MINIMUM INHIBITORY CONCENTRATION OF PURPLE LEAF EXTRACT (GRAPTOPHYLLUM PICTUM L. GRIFF) AGAINST LACTOBACILLUS ACIDOPHILUS ATCC 4356
Background: Dental caries is an oral cavity disease that affects most Indonesians. Lactobacillus acidophilus (L. acidophilus) is one of the bacteria that causes dental caries. Control of bacteria in the form of antibacterial agents is needed to suppress the growth of L. acidophilus. Purple leaves (G...
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Universitas Airlangga
2023-11-01
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| Series: | Journal of Vocational Health Studies |
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| Online Access: | https://e-journal.unair.ac.id/JVHS/article/view/43037 |
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| author | Shafira Indriyani Chusnadia Atik Kurniawati Sari Setyaningsih Erawati Wulandari Pudji Astuti |
| author_facet | Shafira Indriyani Chusnadia Atik Kurniawati Sari Setyaningsih Erawati Wulandari Pudji Astuti |
| author_sort | Shafira Indriyani Chusnadia |
| collection | DOAJ |
| description | Background: Dental caries is an oral cavity disease that affects most Indonesians. Lactobacillus acidophilus (L. acidophilus) is one of the bacteria that causes dental caries. Control of bacteria in the form of antibacterial agents is needed to suppress the growth of L. acidophilus. Purple leaves (Graptophyllum pictum L. Griff) are a medicinal plant with antibacterial compounds, namely flavonoids, alkaloids, saponins, and tannins. Purpose: Determine the Minimum Inhibitory Concentration (MIC) of purple leaves extract on the growth of L. acidophilus. Method: The sample consisted of seven groups, including positive control (chlorhexidine 0.2%), negative control (BHI-B), and purple leaves extract with concentrations of 25%, 12.5%, 6.25%, 3.12%, and 1.56%. The antibacterial activity of purple leaves extract was carried out quantitatively using a spectrophotometer with a wavelength of 600 nm. After that, it was incubated at 37°C for 48 hours, followed by absorbance measurement. The absorbance results were then analyzed using the Paired T-Test (before and after incubation). Result: Purple leaves extract concentrations of 6.25%, 12.5%, and 25% had an inhibitory effect on L. acidophillus. Conclusion: Minimum Inhibitory Concentration (MIC) of purple leaves extract on the growth of L. acidophilus was 6.25% |
| format | Article |
| id | doaj-art-c276f7fe2f3b4ccfb6f1c20f33b6ee3b |
| institution | Kabale University |
| issn | 2580-7161 2580-717X |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal of Vocational Health Studies |
| spelling | doaj-art-c276f7fe2f3b4ccfb6f1c20f33b6ee3b2025-02-03T05:48:14ZengUniversitas AirlanggaJournal of Vocational Health Studies2580-71612580-717X2023-11-017211512010.20473/jvhs.V7.I2.2023.115-12041119MINIMUM INHIBITORY CONCENTRATION OF PURPLE LEAF EXTRACT (GRAPTOPHYLLUM PICTUM L. GRIFF) AGAINST LACTOBACILLUS ACIDOPHILUS ATCC 4356Shafira Indriyani Chusnadia0https://orcid.org/0009-0009-3821-7348Atik Kurniawati1https://orcid.org/0000-0003-4731-6320Sari Setyaningsih2https://orcid.org/0000-0002-7300-3734Erawati Wulandari3https://orcid.org/0000-0001-9268-5200Pudji Astuti4https://orcid.org/0009-0000-7526-1983Oral Biology Department, Faculty of Dentistry, Jember UniversityOral Biology Department, Faculty of Dentistry, Jember UniversityBiomedic Department, Faculty of Dentistry, Jember UniversityConservative Dentistry Department, Faculty of Dentistry, Jember UniversityBiomedic Department, Faculty of Dentistry, Jember UniversityBackground: Dental caries is an oral cavity disease that affects most Indonesians. Lactobacillus acidophilus (L. acidophilus) is one of the bacteria that causes dental caries. Control of bacteria in the form of antibacterial agents is needed to suppress the growth of L. acidophilus. Purple leaves (Graptophyllum pictum L. Griff) are a medicinal plant with antibacterial compounds, namely flavonoids, alkaloids, saponins, and tannins. Purpose: Determine the Minimum Inhibitory Concentration (MIC) of purple leaves extract on the growth of L. acidophilus. Method: The sample consisted of seven groups, including positive control (chlorhexidine 0.2%), negative control (BHI-B), and purple leaves extract with concentrations of 25%, 12.5%, 6.25%, 3.12%, and 1.56%. The antibacterial activity of purple leaves extract was carried out quantitatively using a spectrophotometer with a wavelength of 600 nm. After that, it was incubated at 37°C for 48 hours, followed by absorbance measurement. The absorbance results were then analyzed using the Paired T-Test (before and after incubation). Result: Purple leaves extract concentrations of 6.25%, 12.5%, and 25% had an inhibitory effect on L. acidophillus. Conclusion: Minimum Inhibitory Concentration (MIC) of purple leaves extract on the growth of L. acidophilus was 6.25%https://e-journal.unair.ac.id/JVHS/article/view/43037lactobacillus acidophiluspurple leaves extractmicdental caries |
| spellingShingle | Shafira Indriyani Chusnadia Atik Kurniawati Sari Setyaningsih Erawati Wulandari Pudji Astuti MINIMUM INHIBITORY CONCENTRATION OF PURPLE LEAF EXTRACT (GRAPTOPHYLLUM PICTUM L. GRIFF) AGAINST LACTOBACILLUS ACIDOPHILUS ATCC 4356 Journal of Vocational Health Studies lactobacillus acidophilus purple leaves extract mic dental caries |
| title | MINIMUM INHIBITORY CONCENTRATION OF PURPLE LEAF EXTRACT (GRAPTOPHYLLUM PICTUM L. GRIFF) AGAINST LACTOBACILLUS ACIDOPHILUS ATCC 4356 |
| title_full | MINIMUM INHIBITORY CONCENTRATION OF PURPLE LEAF EXTRACT (GRAPTOPHYLLUM PICTUM L. GRIFF) AGAINST LACTOBACILLUS ACIDOPHILUS ATCC 4356 |
| title_fullStr | MINIMUM INHIBITORY CONCENTRATION OF PURPLE LEAF EXTRACT (GRAPTOPHYLLUM PICTUM L. GRIFF) AGAINST LACTOBACILLUS ACIDOPHILUS ATCC 4356 |
| title_full_unstemmed | MINIMUM INHIBITORY CONCENTRATION OF PURPLE LEAF EXTRACT (GRAPTOPHYLLUM PICTUM L. GRIFF) AGAINST LACTOBACILLUS ACIDOPHILUS ATCC 4356 |
| title_short | MINIMUM INHIBITORY CONCENTRATION OF PURPLE LEAF EXTRACT (GRAPTOPHYLLUM PICTUM L. GRIFF) AGAINST LACTOBACILLUS ACIDOPHILUS ATCC 4356 |
| title_sort | minimum inhibitory concentration of purple leaf extract graptophyllum pictum l griff against lactobacillus acidophilus atcc 4356 |
| topic | lactobacillus acidophilus purple leaves extract mic dental caries |
| url | https://e-journal.unair.ac.id/JVHS/article/view/43037 |
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