Genetic Diversity of Potential Drug Resistance Markers in <i>Plasmodium vivax</i> Isolates from Panama, Mesoamerica

This study evaluated the genetic diversity and potential drug resistance markers in <i>Plasmodium vivax</i> isolates from Panama, a country in Mesoamerica, aiming to eliminate local malaria transmission. We analyzed 70 <i>P. vivax</i> samples collected between 2004 and 2020 f...

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Main Authors: Vanessa Vásquez, Ana María Santamaría, Dianik Moreno, Fergie Ruíz, Chystrie A. Rigg, Luis F. Chaves, José E. Calzada
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Pathogens
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Online Access:https://www.mdpi.com/2076-0817/14/3/231
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author Vanessa Vásquez
Ana María Santamaría
Dianik Moreno
Fergie Ruíz
Chystrie A. Rigg
Luis F. Chaves
José E. Calzada
author_facet Vanessa Vásquez
Ana María Santamaría
Dianik Moreno
Fergie Ruíz
Chystrie A. Rigg
Luis F. Chaves
José E. Calzada
author_sort Vanessa Vásquez
collection DOAJ
description This study evaluated the genetic diversity and potential drug resistance markers in <i>Plasmodium vivax</i> isolates from Panama, a country in Mesoamerica, aiming to eliminate local malaria transmission. We analyzed 70 <i>P. vivax</i> samples collected between 2004 and 2020 from endemic regions in Eastern and Western Panama, as well as imported cases. Four drug resistance genes (<i>pvcrt-o</i>, <i>pvmdr1</i>, <i>pvdhfr</i>, and <i>pvdhps</i>) were sequenced and analyzed. Our findings reveal low genetic diversity in <i>P. vivax</i> populations from Western Panama, indicating clonal expansion, while Eastern Panama exhibits higher diversity, influenced by higher transmission rates and imported cases. No mutations were detected in <i>pvcrt-o</i>, and the prevalence of <i>pvmdr1</i> mutations (Y976F and F1076L) linked to chloroquine was observed at low frequencies, primarily in imported samples. In <i>pvdhfr</i>, antifolate-resistant mutations S117N and S58R were detected in 14.3% of samples, predominantly from Eastern Panama near the Colombian border. Phylogenetic and haplotype network analyses highlighted distinct genetic clustering, supporting the influence of imported cases on local parasite diversity. These results provide a baseline for the molecular surveillance of <i>P. vivax</i> in Panama and emphasize the need for the continued monitoring of genetic diversity and drug resistance to guide regional malaria elimination efforts, particularly in areas with high cross-border migration.
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spelling doaj-art-c284b0b94c414fdbaa1db5a042c20bc82025-08-20T01:48:57ZengMDPI AGPathogens2076-08172025-02-0114323110.3390/pathogens14030231Genetic Diversity of Potential Drug Resistance Markers in <i>Plasmodium vivax</i> Isolates from Panama, MesoamericaVanessa Vásquez0Ana María Santamaría1Dianik Moreno2Fergie Ruíz3Chystrie A. Rigg4Luis F. Chaves5José E. Calzada6Departamento de Investigación en Parasitología, Instituto Conmemorativo Gorgas de Estudios de la Salud, Panamá 0816-02593, PanamaDepartamento de Investigación en Parasitología, Instituto Conmemorativo Gorgas de Estudios de la Salud, Panamá 0816-02593, PanamaLaboratorio Central de Referencia en Salud Publica, Instituto Conmemorativo Gorgas de Estudios de la Salud, Panamá 0816-02593, PanamaLaboratorio Central de Referencia en Salud Publica, Instituto Conmemorativo Gorgas de Estudios de la Salud, Panamá 0816-02593, PanamaDepartamento de Investigación en Parasitología, Instituto Conmemorativo Gorgas de Estudios de la Salud, Panamá 0816-02593, PanamaDepartment of Environmental and Occupational Health, School of Public Health, Indiana University, Bloomington, IN 47408, USADepartamento de Investigación en Parasitología, Instituto Conmemorativo Gorgas de Estudios de la Salud, Panamá 0816-02593, PanamaThis study evaluated the genetic diversity and potential drug resistance markers in <i>Plasmodium vivax</i> isolates from Panama, a country in Mesoamerica, aiming to eliminate local malaria transmission. We analyzed 70 <i>P. vivax</i> samples collected between 2004 and 2020 from endemic regions in Eastern and Western Panama, as well as imported cases. Four drug resistance genes (<i>pvcrt-o</i>, <i>pvmdr1</i>, <i>pvdhfr</i>, and <i>pvdhps</i>) were sequenced and analyzed. Our findings reveal low genetic diversity in <i>P. vivax</i> populations from Western Panama, indicating clonal expansion, while Eastern Panama exhibits higher diversity, influenced by higher transmission rates and imported cases. No mutations were detected in <i>pvcrt-o</i>, and the prevalence of <i>pvmdr1</i> mutations (Y976F and F1076L) linked to chloroquine was observed at low frequencies, primarily in imported samples. In <i>pvdhfr</i>, antifolate-resistant mutations S117N and S58R were detected in 14.3% of samples, predominantly from Eastern Panama near the Colombian border. Phylogenetic and haplotype network analyses highlighted distinct genetic clustering, supporting the influence of imported cases on local parasite diversity. These results provide a baseline for the molecular surveillance of <i>P. vivax</i> in Panama and emphasize the need for the continued monitoring of genetic diversity and drug resistance to guide regional malaria elimination efforts, particularly in areas with high cross-border migration.https://www.mdpi.com/2076-0817/14/3/231malaria<i>Plasmodium vivax</i>drug resistance genesgenetic diversityPanamaMesoamerica
spellingShingle Vanessa Vásquez
Ana María Santamaría
Dianik Moreno
Fergie Ruíz
Chystrie A. Rigg
Luis F. Chaves
José E. Calzada
Genetic Diversity of Potential Drug Resistance Markers in <i>Plasmodium vivax</i> Isolates from Panama, Mesoamerica
Pathogens
malaria
<i>Plasmodium vivax</i>
drug resistance genes
genetic diversity
Panama
Mesoamerica
title Genetic Diversity of Potential Drug Resistance Markers in <i>Plasmodium vivax</i> Isolates from Panama, Mesoamerica
title_full Genetic Diversity of Potential Drug Resistance Markers in <i>Plasmodium vivax</i> Isolates from Panama, Mesoamerica
title_fullStr Genetic Diversity of Potential Drug Resistance Markers in <i>Plasmodium vivax</i> Isolates from Panama, Mesoamerica
title_full_unstemmed Genetic Diversity of Potential Drug Resistance Markers in <i>Plasmodium vivax</i> Isolates from Panama, Mesoamerica
title_short Genetic Diversity of Potential Drug Resistance Markers in <i>Plasmodium vivax</i> Isolates from Panama, Mesoamerica
title_sort genetic diversity of potential drug resistance markers in i plasmodium vivax i isolates from panama mesoamerica
topic malaria
<i>Plasmodium vivax</i>
drug resistance genes
genetic diversity
Panama
Mesoamerica
url https://www.mdpi.com/2076-0817/14/3/231
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