Induced pluripotent stem cell–related approaches to generate dopaminergic neurons for Parkinson’s disease
The progressive loss of dopaminergic neurons in affected patient brains is one of the pathological features of Parkinson’s disease, the second most common human neurodegenerative disease. Although the detailed pathogenesis accounting for dopaminergic neuron degeneration in Parkinson’s disease is sti...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-11-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/NRR.NRR-D-24-00771 |
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| _version_ | 1841556306961367040 |
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| author | Ling-Xiao Yi Hui Ren Woon Genevieve Saw Li Zeng Eng King Tan Zhi Dong Zhou |
| author_facet | Ling-Xiao Yi Hui Ren Woon Genevieve Saw Li Zeng Eng King Tan Zhi Dong Zhou |
| author_sort | Ling-Xiao Yi |
| collection | DOAJ |
| description | The progressive loss of dopaminergic neurons in affected patient brains is one of the pathological features of Parkinson’s disease, the second most common human neurodegenerative disease. Although the detailed pathogenesis accounting for dopaminergic neuron degeneration in Parkinson’s disease is still unclear, the advancement of stem cell approaches has shown promise for Parkinson’s disease research and therapy. The induced pluripotent stem cells have been commonly used to generate dopaminergic neurons, which has provided valuable insights to improve our understanding of Parkinson’s disease pathogenesis and contributed to anti-Parkinson’s disease therapies. The current review discusses the practical approaches and potential applications of induced pluripotent stem cell techniques for generating and differentiating dopaminergic neurons from induced pluripotent stem cells. The benefits of induced pluripotent stem cell-based research are highlighted. Various dopaminergic neuron differentiation protocols from induced pluripotent stem cells are compared. The emerging three-dimension-based brain organoid models compared with conventional two-dimensional cell culture are evaluated. Finally, limitations, challenges, and future directions of induced pluripotent stem cell–based approaches are analyzed and proposed, which will be significant to the future application of induced pluripotent stem cell–related techniques for Parkinson’s disease. |
| format | Article |
| id | doaj-art-c2733d2eeeef48f587c055ddfe93c695 |
| institution | Kabale University |
| issn | 1673-5374 1876-7958 |
| language | English |
| publishDate | 2025-11-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj-art-c2733d2eeeef48f587c055ddfe93c6952025-01-07T09:49:28ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53741876-79582025-11-0120113193320610.4103/NRR.NRR-D-24-00771Induced pluripotent stem cell–related approaches to generate dopaminergic neurons for Parkinson’s diseaseLing-Xiao YiHui Ren WoonGenevieve SawLi ZengEng King TanZhi Dong ZhouThe progressive loss of dopaminergic neurons in affected patient brains is one of the pathological features of Parkinson’s disease, the second most common human neurodegenerative disease. Although the detailed pathogenesis accounting for dopaminergic neuron degeneration in Parkinson’s disease is still unclear, the advancement of stem cell approaches has shown promise for Parkinson’s disease research and therapy. The induced pluripotent stem cells have been commonly used to generate dopaminergic neurons, which has provided valuable insights to improve our understanding of Parkinson’s disease pathogenesis and contributed to anti-Parkinson’s disease therapies. The current review discusses the practical approaches and potential applications of induced pluripotent stem cell techniques for generating and differentiating dopaminergic neurons from induced pluripotent stem cells. The benefits of induced pluripotent stem cell-based research are highlighted. Various dopaminergic neuron differentiation protocols from induced pluripotent stem cells are compared. The emerging three-dimension-based brain organoid models compared with conventional two-dimensional cell culture are evaluated. Finally, limitations, challenges, and future directions of induced pluripotent stem cell–based approaches are analyzed and proposed, which will be significant to the future application of induced pluripotent stem cell–related techniques for Parkinson’s disease.https://journals.lww.com/10.4103/NRR.NRR-D-24-00771dopaminergic neuronsinduced pluripotent stem cellsparkinson’s diseasestem cell approaches |
| spellingShingle | Ling-Xiao Yi Hui Ren Woon Genevieve Saw Li Zeng Eng King Tan Zhi Dong Zhou Induced pluripotent stem cell–related approaches to generate dopaminergic neurons for Parkinson’s disease Neural Regeneration Research dopaminergic neurons induced pluripotent stem cells parkinson’s disease stem cell approaches |
| title | Induced pluripotent stem cell–related approaches to generate dopaminergic neurons for Parkinson’s disease |
| title_full | Induced pluripotent stem cell–related approaches to generate dopaminergic neurons for Parkinson’s disease |
| title_fullStr | Induced pluripotent stem cell–related approaches to generate dopaminergic neurons for Parkinson’s disease |
| title_full_unstemmed | Induced pluripotent stem cell–related approaches to generate dopaminergic neurons for Parkinson’s disease |
| title_short | Induced pluripotent stem cell–related approaches to generate dopaminergic neurons for Parkinson’s disease |
| title_sort | induced pluripotent stem cell related approaches to generate dopaminergic neurons for parkinson s disease |
| topic | dopaminergic neurons induced pluripotent stem cells parkinson’s disease stem cell approaches |
| url | https://journals.lww.com/10.4103/NRR.NRR-D-24-00771 |
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