highly specialized, multifunctional nano-enzyme systems. Graphical Abstract |
| format | Article |
| id | doaj-art-c2980866bd9b4e3ebe7a5db18c6ddc60 |
| institution | DOAJ |
| issn | 2213-9621 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Micro and Nano Systems Letters |
| spelling | doaj-art-c2980866bd9b4e3ebe7a5db18c6ddc602025-08-20T03:10:30ZengSpringerOpenMicro and Nano Systems Letters2213-96212025-05-0113112810.1186/s40486-025-00228-2Frontiers in nanoparticles redefining enzyme immobilization: a review addressing challenges, innovations, and unlocking sustainable future potentialsHeidi M. Abdel-Mageed0Molecular Biology Department, National Research CentreAbstract Nanoparticles (NPs) are redefining enzyme immobilization, offering a paradigm shift in biocatalysis through precision engineering at the nanoscale. With their exceptional surface area, tunable porosity, and customizable functionalities, NPs provide unprecedented control over enzyme stability, activity, and adaptability, bridging the gap between molecular-scale interactions and industrial-scale applications. In the era of intelligent bioprocessing, how can NP-based immobilization strategies be optimized to drive the next frontier of sustainable and high-performance enzyme technologies? A deep understanding of NP structural diversity, interfacial chemistry, and enzyme-matrix interactions is crucial to unlocking their full potential. This review systematically explores emerging NP-based immobilization platforms, including cross-linked enzyme aggregates (CLEAs), covalent organic frameworks (COFs), nanoflowers, nanofibers, carbon nanotubes (CNTs), graphene oxide (GO), ionic liquids (ILs), and layered double hydroxides (LDHs), each offering tailored advantages for catalytic enhancement and process efficiency. The review outlines current advancements such as 3D printing and wearable biosensors, forecasts the integration of artificial intelligence and smart nano-biocatalysts, and envisions futuristic applications including bio-intelligent nano/micro-robotic systems and space biosensors. Challenges, such as upscaling limitations, nanotoxicity concerns, and environmental risks, are addressed to ensure safe and viable implementation. This review provides a structured roadmap on (I) enzyme immobilization advances using next-generation NPs, (II) challenges in scalability and safety, (III) sustainability benefits of enzyme-based industrial biocatalysis, and (IV) the emergence of intelligence, adaptability, and nanoscale precision immobilization technologies and AI-assisted design and optimization. These visionary approaches mark a paradigm shift toward dynamic, adaptive, and highly specialized, multifunctional nano-enzyme systems. Graphical Abstracthttps://doi.org/10.1186/s40486-025-00228-2Nanoparticles nanotechnologyEnzyme immobilizationNanoflowers and nanofibersSmart immobilizationStabilityArtificial intelligence (AI) |
| spellingShingle | Heidi M. Abdel-Mageed Frontiers in nanoparticles redefining enzyme immobilization: a review addressing challenges, innovations, and unlocking sustainable future potentials Micro and Nano Systems Letters Nanoparticles nanotechnology Enzyme immobilization Nanoflowers and nanofibers Smart immobilization Stability Artificial intelligence (AI) |
| title | Frontiers in nanoparticles redefining enzyme immobilization: a review addressing challenges, innovations, and unlocking sustainable future potentials |
| title_full | Frontiers in nanoparticles redefining enzyme immobilization: a review addressing challenges, innovations, and unlocking sustainable future potentials |
| title_fullStr | Frontiers in nanoparticles redefining enzyme immobilization: a review addressing challenges, innovations, and unlocking sustainable future potentials |
| title_full_unstemmed | Frontiers in nanoparticles redefining enzyme immobilization: a review addressing challenges, innovations, and unlocking sustainable future potentials |
| title_short | Frontiers in nanoparticles redefining enzyme immobilization: a review addressing challenges, innovations, and unlocking sustainable future potentials |
| title_sort | frontiers in nanoparticles redefining enzyme immobilization a review addressing challenges innovations and unlocking sustainable future potentials |
| topic | Nanoparticles nanotechnology Enzyme immobilization Nanoflowers and nanofibers Smart immobilization Stability Artificial intelligence (AI) |
| url | https://doi.org/10.1186/s40486-025-00228-2 |
| work_keys_str_mv | AT heidimabdelmageed frontiersinnanoparticlesredefiningenzymeimmobilizationareviewaddressingchallengesinnovationsandunlockingsustainablefuturepotentials |