Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblasts

Plant-derived exosome-like nanovesicles (ELNs) are critical mediators of cross-kingdom communication, modulating gene expression in animal cells despite their plant origin. In this study, we investigated the effects of glucoraphanin-enriched kale (GEK)-derived ELNs (GELNs) on collagen production in...

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Main Authors: Peihan Hsu, Yuriko Kamijyo, Emiri Koike, Saki Ichikawa, Yifeng Zheng, Tomohiro Ohno, Shigeru Katayama
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-02-01
Series:Frontiers in Nutrition
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Online Access:https://www.frontiersin.org/articles/10.3389/fnut.2025.1486572/full
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author Peihan Hsu
Yuriko Kamijyo
Emiri Koike
Saki Ichikawa
Yifeng Zheng
Tomohiro Ohno
Shigeru Katayama
Shigeru Katayama
author_facet Peihan Hsu
Yuriko Kamijyo
Emiri Koike
Saki Ichikawa
Yifeng Zheng
Tomohiro Ohno
Shigeru Katayama
Shigeru Katayama
author_sort Peihan Hsu
collection DOAJ
description Plant-derived exosome-like nanovesicles (ELNs) are critical mediators of cross-kingdom communication, modulating gene expression in animal cells despite their plant origin. In this study, we investigated the effects of glucoraphanin-enriched kale (GEK)-derived ELNs (GELNs) on collagen production in normal human dermal fibroblasts NB1RGB. The ELNs isolated from GEK juice powder had particle sizes similar to those of typical exosomes. GELNs increased type I collagen expression in NB1RGB cells significantly. Microarray analysis demonstrated that GELN-derived total RNA upregulated the expression of genes related to extracellular matrix formation, including those involved in collagen synthesis. Further investigation revealed that microRNA-enriched fraction of GELNs promoted collagen production by inhibiting the expression of Smad7. These findings suggest that GELNs and their microRNA content enhance collagen production through the downregulation of Smad7.
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institution Kabale University
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publishDate 2025-02-01
publisher Frontiers Media S.A.
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series Frontiers in Nutrition
spelling doaj-art-c287d1507b18426eba821007df2070bc2025-02-10T05:16:05ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2025-02-011210.3389/fnut.2025.14865721486572Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblastsPeihan Hsu0Yuriko Kamijyo1Emiri Koike2Saki Ichikawa3Yifeng Zheng4Tomohiro Ohno5Shigeru Katayama6Shigeru Katayama7Department of Agriculture, Graduate School of Science and Technology, Shinshu University, Nagano, JapanDepartment of Agriculture, Graduate School of Science and Technology, Shinshu University, Nagano, JapanDepartment of Agriculture, Graduate School of Science and Technology, Shinshu University, Nagano, JapanDepartment of Agriculture, Graduate School of Science and Technology, Shinshu University, Nagano, JapanInstitute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Nagano, JapanYakult Health Foods Co., Ltd., Oita, JapanDepartment of Agriculture, Graduate School of Science and Technology, Shinshu University, Nagano, JapanInstitute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Nagano, JapanPlant-derived exosome-like nanovesicles (ELNs) are critical mediators of cross-kingdom communication, modulating gene expression in animal cells despite their plant origin. In this study, we investigated the effects of glucoraphanin-enriched kale (GEK)-derived ELNs (GELNs) on collagen production in normal human dermal fibroblasts NB1RGB. The ELNs isolated from GEK juice powder had particle sizes similar to those of typical exosomes. GELNs increased type I collagen expression in NB1RGB cells significantly. Microarray analysis demonstrated that GELN-derived total RNA upregulated the expression of genes related to extracellular matrix formation, including those involved in collagen synthesis. Further investigation revealed that microRNA-enriched fraction of GELNs promoted collagen production by inhibiting the expression of Smad7. These findings suggest that GELNs and their microRNA content enhance collagen production through the downregulation of Smad7.https://www.frontiersin.org/articles/10.3389/fnut.2025.1486572/fullcollagenexosomesexosome-like nanovesiclesfibroblastskalemiRNA
spellingShingle Peihan Hsu
Yuriko Kamijyo
Emiri Koike
Saki Ichikawa
Yifeng Zheng
Tomohiro Ohno
Shigeru Katayama
Shigeru Katayama
Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblasts
Frontiers in Nutrition
collagen
exosomes
exosome-like nanovesicles
fibroblasts
kale
miRNA
title Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblasts
title_full Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblasts
title_fullStr Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblasts
title_full_unstemmed Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblasts
title_short Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblasts
title_sort exosome like nanovesicles derived from kale juice enhance collagen production by downregulating smad7 in human skin fibroblasts
topic collagen
exosomes
exosome-like nanovesicles
fibroblasts
kale
miRNA
url https://www.frontiersin.org/articles/10.3389/fnut.2025.1486572/full
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