Nosocomial Isolates and Their Drug Resistant Pattern in ICU Patients at National Institute of Neurological and Allied Sciences, Nepal
Multidrug resistant organisms are increasing day by day and the cause is poorly known. This study was carried out from June 2011 to May 2012 at National Institute of Neurological and Allied Sciences Kathmandu, Nepal, with a view to determining drug resistant pathogens along with detection of extende...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2015/572163 |
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| author | Pashupati Bhandari Ganesh Thapa Bharat Mani Pokhrel Dwij Raj Bhatta Upendra Devkota |
| author_facet | Pashupati Bhandari Ganesh Thapa Bharat Mani Pokhrel Dwij Raj Bhatta Upendra Devkota |
| author_sort | Pashupati Bhandari |
| collection | DOAJ |
| description | Multidrug resistant organisms are increasing day by day and the cause is poorly known. This study was carried out from June 2011 to May 2012 at National Institute of Neurological and Allied Sciences Kathmandu, Nepal, with a view to determining drug resistant pathogens along with detection of extended spectrum β-lactamase (ESBL), AmpC β-lactamase (ABL), and metallo-β-lactamase (MBL) producing bacteria causing infection to ICU patients. A standard methodology was used to achieve these objectives as per recommendation of American Society for Microbiology. ESBL was detected by combined disc assay using cefotaxime and cefotaxime clavulanic acid, ABL by inhibitor based method using cefoxitin and phenylboronic acid, and MBL by imipenem-EDTA combined disk method. Two hundred and ninety-four different clinical samples such as tracheal aspirates, urine, pus, swabs, catheter tips, and blood were processed during the study. Most common bacteria were Acinetobacter spp. Of the total 58 Acinetobacter spp., 46 (79%) were MDR, and 27% were positive for ABL and 12% were for MBL. Of the 32 cases of Staphylococcus aureus, 18 (56%) were MDR. Findings of this study warrant routine β-lactamase testing in clinical isolates. |
| format | Article |
| id | doaj-art-c292e02a71d143d592025e92b1ce1610 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-c292e02a71d143d592025e92b1ce16102025-02-03T05:47:53ZengWileyInternational Journal of Microbiology1687-918X1687-91982015-01-01201510.1155/2015/572163572163Nosocomial Isolates and Their Drug Resistant Pattern in ICU Patients at National Institute of Neurological and Allied Sciences, NepalPashupati Bhandari0Ganesh Thapa1Bharat Mani Pokhrel2Dwij Raj Bhatta3Upendra Devkota4Central Department of Microbiology, Tribhuvan University, Kathmandu 44601, NepalNational Institute of Neurological and Allied Sciences (NINAS), Kathmandu 3711, NepalDepartment of Microbiology, Institute of Medicine (IOM), Tribhuvan University, Kathmandu 1524, NepalCentral Department of Microbiology, Tribhuvan University, Kathmandu 44601, NepalNational Institute of Neurological and Allied Sciences (NINAS), Kathmandu 3711, NepalMultidrug resistant organisms are increasing day by day and the cause is poorly known. This study was carried out from June 2011 to May 2012 at National Institute of Neurological and Allied Sciences Kathmandu, Nepal, with a view to determining drug resistant pathogens along with detection of extended spectrum β-lactamase (ESBL), AmpC β-lactamase (ABL), and metallo-β-lactamase (MBL) producing bacteria causing infection to ICU patients. A standard methodology was used to achieve these objectives as per recommendation of American Society for Microbiology. ESBL was detected by combined disc assay using cefotaxime and cefotaxime clavulanic acid, ABL by inhibitor based method using cefoxitin and phenylboronic acid, and MBL by imipenem-EDTA combined disk method. Two hundred and ninety-four different clinical samples such as tracheal aspirates, urine, pus, swabs, catheter tips, and blood were processed during the study. Most common bacteria were Acinetobacter spp. Of the total 58 Acinetobacter spp., 46 (79%) were MDR, and 27% were positive for ABL and 12% were for MBL. Of the 32 cases of Staphylococcus aureus, 18 (56%) were MDR. Findings of this study warrant routine β-lactamase testing in clinical isolates.http://dx.doi.org/10.1155/2015/572163 |
| spellingShingle | Pashupati Bhandari Ganesh Thapa Bharat Mani Pokhrel Dwij Raj Bhatta Upendra Devkota Nosocomial Isolates and Their Drug Resistant Pattern in ICU Patients at National Institute of Neurological and Allied Sciences, Nepal International Journal of Microbiology |
| title | Nosocomial Isolates and Their Drug Resistant Pattern in ICU Patients at National Institute of Neurological and Allied Sciences, Nepal |
| title_full | Nosocomial Isolates and Their Drug Resistant Pattern in ICU Patients at National Institute of Neurological and Allied Sciences, Nepal |
| title_fullStr | Nosocomial Isolates and Their Drug Resistant Pattern in ICU Patients at National Institute of Neurological and Allied Sciences, Nepal |
| title_full_unstemmed | Nosocomial Isolates and Their Drug Resistant Pattern in ICU Patients at National Institute of Neurological and Allied Sciences, Nepal |
| title_short | Nosocomial Isolates and Their Drug Resistant Pattern in ICU Patients at National Institute of Neurological and Allied Sciences, Nepal |
| title_sort | nosocomial isolates and their drug resistant pattern in icu patients at national institute of neurological and allied sciences nepal |
| url | http://dx.doi.org/10.1155/2015/572163 |
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