Determination of verapamil through LC-ESI-MS/MS in a case of fatal intoxication
Verapamil is a synthetic derivative of papaverine, which is used therapeutically as a hypertensive, antiarrhythmic and antianginal. This study describes an analytical method for the determination of verapamil in biological matrices of blood and urine, which consists of a liquid-liquid extraction of...
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| Language: | English |
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Universidad Nacional de Colombia
2015-07-01
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| Series: | Case Reports |
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| Online Access: | https://revistas.unal.edu.co/index.php/care/article/view/49461 |
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| author | Sindy Varón Diana Mariño |
| author_facet | Sindy Varón Diana Mariño |
| author_sort | Sindy Varón |
| collection | DOAJ |
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Verapamil is a synthetic derivative of papaverine, which is used therapeutically as a hypertensive, antiarrhythmic and antianginal. This study describes an analytical method for the determination of verapamil in biological matrices of blood and urine, which consists of a liquid-liquid extraction of samples for analysis using liquid chromatography-mass spectrometry (LC-ESI-MS/ MS), with flurazepam as an internal standard. The method was applied to the acute fatal intoxication of a 17-year-old young woman who consumed 170 tablets of verapamil; the concentration of this medication found in the blood was 18.261mg/L and 0.369mg/L in the urine. This study also puts forth the use of LC-ESI-MS/MS in the analysis of verapamil in biological samples for applications in forensic toxicology.
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| format | Article |
| id | doaj-art-c276d3035c994f3c9e545f4c8ae6e277 |
| institution | DOAJ |
| issn | 2462-8522 |
| language | English |
| publishDate | 2015-07-01 |
| publisher | Universidad Nacional de Colombia |
| record_format | Article |
| series | Case Reports |
| spelling | doaj-art-c276d3035c994f3c9e545f4c8ae6e2772025-08-20T02:48:10ZengUniversidad Nacional de ColombiaCase Reports2462-85222015-07-011241902Determination of verapamil through LC-ESI-MS/MS in a case of fatal intoxicationSindy Varón0Diana Mariño1National Institute of Legal Medicine and Forensic Science, Bogotá Office,National Institute of Legal Medicine and Forensic Science, Bogotá Office, Verapamil is a synthetic derivative of papaverine, which is used therapeutically as a hypertensive, antiarrhythmic and antianginal. This study describes an analytical method for the determination of verapamil in biological matrices of blood and urine, which consists of a liquid-liquid extraction of samples for analysis using liquid chromatography-mass spectrometry (LC-ESI-MS/ MS), with flurazepam as an internal standard. The method was applied to the acute fatal intoxication of a 17-year-old young woman who consumed 170 tablets of verapamil; the concentration of this medication found in the blood was 18.261mg/L and 0.369mg/L in the urine. This study also puts forth the use of LC-ESI-MS/MS in the analysis of verapamil in biological samples for applications in forensic toxicology. https://revistas.unal.edu.co/index.php/care/article/view/49461VerapamilLiquid chromatography with electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS)Forensic toxicology |
| spellingShingle | Sindy Varón Diana Mariño Determination of verapamil through LC-ESI-MS/MS in a case of fatal intoxication Case Reports Verapamil Liquid chromatography with electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS) Forensic toxicology |
| title | Determination of verapamil through LC-ESI-MS/MS in a case of fatal intoxication |
| title_full | Determination of verapamil through LC-ESI-MS/MS in a case of fatal intoxication |
| title_fullStr | Determination of verapamil through LC-ESI-MS/MS in a case of fatal intoxication |
| title_full_unstemmed | Determination of verapamil through LC-ESI-MS/MS in a case of fatal intoxication |
| title_short | Determination of verapamil through LC-ESI-MS/MS in a case of fatal intoxication |
| title_sort | determination of verapamil through lc esi ms ms in a case of fatal intoxication |
| topic | Verapamil Liquid chromatography with electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS) Forensic toxicology |
| url | https://revistas.unal.edu.co/index.php/care/article/view/49461 |
| work_keys_str_mv | AT sindyvaron determinationofverapamilthroughlcesimsmsinacaseoffatalintoxication AT dianamarino determinationofverapamilthroughlcesimsmsinacaseoffatalintoxication |