Development of a Point-of-Care Immunochromatographic Lateral Flow Strip Assay for the Detection of Nipah and Hendra Viruses
Nipah virus (NiV) and Hendra virus (HeV), which both belong to the genus henipavirus, are zoonotic pathogens that cause severe systemic, neurological, and/or respiratory disease in humans and a variety of mammals. Therefore, monitoring viral prevalence in natural reservoirs and rapidly diagnosing ca...
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2025-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/17/7/1021 |
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| author | Jianjun Jia Wenjun Zhu Guodong Liu Sandra Diederich Bradley Pickering Logan Banadyga Ming Yang |
| author_facet | Jianjun Jia Wenjun Zhu Guodong Liu Sandra Diederich Bradley Pickering Logan Banadyga Ming Yang |
| author_sort | Jianjun Jia |
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| description | Nipah virus (NiV) and Hendra virus (HeV), which both belong to the genus henipavirus, are zoonotic pathogens that cause severe systemic, neurological, and/or respiratory disease in humans and a variety of mammals. Therefore, monitoring viral prevalence in natural reservoirs and rapidly diagnosing cases of henipavirus infection are critical to limiting the spread of these viruses. Current laboratory methods for detecting NiV and HeV include virus isolation, reverse transcription quantitative real-time PCR (RT-qPCR), and antigen detection via an enzyme-linked immunosorbent assay (ELISA), all of which require highly trained personnel and specialized equipment. Here, we describe the development of a point-of-care customized immunochromatographic lateral flow (ILF) assay that uses recombinant human ephrin B2 as a capture ligand on the test line and a NiV-specific monoclonal antibody (mAb) on the conjugate pad to detect NiV and HeV. The ILF assay detects NiV and HeV with a diagnostic specificity of 94.4% and has no cross-reactivity with other viruses. This rapid test may be suitable for field testing and in countries with limited laboratory resources. |
| format | Article |
| id | doaj-art-c27bbef63f35439db2a7009f9c0ccca9 |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Viruses |
| spelling | doaj-art-c27bbef63f35439db2a7009f9c0ccca92025-08-20T03:56:46ZengMDPI AGViruses1999-49152025-07-01177102110.3390/v17071021Development of a Point-of-Care Immunochromatographic Lateral Flow Strip Assay for the Detection of Nipah and Hendra VirusesJianjun Jia0Wenjun Zhu1Guodong Liu2Sandra Diederich3Bradley Pickering4Logan Banadyga5Ming Yang6National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, CanadaNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, CanadaNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, CanadaInstitute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17493 Greifswald, GermanyNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, CanadaNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, CanadaNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, CanadaNipah virus (NiV) and Hendra virus (HeV), which both belong to the genus henipavirus, are zoonotic pathogens that cause severe systemic, neurological, and/or respiratory disease in humans and a variety of mammals. Therefore, monitoring viral prevalence in natural reservoirs and rapidly diagnosing cases of henipavirus infection are critical to limiting the spread of these viruses. Current laboratory methods for detecting NiV and HeV include virus isolation, reverse transcription quantitative real-time PCR (RT-qPCR), and antigen detection via an enzyme-linked immunosorbent assay (ELISA), all of which require highly trained personnel and specialized equipment. Here, we describe the development of a point-of-care customized immunochromatographic lateral flow (ILF) assay that uses recombinant human ephrin B2 as a capture ligand on the test line and a NiV-specific monoclonal antibody (mAb) on the conjugate pad to detect NiV and HeV. The ILF assay detects NiV and HeV with a diagnostic specificity of 94.4% and has no cross-reactivity with other viruses. This rapid test may be suitable for field testing and in countries with limited laboratory resources.https://www.mdpi.com/1999-4915/17/7/1021nipah virushendra virushenipavirusmonoclonal antibodyephrin B2virus antigen detection |
| spellingShingle | Jianjun Jia Wenjun Zhu Guodong Liu Sandra Diederich Bradley Pickering Logan Banadyga Ming Yang Development of a Point-of-Care Immunochromatographic Lateral Flow Strip Assay for the Detection of Nipah and Hendra Viruses Viruses nipah virus hendra virus henipavirus monoclonal antibody ephrin B2 virus antigen detection |
| title | Development of a Point-of-Care Immunochromatographic Lateral Flow Strip Assay for the Detection of Nipah and Hendra Viruses |
| title_full | Development of a Point-of-Care Immunochromatographic Lateral Flow Strip Assay for the Detection of Nipah and Hendra Viruses |
| title_fullStr | Development of a Point-of-Care Immunochromatographic Lateral Flow Strip Assay for the Detection of Nipah and Hendra Viruses |
| title_full_unstemmed | Development of a Point-of-Care Immunochromatographic Lateral Flow Strip Assay for the Detection of Nipah and Hendra Viruses |
| title_short | Development of a Point-of-Care Immunochromatographic Lateral Flow Strip Assay for the Detection of Nipah and Hendra Viruses |
| title_sort | development of a point of care immunochromatographic lateral flow strip assay for the detection of nipah and hendra viruses |
| topic | nipah virus hendra virus henipavirus monoclonal antibody ephrin B2 virus antigen detection |
| url | https://www.mdpi.com/1999-4915/17/7/1021 |
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