Detection of Salmonella and several common Salmonella serotypes in food by loop-mediated isothermal amplification method
Salmonella Choleraesuis, S. Enteritidis and S. Typhimurium are the main pathogens that contaminate animal products and cause human Salmonella food poisoning. To establish the loop-mediated isothermal amplification (LAMP) method for the rapid detection of Salmonella and 3 common Salmonella serotypes,...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2015-06-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453015000270 |
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| author | Zhongqiang Chen Ke Zhang Huan Yin Qi Li Lan Wang Zhiguo Liu |
| author_facet | Zhongqiang Chen Ke Zhang Huan Yin Qi Li Lan Wang Zhiguo Liu |
| author_sort | Zhongqiang Chen |
| collection | DOAJ |
| description | Salmonella Choleraesuis, S. Enteritidis and S. Typhimurium are the main pathogens that contaminate animal products and cause human Salmonella food poisoning. To establish the loop-mediated isothermal amplification (LAMP) method for the rapid detection of Salmonella and 3 common Salmonella serotypes, inner and outer primer sets targeting Salmonella invE gene and 3 serotype-specific genes fliC, lygD and STM4495 were designed. The LAMP reaction conditions were optimized. The specificity of LAMP primers was identified by testing 10 different bacterial strains including S. Choleraesuis, S. Enteritidis and S. Typhimurium. Take S. Choleraesuis as example, the detection limit of LAMP assay was 1.33 × 101 CFU/mL for bacteria culture and 2.0 × 101 CFU/mL for simulated pork sample. The results show that LAMP is a rapid, sensitive and specific method for Salmonella detection and can be used for the rapid detection of Salmonella in food. |
| format | Article |
| id | doaj-art-c26e94305e1a46a0b22996f65f786f41 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2015-06-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-c26e94305e1a46a0b22996f65f786f412025-02-03T10:36:30ZengTsinghua University PressFood Science and Human Wellness2213-45302015-06-0142757910.1016/j.fshw.2015.05.001Detection of Salmonella and several common Salmonella serotypes in food by loop-mediated isothermal amplification methodZhongqiang ChenKe ZhangHuan YinQi LiLan WangZhiguo LiuSalmonella Choleraesuis, S. Enteritidis and S. Typhimurium are the main pathogens that contaminate animal products and cause human Salmonella food poisoning. To establish the loop-mediated isothermal amplification (LAMP) method for the rapid detection of Salmonella and 3 common Salmonella serotypes, inner and outer primer sets targeting Salmonella invE gene and 3 serotype-specific genes fliC, lygD and STM4495 were designed. The LAMP reaction conditions were optimized. The specificity of LAMP primers was identified by testing 10 different bacterial strains including S. Choleraesuis, S. Enteritidis and S. Typhimurium. Take S. Choleraesuis as example, the detection limit of LAMP assay was 1.33 × 101 CFU/mL for bacteria culture and 2.0 × 101 CFU/mL for simulated pork sample. The results show that LAMP is a rapid, sensitive and specific method for Salmonella detection and can be used for the rapid detection of Salmonella in food.http://www.sciencedirect.com/science/article/pii/S2213453015000270Loop-mediated isothermal amplificationDetectionSalmonellaSalmonella CholeraesuisSalmonella EnteritidisSalmonella Typhimurium |
| spellingShingle | Zhongqiang Chen Ke Zhang Huan Yin Qi Li Lan Wang Zhiguo Liu Detection of Salmonella and several common Salmonella serotypes in food by loop-mediated isothermal amplification method Food Science and Human Wellness Loop-mediated isothermal amplification Detection Salmonella Salmonella Choleraesuis Salmonella Enteritidis Salmonella Typhimurium |
| title | Detection of Salmonella and several common Salmonella serotypes in food by loop-mediated isothermal amplification method |
| title_full | Detection of Salmonella and several common Salmonella serotypes in food by loop-mediated isothermal amplification method |
| title_fullStr | Detection of Salmonella and several common Salmonella serotypes in food by loop-mediated isothermal amplification method |
| title_full_unstemmed | Detection of Salmonella and several common Salmonella serotypes in food by loop-mediated isothermal amplification method |
| title_short | Detection of Salmonella and several common Salmonella serotypes in food by loop-mediated isothermal amplification method |
| title_sort | detection of salmonella and several common salmonella serotypes in food by loop mediated isothermal amplification method |
| topic | Loop-mediated isothermal amplification Detection Salmonella Salmonella Choleraesuis Salmonella Enteritidis Salmonella Typhimurium |
| url | http://www.sciencedirect.com/science/article/pii/S2213453015000270 |
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