Effect of Topically Administered Chitosan-N-acetylcysteine on Corneal Wound Healing in a Rabbit Model
Purpose. The present study was performed to investigate the effect of topically administered chitosan-N-acetylcysteine (C-NAC) on corneal wound healing in a rabbit model. Methods. A total of 20 New Zealand White rabbits were included in the randomized, masked, placebo-controlled experiment. A monocu...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2017/5192924 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554175972507648 |
|---|---|
| author | Corinna Fischak Robert Klaus René M. Werkmeister Christine Hohenadl Martin Prinz Leopold Schmetterer Gerhard Garhöfer |
| author_facet | Corinna Fischak Robert Klaus René M. Werkmeister Christine Hohenadl Martin Prinz Leopold Schmetterer Gerhard Garhöfer |
| author_sort | Corinna Fischak |
| collection | DOAJ |
| description | Purpose. The present study was performed to investigate the effect of topically administered chitosan-N-acetylcysteine (C-NAC) on corneal wound healing in a rabbit model. Methods. A total of 20 New Zealand White rabbits were included in the randomized, masked, placebo-controlled experiment. A monocular epithelial debridement was induced by manual scraping under general anesthesia. Animals were randomized to receive either C-NAC two times daily or placebo. Monitoring of corneal wound healing was performed with ultra-high-resolution optical coherence tomography (OCT) and epithelial fluorescein staining. Measurements were done immediately after and up to 72 hours after wound induction. Results. No difference in wound size was found immediately after surgical debridement between the C-NAC group and the placebo group. Wound healing was significantly faster in the C-NAC group compared to the placebo group (p<0.01 for both methods). A good correlation was found between the OCT technique and the epithelial fluorescein staining in terms of wound size (r=0.94). Conclusions. Administration of C-NAC containing eye drops twice daily leads to a faster corneal wound healing in a rabbit model of corneal debridement as compared to placebo. Ultra-high-resolution OCT is considered a noninvasive, dye-free alternative to conventional fluorescein staining in assessing corneal wound healing also in humans. |
| format | Article |
| id | doaj-art-c293f80183cf45bd8fab99eddec9c475 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-c293f80183cf45bd8fab99eddec9c4752025-02-03T05:52:13ZengWileyJournal of Ophthalmology2090-004X2090-00582017-01-01201710.1155/2017/51929245192924Effect of Topically Administered Chitosan-N-acetylcysteine on Corneal Wound Healing in a Rabbit ModelCorinna Fischak0Robert Klaus1René M. Werkmeister2Christine Hohenadl3Martin Prinz4Leopold Schmetterer5Gerhard Garhöfer6Center for Medical Physics and Biomedical Engineering, Medical University Vienna, Vienna, AustriaCenter for Medical Physics and Biomedical Engineering, Medical University Vienna, Vienna, AustriaCenter for Medical Physics and Biomedical Engineering, Medical University Vienna, Vienna, AustriaCroma Pharma, Korneuburg, AustriaCroma Pharma, Korneuburg, AustriaCenter for Medical Physics and Biomedical Engineering, Medical University Vienna, Vienna, AustriaDepartment of Clinical Pharmacology, Medical University Vienna, Vienna, AustriaPurpose. The present study was performed to investigate the effect of topically administered chitosan-N-acetylcysteine (C-NAC) on corneal wound healing in a rabbit model. Methods. A total of 20 New Zealand White rabbits were included in the randomized, masked, placebo-controlled experiment. A monocular epithelial debridement was induced by manual scraping under general anesthesia. Animals were randomized to receive either C-NAC two times daily or placebo. Monitoring of corneal wound healing was performed with ultra-high-resolution optical coherence tomography (OCT) and epithelial fluorescein staining. Measurements were done immediately after and up to 72 hours after wound induction. Results. No difference in wound size was found immediately after surgical debridement between the C-NAC group and the placebo group. Wound healing was significantly faster in the C-NAC group compared to the placebo group (p<0.01 for both methods). A good correlation was found between the OCT technique and the epithelial fluorescein staining in terms of wound size (r=0.94). Conclusions. Administration of C-NAC containing eye drops twice daily leads to a faster corneal wound healing in a rabbit model of corneal debridement as compared to placebo. Ultra-high-resolution OCT is considered a noninvasive, dye-free alternative to conventional fluorescein staining in assessing corneal wound healing also in humans.http://dx.doi.org/10.1155/2017/5192924 |
| spellingShingle | Corinna Fischak Robert Klaus René M. Werkmeister Christine Hohenadl Martin Prinz Leopold Schmetterer Gerhard Garhöfer Effect of Topically Administered Chitosan-N-acetylcysteine on Corneal Wound Healing in a Rabbit Model Journal of Ophthalmology |
| title | Effect of Topically Administered Chitosan-N-acetylcysteine on Corneal Wound Healing in a Rabbit Model |
| title_full | Effect of Topically Administered Chitosan-N-acetylcysteine on Corneal Wound Healing in a Rabbit Model |
| title_fullStr | Effect of Topically Administered Chitosan-N-acetylcysteine on Corneal Wound Healing in a Rabbit Model |
| title_full_unstemmed | Effect of Topically Administered Chitosan-N-acetylcysteine on Corneal Wound Healing in a Rabbit Model |
| title_short | Effect of Topically Administered Chitosan-N-acetylcysteine on Corneal Wound Healing in a Rabbit Model |
| title_sort | effect of topically administered chitosan n acetylcysteine on corneal wound healing in a rabbit model |
| url | http://dx.doi.org/10.1155/2017/5192924 |
| work_keys_str_mv | AT corinnafischak effectoftopicallyadministeredchitosannacetylcysteineoncornealwoundhealinginarabbitmodel AT robertklaus effectoftopicallyadministeredchitosannacetylcysteineoncornealwoundhealinginarabbitmodel AT renemwerkmeister effectoftopicallyadministeredchitosannacetylcysteineoncornealwoundhealinginarabbitmodel AT christinehohenadl effectoftopicallyadministeredchitosannacetylcysteineoncornealwoundhealinginarabbitmodel AT martinprinz effectoftopicallyadministeredchitosannacetylcysteineoncornealwoundhealinginarabbitmodel AT leopoldschmetterer effectoftopicallyadministeredchitosannacetylcysteineoncornealwoundhealinginarabbitmodel AT gerhardgarhofer effectoftopicallyadministeredchitosannacetylcysteineoncornealwoundhealinginarabbitmodel |