Supercharged nanoadhesive through co-assembly of recombinant protein and tetrahedral DNA for corneal transplantation
A traumatic tissue adhesive technology is highly sought after in ophthalmic surgery; however, many polymeric adhesives face significant limitations in clinical ophthalmology, particularly in corneal transplantation. A major challenge is achieving rapid adhesion without introducing polymer barriers o...
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| Format: | Article |
| Language: | English |
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IOP Publishing
2025-01-01
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| Series: | Materials Futures |
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| Online Access: | https://doi.org/10.1088/2752-5724/adac80 |
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| author | Jing Zhao Feng Zhang Zhe Zhang Zhenhua Li Yanze Yu Bingqing Sun Binghong Yu Yong Ma Xuan Ding Liyin Wang Lifei Zheng Wenguo Cui Xingtao Zhou |
| author_facet | Jing Zhao Feng Zhang Zhe Zhang Zhenhua Li Yanze Yu Bingqing Sun Binghong Yu Yong Ma Xuan Ding Liyin Wang Lifei Zheng Wenguo Cui Xingtao Zhou |
| author_sort | Jing Zhao |
| collection | DOAJ |
| description | A traumatic tissue adhesive technology is highly sought after in ophthalmic surgery; however, many polymeric adhesives face significant limitations in clinical ophthalmology, particularly in corneal transplantation. A major challenge is achieving rapid adhesion without introducing polymer barriers or chemical toxicity from cross-linking. To address this, we developed a novel cornea-specific nanoadhesive constructed through protein-DNA co-assembly and applied it to corneal transplantation. In this system, a rigid tetrahedral DNA framework was employed to guide the spatial distribution of polycationic recombinant proteins (K72) and serve as the core of the nanoadhesive, facilitating energy conversion during tissue connection. The adhesive demonstrated a strength of 2.3 kPa between corneal lenticules. After modification with RGD peptides, the adhesive system significantly enhanced corneal epithelialization, reduced inflammation and neovascularization, and ultimately promoted corneal repair. This study represents the first application of a nanoadhesive in ophthalmic surgery, providing a novel solution for developing ophthalmic-specific adhesives for clinical use. |
| format | Article |
| id | doaj-art-c26eb70192d64aa989ca87fa11d29222 |
| institution | Kabale University |
| issn | 2752-5724 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | Materials Futures |
| spelling | doaj-art-c26eb70192d64aa989ca87fa11d292222025-02-07T13:24:22ZengIOP PublishingMaterials Futures2752-57242025-01-014101500210.1088/2752-5724/adac80Supercharged nanoadhesive through co-assembly of recombinant protein and tetrahedral DNA for corneal transplantationJing Zhao0Feng Zhang1Zhe Zhang2Zhenhua Li3Yanze Yu4Bingqing Sun5Binghong Yu6Yong Ma7Xuan Ding8Liyin Wang9Lifei Zheng10Wenguo Cui11https://orcid.org/0000-0002-6938-9582Xingtao Zhou12Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University , Shanghai 200031, People’s Republic of China; Key Laboratory of Myopia and Related Eye Diseases, NHC; Key laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences , Shanghai 200031, People’s Republic of China; Shanghai Research Center of Ophthalmology and Optometry , Shanghai 200031, People’s Republic of China; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care , Shanghai 200031, People’s Republic of ChinaEye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University , Shanghai 200031, People’s Republic of China; Key Laboratory of Myopia and Related Eye Diseases, NHC; Key laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences , Shanghai 200031, People’s Republic of China; Shanghai Research Center of Ophthalmology and Optometry , Shanghai 200031, People’s Republic of China; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care , Shanghai 200031, People’s Republic of ChinaEye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University , Shanghai 200031, People’s Republic of China; Key Laboratory of Myopia and Related Eye Diseases, NHC; Key laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences , Shanghai 200031, People’s Republic of China; Shanghai Research Center of Ophthalmology and Optometry , Shanghai 200031, People’s Republic of China; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care , Shanghai 200031, People’s Republic of ChinaWenzhou Institute, University of Chinese Academy of Sciences , Wenzhou, Zhejiang, People’s Republic of ChinaEye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University , Shanghai 200031, People’s Republic of China; Key Laboratory of Myopia and Related Eye Diseases, NHC; Key laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences , Shanghai 200031, People’s Republic of China; Shanghai Research Center of Ophthalmology and Optometry , Shanghai 200031, People’s Republic of China; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care , Shanghai 200031, People’s Republic of ChinaEye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University , Shanghai 200031, People’s Republic of China; Key Laboratory of Myopia and Related Eye Diseases, NHC; Key laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences , Shanghai 200031, People’s Republic of China; Shanghai Research Center of Ophthalmology and Optometry , Shanghai 200031, People’s Republic of China; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care , Shanghai 200031, People’s Republic of ChinaWenzhou Institute, University of Chinese Academy of Sciences , Wenzhou, Zhejiang, People’s Republic of ChinaEye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University , Shanghai 200031, People’s Republic of China; Key Laboratory of Myopia and Related Eye Diseases, NHC; Key laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences , Shanghai 200031, People’s Republic of China; Shanghai Research Center of Ophthalmology and Optometry , Shanghai 200031, People’s Republic of China; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care , Shanghai 200031, People’s Republic of ChinaEye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University , Shanghai 200031, People’s Republic of China; Key Laboratory of Myopia and Related Eye Diseases, NHC; Key laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences , Shanghai 200031, People’s Republic of China; Shanghai Research Center of Ophthalmology and Optometry , Shanghai 200031, People’s Republic of China; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care , Shanghai 200031, People’s Republic of ChinaEye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University , Shanghai 200031, People’s Republic of China; Key Laboratory of Myopia and Related Eye Diseases, NHC; Key laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences , Shanghai 200031, People’s Republic of China; Shanghai Research Center of Ophthalmology and Optometry , Shanghai 200031, People’s Republic of China; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care , Shanghai 200031, People’s Republic of ChinaWenzhou Institute, University of Chinese Academy of Sciences , Wenzhou, Zhejiang, People’s Republic of ChinaDepartment of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine , 197 Ruijin 2nd Road, Shanghai 200025, People’s Republic of ChinaEye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University , Shanghai 200031, People’s Republic of China; Key Laboratory of Myopia and Related Eye Diseases, NHC; Key laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences , Shanghai 200031, People’s Republic of China; Shanghai Research Center of Ophthalmology and Optometry , Shanghai 200031, People’s Republic of China; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care , Shanghai 200031, People’s Republic of ChinaA traumatic tissue adhesive technology is highly sought after in ophthalmic surgery; however, many polymeric adhesives face significant limitations in clinical ophthalmology, particularly in corneal transplantation. A major challenge is achieving rapid adhesion without introducing polymer barriers or chemical toxicity from cross-linking. To address this, we developed a novel cornea-specific nanoadhesive constructed through protein-DNA co-assembly and applied it to corneal transplantation. In this system, a rigid tetrahedral DNA framework was employed to guide the spatial distribution of polycationic recombinant proteins (K72) and serve as the core of the nanoadhesive, facilitating energy conversion during tissue connection. The adhesive demonstrated a strength of 2.3 kPa between corneal lenticules. After modification with RGD peptides, the adhesive system significantly enhanced corneal epithelialization, reduced inflammation and neovascularization, and ultimately promoted corneal repair. This study represents the first application of a nanoadhesive in ophthalmic surgery, providing a novel solution for developing ophthalmic-specific adhesives for clinical use.https://doi.org/10.1088/2752-5724/adac80biomacromolecule-based nanoadhesivecorneal transplantationsupercharged proteintetrahedral DNAbiosafety |
| spellingShingle | Jing Zhao Feng Zhang Zhe Zhang Zhenhua Li Yanze Yu Bingqing Sun Binghong Yu Yong Ma Xuan Ding Liyin Wang Lifei Zheng Wenguo Cui Xingtao Zhou Supercharged nanoadhesive through co-assembly of recombinant protein and tetrahedral DNA for corneal transplantation Materials Futures biomacromolecule-based nanoadhesive corneal transplantation supercharged protein tetrahedral DNA biosafety |
| title | Supercharged nanoadhesive through co-assembly of recombinant protein and tetrahedral DNA for corneal transplantation |
| title_full | Supercharged nanoadhesive through co-assembly of recombinant protein and tetrahedral DNA for corneal transplantation |
| title_fullStr | Supercharged nanoadhesive through co-assembly of recombinant protein and tetrahedral DNA for corneal transplantation |
| title_full_unstemmed | Supercharged nanoadhesive through co-assembly of recombinant protein and tetrahedral DNA for corneal transplantation |
| title_short | Supercharged nanoadhesive through co-assembly of recombinant protein and tetrahedral DNA for corneal transplantation |
| title_sort | supercharged nanoadhesive through co assembly of recombinant protein and tetrahedral dna for corneal transplantation |
| topic | biomacromolecule-based nanoadhesive corneal transplantation supercharged protein tetrahedral DNA biosafety |
| url | https://doi.org/10.1088/2752-5724/adac80 |
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